Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice
Background: Bovine tuberculosis (bTB) is a disease primarily caused by Mycobacterium bovis. Currently, no commercial vaccines exist for controlling bTB, making the development of effective vaccine candidates and testing models a high priority. Mouse models are widely used in preclinical trials of an...
| Main Authors: | , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Wolters Kluwer Medknow
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/23108 https://journals.lww.com/ijmy/fulltext/2025/04000/dynamics_of_the_in_vitro_growing_of_mycobacterium.8.aspx https://doi.org/10.4103/ijmy.ijmy_32_25 |
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| author | Blanco, Federico Carlos Klepp, Laura Ines Vazquez, Cristina Lourdes Bigi, Fabiana |
| author_browse | Bigi, Fabiana Blanco, Federico Carlos Klepp, Laura Ines Vazquez, Cristina Lourdes |
| author_facet | Blanco, Federico Carlos Klepp, Laura Ines Vazquez, Cristina Lourdes Bigi, Fabiana |
| author_sort | Blanco, Federico Carlos |
| collection | INTA Digital |
| description | Background: Bovine tuberculosis (bTB) is a disease primarily caused by Mycobacterium bovis. Currently, no commercial vaccines exist for controlling bTB, making the development of effective vaccine candidates and testing models a high priority. Mouse models are widely used in preclinical trials of anti-TB vaccines. Determining the appropriate cultivation time to assess the mycobacterial load in animal organs or biological samples is crucial to establishing a reliable model that can accurately evaluate the effectiveness of a vaccine candidate. The aim of this study was to assess the growth dynamics and the appearance of colony-forming units (CFUs) in lung homogenates from mice infected with M. bovis. We compared the CFU counts from vaccinated and challenged mice with M. bovis using data from a previous experiment.
Methods: CFUs obtained from the lungs of vaccinated and M. bovis-challenged mice of a previous experiment were registered at 3 and 4 weeks of culturing in solid media. The statistical analysis was performed with Kruskal–Wallis, followed by a Dunn’s multiple comparison test.
Results: On analyzing the CFU dynamics from lung homogenates, we found that mice vaccinated with Bacillus Calmette–Guérin preserved stable CFU counts after 3 weeks of cultivation on a solid medium. In contrast, both the unvaccinated group and the group vaccinated with an attenuated M. bovis triple mutant strain reached their final CFU counts only after 4 weeks of culturing.
Conclusion: These findings underscore the importance of prolonged follow-up to accurately assess CFU counts, which are crucial for determining vaccine efficacy in trials. |
| format | Artículo |
| id | INTA23108 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wolters Kluwer Medknow |
| publisherStr | Wolters Kluwer Medknow |
| record_format | dspace |
| spelling | INTA231082025-07-21T15:05:16Z Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice Blanco, Federico Carlos Klepp, Laura Ines Vazquez, Cristina Lourdes Bigi, Fabiana Mycobacterium bovis Vaccines In vitro Lungs Mice Vacuna Pulmones Ratón Background: Bovine tuberculosis (bTB) is a disease primarily caused by Mycobacterium bovis. Currently, no commercial vaccines exist for controlling bTB, making the development of effective vaccine candidates and testing models a high priority. Mouse models are widely used in preclinical trials of anti-TB vaccines. Determining the appropriate cultivation time to assess the mycobacterial load in animal organs or biological samples is crucial to establishing a reliable model that can accurately evaluate the effectiveness of a vaccine candidate. The aim of this study was to assess the growth dynamics and the appearance of colony-forming units (CFUs) in lung homogenates from mice infected with M. bovis. We compared the CFU counts from vaccinated and challenged mice with M. bovis using data from a previous experiment. Methods: CFUs obtained from the lungs of vaccinated and M. bovis-challenged mice of a previous experiment were registered at 3 and 4 weeks of culturing in solid media. The statistical analysis was performed with Kruskal–Wallis, followed by a Dunn’s multiple comparison test. Results: On analyzing the CFU dynamics from lung homogenates, we found that mice vaccinated with Bacillus Calmette–Guérin preserved stable CFU counts after 3 weeks of cultivation on a solid medium. In contrast, both the unvaccinated group and the group vaccinated with an attenuated M. bovis triple mutant strain reached their final CFU counts only after 4 weeks of culturing. Conclusion: These findings underscore the importance of prolonged follow-up to accurately assess CFU counts, which are crucial for determining vaccine efficacy in trials. Instituto de Biotecnología Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Vazquez, Cristina Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-07-21T14:57:55Z 2025-07-21T14:57:55Z 2025-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23108 https://journals.lww.com/ijmy/fulltext/2025/04000/dynamics_of_the_in_vitro_growing_of_mycobacterium.8.aspx 2212-5531 https://doi.org/10.4103/ijmy.ijmy_32_25 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wolters Kluwer Medknow International Journal of Mycobacteriology 14 (2) : 140-144 (Apr-Jun 2025) |
| spellingShingle | Mycobacterium bovis Vaccines In vitro Lungs Mice Vacuna Pulmones Ratón Blanco, Federico Carlos Klepp, Laura Ines Vazquez, Cristina Lourdes Bigi, Fabiana Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice |
| title | Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice |
| title_full | Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice |
| title_fullStr | Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice |
| title_full_unstemmed | Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice |
| title_short | Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice |
| title_sort | dynamics of the in vitro growing of mycobacterium bovis from the lungs of vaccinated and infected mice |
| topic | Mycobacterium bovis Vaccines In vitro Lungs Mice Vacuna Pulmones Ratón |
| url | http://hdl.handle.net/20.500.12123/23108 https://journals.lww.com/ijmy/fulltext/2025/04000/dynamics_of_the_in_vitro_growing_of_mycobacterium.8.aspx https://doi.org/10.4103/ijmy.ijmy_32_25 |
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