Surveillance of leptospiral reservoirs in synanthropic rodents using loop-mediated isothermal amplification
Leptospirosis, a zoonotic disease caused by pathogenic Leptospira spp., represents a major public health concern due to its impact on both rural and urban populations. Rodents, particularly Rattus norvegicus, Rattus rattus, and Mus musculus, are key reservoirs, excreting leptospires in their urine a...
| Main Authors: | , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/23134 https://www.sciencedirect.com/science/article/abs/pii/S1090023325000449 https://doi.org/10.1016/j.tvjl.2025.106340 |
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| author | Hamer, Micaela Saraullo, Vanina Rosa Muschetto, Emiliano Esteban, Micaela Tripodi, Mariel Alejandra Sanchez, Maria Cristina Hancke, Diego Suárez, Olga Virginia Brihuega, Bibiana Felicitas Martinez, Mara Leila |
| author_browse | Brihuega, Bibiana Felicitas Esteban, Micaela Hamer, Micaela Hancke, Diego Martinez, Mara Leila Muschetto, Emiliano Sanchez, Maria Cristina Saraullo, Vanina Rosa Suárez, Olga Virginia Tripodi, Mariel Alejandra |
| author_facet | Hamer, Micaela Saraullo, Vanina Rosa Muschetto, Emiliano Esteban, Micaela Tripodi, Mariel Alejandra Sanchez, Maria Cristina Hancke, Diego Suárez, Olga Virginia Brihuega, Bibiana Felicitas Martinez, Mara Leila |
| author_sort | Hamer, Micaela |
| collection | INTA Digital |
| description | Leptospirosis, a zoonotic disease caused by pathogenic Leptospira spp., represents a major public health concern due to its impact on both rural and urban populations. Rodents, particularly Rattus norvegicus, Rattus rattus, and Mus musculus, are key reservoirs, excreting leptospires in their urine and contributing to environmental contamination. In this study, we evaluated the efficacy of loop-mediated isothermal amplification (LAMP), a molecular diagnostic tool, for detecting leptospiral DNA in kidney samples from captured rodents. LAMP results were compared with the standard lipL32 PCR assay. Leptospiral DNA was detected in 9.0 % (14/156) of samples, with 5.8 % positive by both LAMP and lipL32 PCR and 3.2 % positive by LAMP alone. No samples were positive by PCR and negative by LAMP. Cohen's Kappa index (0.77) indicated substantial agreement between the two methods. The higher sensitivity of LAMP, its ability to detect both pathogenic and intermediate leptospiral strains, and its cost-effectiveness make it a valuable tool for low-resource settings. However, the technique’s inability to differentiate between Leptospira species highlights the need for complementary methods for epidemiological studies. These findings contribute to the understanding of rodent leptospirosis reservoirs and offer practical diagnostic solutions for veterinary and public health surveillance. |
| format | Artículo |
| id | INTA23134 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA231342025-07-23T14:30:01Z Surveillance of leptospiral reservoirs in synanthropic rodents using loop-mediated isothermal amplification Hamer, Micaela Saraullo, Vanina Rosa Muschetto, Emiliano Esteban, Micaela Tripodi, Mariel Alejandra Sanchez, Maria Cristina Hancke, Diego Suárez, Olga Virginia Brihuega, Bibiana Felicitas Martinez, Mara Leila Diagnostic Techniques Public Health Zoonoses Leptospirosis Leptospira Rodents Técnica de Diagnóstico Salud Pública Zoonosis Isothermal Processes Proceso Isotérmico Roedor Leptospirosis, a zoonotic disease caused by pathogenic Leptospira spp., represents a major public health concern due to its impact on both rural and urban populations. Rodents, particularly Rattus norvegicus, Rattus rattus, and Mus musculus, are key reservoirs, excreting leptospires in their urine and contributing to environmental contamination. In this study, we evaluated the efficacy of loop-mediated isothermal amplification (LAMP), a molecular diagnostic tool, for detecting leptospiral DNA in kidney samples from captured rodents. LAMP results were compared with the standard lipL32 PCR assay. Leptospiral DNA was detected in 9.0 % (14/156) of samples, with 5.8 % positive by both LAMP and lipL32 PCR and 3.2 % positive by LAMP alone. No samples were positive by PCR and negative by LAMP. Cohen's Kappa index (0.77) indicated substantial agreement between the two methods. The higher sensitivity of LAMP, its ability to detect both pathogenic and intermediate leptospiral strains, and its cost-effectiveness make it a valuable tool for low-resource settings. However, the technique’s inability to differentiate between Leptospira species highlights the need for complementary methods for epidemiological studies. These findings contribute to the understanding of rodent leptospirosis reservoirs and offer practical diagnostic solutions for veterinary and public health surveillance. Instituto de Patobiología Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Muschetto, Emiliano. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Ecología de Roedores Urbanos; Argentina Fil: Muschetto, Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tripodi, Mariel Alejandra. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Ecología de Roedores Urbanos; Argentina Fil: Tripodi, Mariel Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hancke, Diego. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Ecología de Roedores Urbanos; Argentina Fil: Hancke, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Suárez, Olga Virginia. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Ecología de Roedores Urbanos; Argentina Fil: Suárez, Olga Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Escuela de Veterinaria; Argentina Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinez, Mara Leila. Universidad del Salvador. Escuela de Veterinaria; Argentina 2025-07-23T14:22:00Z 2025-07-23T14:22:00Z 2025-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23134 https://www.sciencedirect.com/science/article/abs/pii/S1090023325000449 1090-0233 https://doi.org/10.1016/j.tvjl.2025.106340 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier The Veterinary Journal 311 : 106340 (June 2025) |
| spellingShingle | Diagnostic Techniques Public Health Zoonoses Leptospirosis Leptospira Rodents Técnica de Diagnóstico Salud Pública Zoonosis Isothermal Processes Proceso Isotérmico Roedor Hamer, Micaela Saraullo, Vanina Rosa Muschetto, Emiliano Esteban, Micaela Tripodi, Mariel Alejandra Sanchez, Maria Cristina Hancke, Diego Suárez, Olga Virginia Brihuega, Bibiana Felicitas Martinez, Mara Leila Surveillance of leptospiral reservoirs in synanthropic rodents using loop-mediated isothermal amplification |
| title | Surveillance of leptospiral reservoirs in synanthropic rodents using loop-mediated isothermal amplification |
| title_full | Surveillance of leptospiral reservoirs in synanthropic rodents using loop-mediated isothermal amplification |
| title_fullStr | Surveillance of leptospiral reservoirs in synanthropic rodents using loop-mediated isothermal amplification |
| title_full_unstemmed | Surveillance of leptospiral reservoirs in synanthropic rodents using loop-mediated isothermal amplification |
| title_short | Surveillance of leptospiral reservoirs in synanthropic rodents using loop-mediated isothermal amplification |
| title_sort | surveillance of leptospiral reservoirs in synanthropic rodents using loop mediated isothermal amplification |
| topic | Diagnostic Techniques Public Health Zoonoses Leptospirosis Leptospira Rodents Técnica de Diagnóstico Salud Pública Zoonosis Isothermal Processes Proceso Isotérmico Roedor |
| url | http://hdl.handle.net/20.500.12123/23134 https://www.sciencedirect.com/science/article/abs/pii/S1090023325000449 https://doi.org/10.1016/j.tvjl.2025.106340 |
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