Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype
The complexity of the sugarcane genome, the narrow genetic base of subtropical germplasm, and the extended breeding and selection cycle pose significant challenges to conventional breeding methods. In vitro mutagenesis offers an alternative approach to generating genetic variability by harnessing bo...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer Nature
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/23188 https://link.springer.com/article/10.1007/s44372-025-00298-x https://doi.org/10.1007/s44372-025-00298-x |
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| author | Di Pauli, Valentina Fontana, Paola Daniela Lewi, Dalia Marcela Felipe, Arturo Erazzu, Luis Ernesto |
| author_browse | Di Pauli, Valentina Erazzu, Luis Ernesto Felipe, Arturo Fontana, Paola Daniela Lewi, Dalia Marcela |
| author_facet | Di Pauli, Valentina Fontana, Paola Daniela Lewi, Dalia Marcela Felipe, Arturo Erazzu, Luis Ernesto |
| author_sort | Di Pauli, Valentina |
| collection | INTA Digital |
| description | The complexity of the sugarcane genome, the narrow genetic base of subtropical germplasm, and the extended breeding and selection cycle pose significant challenges to conventional breeding methods. In vitro mutagenesis offers an alternative approach to generating genetic variability by harnessing both somaclonal variation and induced mutations. An in vitro mutagenesis protocol, using the chemical mutagen ethyl methanesulfonate (EMS) in an elite Argentine sugarcane cultivar, was developed. The resulting genetic variability was assessed in the field in plant and first ratoon crops. Six-week-old embryogenic calli were treated with 8, 16, 32, and 48 mM EMS. Plants were regenerated from treated calli and acclimatized in a greenhouse. The lethal dose LD50 for regeneration capacity was 31.68 mM EMS, whereas LD25 was 17.71 mM EMS. Based on these results, the plants from the 16 and 32 mM EMS treatments were phenotypically assessed in the field. These assessments showed that EMS treatments exhibited phenotypic variability due to genetic changes. Traits such as the number of stalks, stalk length, stalk weight, internode length, and Brix showed an increase in the population mean in the EMS treatments compared with the wild type. Some traits maintained changes from one year to the next, whereas others reverted to the wild-type genotype values in the second year. Mutagenic treatments generated greater and more stable genetic variation than somaclonal variation, supporting their use as effective tools for sugarcane improvement. Based on agronomic traits, 23 mutant plants were selected and advanced to subsequent generations. |
| format | info:ar-repo/semantics/artículo |
| id | INTA23188 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INTA231882025-07-28T16:12:09Z Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype Di Pauli, Valentina Fontana, Paola Daniela Lewi, Dalia Marcela Felipe, Arturo Erazzu, Luis Ernesto Caña de Azúcar Genotipos Variación Genética Ems Argentina Sugar Cane Genotypes Genetic Variation Mutagenesis EMS Etilmetanosulfonato Ethylmethanesulphonate The complexity of the sugarcane genome, the narrow genetic base of subtropical germplasm, and the extended breeding and selection cycle pose significant challenges to conventional breeding methods. In vitro mutagenesis offers an alternative approach to generating genetic variability by harnessing both somaclonal variation and induced mutations. An in vitro mutagenesis protocol, using the chemical mutagen ethyl methanesulfonate (EMS) in an elite Argentine sugarcane cultivar, was developed. The resulting genetic variability was assessed in the field in plant and first ratoon crops. Six-week-old embryogenic calli were treated with 8, 16, 32, and 48 mM EMS. Plants were regenerated from treated calli and acclimatized in a greenhouse. The lethal dose LD50 for regeneration capacity was 31.68 mM EMS, whereas LD25 was 17.71 mM EMS. Based on these results, the plants from the 16 and 32 mM EMS treatments were phenotypically assessed in the field. These assessments showed that EMS treatments exhibited phenotypic variability due to genetic changes. Traits such as the number of stalks, stalk length, stalk weight, internode length, and Brix showed an increase in the population mean in the EMS treatments compared with the wild type. Some traits maintained changes from one year to the next, whereas others reverted to the wild-type genotype values in the second year. Mutagenic treatments generated greater and more stable genetic variation than somaclonal variation, supporting their use as effective tools for sugarcane improvement. Based on agronomic traits, 23 mutant plants were selected and advanced to subsequent generations. EEA Famaillá Fil: Di Pauli, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Fontana, Paola Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Felipe, Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Erazzú, Luis E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. 2025-07-28T16:09:29Z 2025-07-28T16:09:29Z 2025-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23188 https://link.springer.com/article/10.1007/s44372-025-00298-x 3005-1207 https://doi.org/10.1007/s44372-025-00298-x eng info:eu-repograntAgreement/INTA/2023-PD-L01-I071, Mejoramiento genético de los Cultivos Industriales y estrategias alternativas para la obtención de biotipos adaptados a los nuevos escenarios y al cambio climático info:eu-repograntAgreement/INTA/2023-PD-L01-I086, Edición génica, transgénesis y mutaciones inducidas como generadores de nueva variabilidad en especies de interés agropecuario info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Nature Discover Plants 2 : article number 230. (July 2025) |
| spellingShingle | Caña de Azúcar Genotipos Variación Genética Ems Argentina Sugar Cane Genotypes Genetic Variation Mutagenesis EMS Etilmetanosulfonato Ethylmethanesulphonate Di Pauli, Valentina Fontana, Paola Daniela Lewi, Dalia Marcela Felipe, Arturo Erazzu, Luis Ernesto Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype |
| title | Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype |
| title_full | Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype |
| title_fullStr | Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype |
| title_full_unstemmed | Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype |
| title_short | Ethyl methanesulfonate-induced mutagenesis generates genetic and morphological variations in an elite Argentine sugarcane genotype |
| title_sort | ethyl methanesulfonate induced mutagenesis generates genetic and morphological variations in an elite argentine sugarcane genotype |
| topic | Caña de Azúcar Genotipos Variación Genética Ems Argentina Sugar Cane Genotypes Genetic Variation Mutagenesis EMS Etilmetanosulfonato Ethylmethanesulphonate |
| url | http://hdl.handle.net/20.500.12123/23188 https://link.springer.com/article/10.1007/s44372-025-00298-x https://doi.org/10.1007/s44372-025-00298-x |
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