Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows
Bovine leukemia virus (BLV) is a retrovirus that infects cattle, causing bovine enzootic leukosis, a chronic disease characterized by the proliferation of infected B cells. BLV proviral load (PVL) is a key determinant of disease progression and transmission risk. Cattle can exhibit distinct phenotyp...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Frontiers Media
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/23157 https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1550646/full https://doi.org/10.3389/fvets.2025.1550646 |
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| author | Petersen, Marcos Iván Suarez Archilla, Guillermo Miretti, Marcos Mateo Trono, Karina Gabriela Carignano, Hugo Adrian |
| author_browse | Carignano, Hugo Adrian Miretti, Marcos Mateo Petersen, Marcos Iván Suarez Archilla, Guillermo Trono, Karina Gabriela |
| author_facet | Petersen, Marcos Iván Suarez Archilla, Guillermo Miretti, Marcos Mateo Trono, Karina Gabriela Carignano, Hugo Adrian |
| author_sort | Petersen, Marcos Iván |
| collection | INTA Digital |
| description | Bovine leukemia virus (BLV) is a retrovirus that infects cattle, causing bovine enzootic leukosis, a chronic disease characterized by the proliferation of infected B cells. BLV proviral load (PVL) is a key determinant of disease progression and transmission risk. Cattle can exhibit distinct phenotypes of low PVL (LPVL) or high PVL (HPVL), which remain stable throughout their lifetime. Differential expression analysis revealed 1,908 differentially expressed genes (DEGs) between HPVL and LPVL animals, including 774 downregulated (DReg) and 1,134 upregulated (UReg) genes. Functional enrichment analysis revealed that DReg genes were associated primarily with immune response pathways. Conversely, the UReg genes were enriched in processes related to cell cycle regulation, mitotic division, and DNA biosynthesis. Protein–protein interaction analysis revealed six highly interconnected clusters. Interestingly, a cluster was enriched for sphingolipid metabolism, a process critical to enveloped virus infection and immune receptor signaling. These findings provide valuable insights into the molecular mechanisms of BLV infection, suggesting potential markers for disease monitoring and targets for therapeutic intervention. |
| format | info:ar-repo/semantics/artículo |
| id | INTA23157 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA231572025-07-24T13:46:39Z Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows Petersen, Marcos Iván Suarez Archilla, Guillermo Miretti, Marcos Mateo Trono, Karina Gabriela Carignano, Hugo Adrian Bovine Leukemia Virus Gene Expression RNA Sequence Transcriptome Cows Immune Response Proviruses Virus Leucemia Bovina Expresión Génica Secuencia de ARN Transcriptoma Vaca Respuesta Inmunológica Provirus Bovine leukemia virus (BLV) is a retrovirus that infects cattle, causing bovine enzootic leukosis, a chronic disease characterized by the proliferation of infected B cells. BLV proviral load (PVL) is a key determinant of disease progression and transmission risk. Cattle can exhibit distinct phenotypes of low PVL (LPVL) or high PVL (HPVL), which remain stable throughout their lifetime. Differential expression analysis revealed 1,908 differentially expressed genes (DEGs) between HPVL and LPVL animals, including 774 downregulated (DReg) and 1,134 upregulated (UReg) genes. Functional enrichment analysis revealed that DReg genes were associated primarily with immune response pathways. Conversely, the UReg genes were enriched in processes related to cell cycle regulation, mitotic division, and DNA biosynthesis. Protein–protein interaction analysis revealed six highly interconnected clusters. Interestingly, a cluster was enriched for sphingolipid metabolism, a process critical to enveloped virus infection and immune receptor signaling. These findings provide valuable insights into the molecular mechanisms of BLV infection, suggesting potential markers for disease monitoring and targets for therapeutic intervention. Instituto de Virología Fil: Petersen, Marcos Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Petersen, Marcos Iván. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Miretti, Marcos Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Miretti, Marcos Mateo. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical. Grupo de Investigación en Genética Aplicada; Argentina Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Trono, Karina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Carignano, Hugo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-07-24T13:40:03Z 2025-07-24T13:40:03Z 2025-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23157 https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1550646/full 2297-1769 https://doi.org/10.3389/fvets.2025.1550646 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Veterinary Science 12 : 1550646 (April 2025) |
| spellingShingle | Bovine Leukemia Virus Gene Expression RNA Sequence Transcriptome Cows Immune Response Proviruses Virus Leucemia Bovina Expresión Génica Secuencia de ARN Transcriptoma Vaca Respuesta Inmunológica Provirus Petersen, Marcos Iván Suarez Archilla, Guillermo Miretti, Marcos Mateo Trono, Karina Gabriela Carignano, Hugo Adrian Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows |
| title | Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows |
| title_full | Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows |
| title_fullStr | Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows |
| title_full_unstemmed | Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows |
| title_short | Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows |
| title_sort | whole transcriptome analysis of blv infected cows reveals downregulation of immune response genes in high proviral loads cows |
| topic | Bovine Leukemia Virus Gene Expression RNA Sequence Transcriptome Cows Immune Response Proviruses Virus Leucemia Bovina Expresión Génica Secuencia de ARN Transcriptoma Vaca Respuesta Inmunológica Provirus |
| url | http://hdl.handle.net/20.500.12123/23157 https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1550646/full https://doi.org/10.3389/fvets.2025.1550646 |
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