Neutrophils from tuberculosis patients are polarized toward pro-inflammatory and anti-inflammatory phenotypes according to the disease severity
Neutrophils are the first line of defense against pathogens, combating them by using several antimicrobial mechanisms. These cells display a remarkable plasticity that can be molded by the different environments that neutrophils confront to protect the host, therefore presenting diverse phenotypes....
| Autores principales: | , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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American Association of Immunologists
2025
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/23105 https://academic.oup.com/jimmunol/article/214/6/1173/8106431 https://doi.org/10.1093/jimmun/vkaf010 |
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| author | Morelli, María Paula Martin, Candela Pellegrini, Joaquín Miguel Blanco, Federico Carlos Bigi, Fabiana Ciallella, Lorena Musella, Rosa María Rodriguez Mieres, Adriana de Casado, Graciela C. Palmero, Domingo García, Verónica Edith |
| author_browse | Bigi, Fabiana Blanco, Federico Carlos Ciallella, Lorena García, Verónica Edith Martin, Candela Morelli, María Paula Musella, Rosa María Palmero, Domingo Pellegrini, Joaquín Miguel Rodriguez Mieres, Adriana de Casado, Graciela C. |
| author_facet | Morelli, María Paula Martin, Candela Pellegrini, Joaquín Miguel Blanco, Federico Carlos Bigi, Fabiana Ciallella, Lorena Musella, Rosa María Rodriguez Mieres, Adriana de Casado, Graciela C. Palmero, Domingo García, Verónica Edith |
| author_sort | Morelli, María Paula |
| collection | INTA Digital |
| description | Neutrophils are the first line of defense against pathogens, combating them by using several antimicrobial mechanisms. These cells display a remarkable plasticity that can be molded by the different environments that neutrophils confront to protect the host, therefore presenting diverse phenotypes. Actually, pro- and anti-inflammatory neutrophils populations (N1- and N2-like phenotypes) have been described in cancer and inflammatory disorders. However, the identification of N1/N2 neutrophil subtypes in human intracellular bacterial diseases remains unexplored. Here, we characterized neutrophils from tuberculosis (TB) patients presenting distinct immunological status according to their disease severity. TB patients were classified as high or low responders (HR or LR) in accordance with their immunity against Mycobacterium tuberculosis (Mtb). Interestingly, by analyzing the phenotypic and functional characteristics of neutrophils from the two groups of TB patients we demonstrated that HR patient’s neutrophils display a pro-inflammatory N1-like phenotype, whereas LR patient’s neutrophils show an anti-inflammatory N2-like phenotype. Remarkably, whereas neutrophils from both groups of patients phagocytized MtbH37Rv strain equally, HR TB’s neutrophils displayed a significantly increased ability to kill pathogenic Mtb as compared to neutrophils from LR TB patients that presented a diminished capacity of bacterial elimination. Together, our findings suggest the existence of different subtypes of neutrophils in TB patients according to their immune response to Mtb and disease severity, indicating that neutrophils might be promising targets for TB host-directed therapy. |
| format | info:ar-repo/semantics/artículo |
| id | INTA23105 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | American Association of Immunologists |
| publisherStr | American Association of Immunologists |
| record_format | dspace |
| spelling | INTA231052025-07-21T14:34:34Z Neutrophils from tuberculosis patients are polarized toward pro-inflammatory and anti-inflammatory phenotypes according to the disease severity Morelli, María Paula Martin, Candela Pellegrini, Joaquín Miguel Blanco, Federico Carlos Bigi, Fabiana Ciallella, Lorena Musella, Rosa María Rodriguez Mieres, Adriana de Casado, Graciela C. Palmero, Domingo García, Verónica Edith Cytokines Neutrophils Antiinflammatory Agents Inflammation Phenotypes Disease Severity Pathogenicity Immune Response Citoquinas Neutrófilos Tuberculosis Antinflamatorios Inflamación Fenotipos Gravedad de una Enfermedad Mycobacterium tuberculosis Patogenicidad Respuesta Inmunológica Neutrophils are the first line of defense against pathogens, combating them by using several antimicrobial mechanisms. These cells display a remarkable plasticity that can be molded by the different environments that neutrophils confront to protect the host, therefore presenting diverse phenotypes. Actually, pro- and anti-inflammatory neutrophils populations (N1- and N2-like phenotypes) have been described in cancer and inflammatory disorders. However, the identification of N1/N2 neutrophil subtypes in human intracellular bacterial diseases remains unexplored. Here, we characterized neutrophils from tuberculosis (TB) patients presenting distinct immunological status according to their disease severity. TB patients were classified as high or low responders (HR or LR) in accordance with their immunity against Mycobacterium tuberculosis (Mtb). Interestingly, by analyzing the phenotypic and functional characteristics of neutrophils from the two groups of TB patients we demonstrated that HR patient’s neutrophils display a pro-inflammatory N1-like phenotype, whereas LR patient’s neutrophils show an anti-inflammatory N2-like phenotype. Remarkably, whereas neutrophils from both groups of patients phagocytized MtbH37Rv strain equally, HR TB’s neutrophils displayed a significantly increased ability to kill pathogenic Mtb as compared to neutrophils from LR TB patients that presented a diminished capacity of bacterial elimination. Together, our findings suggest the existence of different subtypes of neutrophils in TB patients according to their immune response to Mtb and disease severity, indicating that neutrophils might be promising targets for TB host-directed therapy. Instituto de Biotecnología Fil: Morelli, María Paula. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina Fil: Martin, Candela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina Fil: Martin, Candela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pellegrini, Joaquín Miguel. Parc Scientifique et Technologique de Luminy. Aix-Marseille Université. Centre National de la Recherche Scientifique (CNRS). Centre d'Immunologie de Marseille Luminy; Francia Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Bigi, Fabiana. Consejo Nacional de investigaciones Científicas y Técnicas; Argentina Fil: Ciallella, Lorena. Hospital F. J. Muñiz. División Tisioneumonología; Argentina Fil: Musella, Rosa María. Hospital F. J. Muñiz. División Tisioneumonología; Argentina Fil: Rodriguez Mieres, Adriana. Hospital F. J. Muñiz. División Tisioneumonología; Argentina Fil: de Casado, Graciela C. Hospital F. J. Muñiz. División Tisioneumonología; Argentina Fil: Palmero, Domingo. Hospital F. J. Muñiz. División Tisioneumonología; Argentina Fil: García, Verónica Edith. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina Fil: García, Verónica Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-07-21T14:27:30Z 2025-07-21T14:27:30Z 2025-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23105 https://academic.oup.com/jimmunol/article/214/6/1173/8106431 1550-6606 https://doi.org/10.1093/jimmun/vkaf010 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Association of Immunologists The Journal of Immunology 214 (6) : 1173-1186 (June 2025) |
| spellingShingle | Cytokines Neutrophils Antiinflammatory Agents Inflammation Phenotypes Disease Severity Pathogenicity Immune Response Citoquinas Neutrófilos Tuberculosis Antinflamatorios Inflamación Fenotipos Gravedad de una Enfermedad Mycobacterium tuberculosis Patogenicidad Respuesta Inmunológica Morelli, María Paula Martin, Candela Pellegrini, Joaquín Miguel Blanco, Federico Carlos Bigi, Fabiana Ciallella, Lorena Musella, Rosa María Rodriguez Mieres, Adriana de Casado, Graciela C. Palmero, Domingo García, Verónica Edith Neutrophils from tuberculosis patients are polarized toward pro-inflammatory and anti-inflammatory phenotypes according to the disease severity |
| title | Neutrophils from tuberculosis patients are polarized toward pro-inflammatory and anti-inflammatory phenotypes according to the disease severity |
| title_full | Neutrophils from tuberculosis patients are polarized toward pro-inflammatory and anti-inflammatory phenotypes according to the disease severity |
| title_fullStr | Neutrophils from tuberculosis patients are polarized toward pro-inflammatory and anti-inflammatory phenotypes according to the disease severity |
| title_full_unstemmed | Neutrophils from tuberculosis patients are polarized toward pro-inflammatory and anti-inflammatory phenotypes according to the disease severity |
| title_short | Neutrophils from tuberculosis patients are polarized toward pro-inflammatory and anti-inflammatory phenotypes according to the disease severity |
| title_sort | neutrophils from tuberculosis patients are polarized toward pro inflammatory and anti inflammatory phenotypes according to the disease severity |
| topic | Cytokines Neutrophils Antiinflammatory Agents Inflammation Phenotypes Disease Severity Pathogenicity Immune Response Citoquinas Neutrófilos Tuberculosis Antinflamatorios Inflamación Fenotipos Gravedad de una Enfermedad Mycobacterium tuberculosis Patogenicidad Respuesta Inmunológica |
| url | http://hdl.handle.net/20.500.12123/23105 https://academic.oup.com/jimmunol/article/214/6/1173/8106431 https://doi.org/10.1093/jimmun/vkaf010 |
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