Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs
Background: Antimicrobial resistance has become a significant global issue impacting humans, animals, and the environment. Currently, the focus of concern has shifted to the environment, which can act as a reservoir and significantly contribute to the spread of resistance genes. This study aimed to...
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| Format: | Artículo |
| Language: | Inglés |
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BioMed Central
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/23111 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-025-04665-4 https://doi.org/10.1186/s12917-025-04665-4 |
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| author | Dominguez, Johanna Elizabeth Martino, Florencia Lovera, Rosario Casanova, Natalia Andrea Seah, Christine Cavia, Regino Corso, Alejandra Melano, Roberto G. Faccone, Diego Fernandez Miyakawa, Mariano Enrique |
| author_browse | Casanova, Natalia Andrea Cavia, Regino Corso, Alejandra Dominguez, Johanna Elizabeth Faccone, Diego Fernandez Miyakawa, Mariano Enrique Lovera, Rosario Martino, Florencia Melano, Roberto G. Seah, Christine |
| author_facet | Dominguez, Johanna Elizabeth Martino, Florencia Lovera, Rosario Casanova, Natalia Andrea Seah, Christine Cavia, Regino Corso, Alejandra Melano, Roberto G. Faccone, Diego Fernandez Miyakawa, Mariano Enrique |
| author_sort | Dominguez, Johanna Elizabeth |
| collection | INTA Digital |
| description | Background: Antimicrobial resistance has become a significant global issue impacting humans, animals, and the environment. Currently, the focus of concern has shifted to the environment, which can act as a reservoir and significantly contribute to the spread of resistance genes. This study aimed to elucidate the potential transmission of mcr-1, which confers colistin resistance, among Escherichia coli isolates from pigs, dairy cattle, and co-habiting rodents. In March 2018, 30 fecal samples were collected from three pig farms and one mixed cattle farm, and 31 cecal contents from rats (Rattus norvegicus) captured from the same four animal farms were analyzed.
Results: Out of 26 mcr-1 positive E. coli isolates, 16 came from six rats, 10 from four pigs, and none from dairy cattle. The mcr-1-positive isolates from cohabiting rats and pigs were genetically unrelated, based on different XbaI-PFGE profiles. The plasmid profiles of one isolate per animal from each farm were analyzed by S1-PFGE. E. coli isolates from cohabiting rats and pigs showed plasmid bands of similar sizes (33 or 65 kb). To investigate the horizontal transfer of these plasmids between the animals, two pairs of E. coli isolates from pig farms 1 and 3 were selected for WGS analysis. Three of the isolates (EcoP3-1, EcoC2-1 from pigs, and Eco1266-6 from a rat) belonged to clonal complex 10 (CC10), while the other rat isolate (Eco1284-6) belonged to CC398 (ST398). Eco1266-6 (rat) and EcoC2-1 (pig) from cohabiting animals in pig farm 1 carried IncX4 plasmids with the mcr-1.1 variant. The plasmid sequences were almost identical (99.98% identity), both carrying the mcr-1.1/pap2 segment. pEcoC2-1 had a complete ISVsa5 insertion sequence upstream of the mcr-1 gene. Eco1284-6 (rat) and EcoP3-1 (pig) from pig farm 3 carried IncI2 plasmids with different allelic variants of mcr-1 (mcr-1.5 and mcr-1.1).
Conclusions: E. coli isolates from cohabiting rats and pigs were genetically distinct, but one pair of isolates had very similar IncX4 plasmids, suggesting the potential for horizontal spread of plasmids carrying mcr genes. These findings suggest a threat of resistant E. coli spreading between cohabiting animals and into the environment. This underscores the importance of conducting integrated One-Health studies. |
| format | Artículo |
| id | INTA23111 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | BioMed Central |
| publisherStr | BioMed Central |
| record_format | dspace |
| spelling | INTA231112025-07-23T10:16:16Z Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs Dominguez, Johanna Elizabeth Martino, Florencia Lovera, Rosario Casanova, Natalia Andrea Seah, Christine Cavia, Regino Corso, Alejandra Melano, Roberto G. Faccone, Diego Fernandez Miyakawa, Mariano Enrique Antimicrobial Resistance Resistance Genes Colistin Plasmids Rats Dairy Cattle Swine One Health Approach Resistencia a los Antimicrobianos Escherichia coli Gen de Resistencia Colistina Plasmidios Rata Ganado de Leche Cerdo Rattus norvegicus Enfoque Una Salud Background: Antimicrobial resistance has become a significant global issue impacting humans, animals, and the environment. Currently, the focus of concern has shifted to the environment, which can act as a reservoir and significantly contribute to the spread of resistance genes. This study aimed to elucidate the potential transmission of mcr-1, which confers colistin resistance, among Escherichia coli isolates from pigs, dairy cattle, and co-habiting rodents. In March 2018, 30 fecal samples were collected from three pig farms and one mixed cattle farm, and 31 cecal contents from rats (Rattus norvegicus) captured from the same four animal farms were analyzed. Results: Out of 26 mcr-1 positive E. coli isolates, 16 came from six rats, 10 from four pigs, and none from dairy cattle. The mcr-1-positive isolates from cohabiting rats and pigs were genetically unrelated, based on different XbaI-PFGE profiles. The plasmid profiles of one isolate per animal from each farm were analyzed by S1-PFGE. E. coli isolates from cohabiting rats and pigs showed plasmid bands of similar sizes (33 or 65 kb). To investigate the horizontal transfer of these plasmids between the animals, two pairs of E. coli isolates from pig farms 1 and 3 were selected for WGS analysis. Three of the isolates (EcoP3-1, EcoC2-1 from pigs, and Eco1266-6 from a rat) belonged to clonal complex 10 (CC10), while the other rat isolate (Eco1284-6) belonged to CC398 (ST398). Eco1266-6 (rat) and EcoC2-1 (pig) from cohabiting animals in pig farm 1 carried IncX4 plasmids with the mcr-1.1 variant. The plasmid sequences were almost identical (99.98% identity), both carrying the mcr-1.1/pap2 segment. pEcoC2-1 had a complete ISVsa5 insertion sequence upstream of the mcr-1 gene. Eco1284-6 (rat) and EcoP3-1 (pig) from pig farm 3 carried IncI2 plasmids with different allelic variants of mcr-1 (mcr-1.5 and mcr-1.1). Conclusions: E. coli isolates from cohabiting rats and pigs were genetically distinct, but one pair of isolates had very similar IncX4 plasmids, suggesting the potential for horizontal spread of plasmids carrying mcr genes. These findings suggest a threat of resistant E. coli spreading between cohabiting animals and into the environment. This underscores the importance of conducting integrated One-Health studies. Instituto de Patobiología Fil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Bacteriología General; Argentina Fil: Dominguez, Johana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martino, Florencia. Instituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr. Carlos G. Malbran". Servicio de Antimicrobianos; Argentina Fil: Martino, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lovera, Rosario. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Lovera, Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Casanova, Natalia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Bacteriología General; Argentina Fil: Casanova, Natalia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Seah, Christine. Public Health Ontario Laboratory; Canadá Fil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución; Argentina Fil: Cavia, Regino. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Corso, Alejandra. Instituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr. Carlos G. Malbran". Servicio Antimicrobianos; Argentina Fil: Melano, Roberto G. Public Health Ontario Laboratory; Canadá Fil: Faccone, Diego. Instituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr. Carlos G. Malbran". Servicio de Antimicrobianos; Argentina Fil: Faccone, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Bacteriología General; Argentina Fil: Fernandez Miyakawa, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-07-23T10:09:42Z 2025-07-23T10:09:42Z 2025-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23111 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-025-04665-4 1746-6148 https://doi.org/10.1186/s12917-025-04665-4 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BioMed Central BMC Veterinary Research 21 : 271 (April 2025) |
| spellingShingle | Antimicrobial Resistance Resistance Genes Colistin Plasmids Rats Dairy Cattle Swine One Health Approach Resistencia a los Antimicrobianos Escherichia coli Gen de Resistencia Colistina Plasmidios Rata Ganado de Leche Cerdo Rattus norvegicus Enfoque Una Salud Dominguez, Johanna Elizabeth Martino, Florencia Lovera, Rosario Casanova, Natalia Andrea Seah, Christine Cavia, Regino Corso, Alejandra Melano, Roberto G. Faccone, Diego Fernandez Miyakawa, Mariano Enrique Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs |
| title | Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs |
| title_full | Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs |
| title_fullStr | Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs |
| title_full_unstemmed | Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs |
| title_short | Genomic characterization of plasmids of mcr-1-positive Escherichia coli isolated from cohabiting rats, dairy cattle and pigs |
| title_sort | genomic characterization of plasmids of mcr 1 positive escherichia coli isolated from cohabiting rats dairy cattle and pigs |
| topic | Antimicrobial Resistance Resistance Genes Colistin Plasmids Rats Dairy Cattle Swine One Health Approach Resistencia a los Antimicrobianos Escherichia coli Gen de Resistencia Colistina Plasmidios Rata Ganado de Leche Cerdo Rattus norvegicus Enfoque Una Salud |
| url | http://hdl.handle.net/20.500.12123/23111 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-025-04665-4 https://doi.org/10.1186/s12917-025-04665-4 |
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