Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil
We identified a novel plant rhabdovirus infecting native joá (Solanum aculeatissimum) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The vir...
| Main Authors: | , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2025
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/23082 https://link.springer.com/article/10.1007/s00705-021-05040-y https://doi.org/10.1007/s00705-021-05040-y |
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| author | Dietzgen, Ralf Georg Bejerman, Nicolas Esteban Yongyu, Mei Jee, Charmaine Lim Jing Chabi-Jesus, Camila Freitas-Astúa, Juliana Veras, Solange M. Kitajima, Elliot W. |
| author_browse | Bejerman, Nicolas Esteban Chabi-Jesus, Camila Dietzgen, Ralf Georg Freitas-Astúa, Juliana Jee, Charmaine Lim Jing Kitajima, Elliot W. Veras, Solange M. Yongyu, Mei |
| author_facet | Dietzgen, Ralf Georg Bejerman, Nicolas Esteban Yongyu, Mei Jee, Charmaine Lim Jing Chabi-Jesus, Camila Freitas-Astúa, Juliana Veras, Solange M. Kitajima, Elliot W. |
| author_sort | Dietzgen, Ralf Georg |
| collection | INTA Digital |
| description | We identified a novel plant rhabdovirus infecting native joá (Solanum aculeatissimum) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The virus could be graft-transmitted to healthy joá and tomato plants but was not mechanically transmissible. RT-PCR using degenerate plant rhabdovirus L gene primers yielded an amplicon from extracted total RNA, the sequence of which was similar to those of alphanucleorhabdoviruses. Based on close sequence matches, especially with the type member potato yellow dwarf virus (PYDV), we adopted a degenerate-primer-walking strategy towards both genome ends. The complete genome of joá yellow blotch-associated virus (JYBaV) is comprised of 12,965 nucleotides, is less than 75% identical to that of its closest relative PYDV, and clusters with PYDV and other alphanucleorhabdoviruses in L protein phylogenetic trees, suggesting that it should be taxonomically classified in a new species in the genus Alphanucleorhabdovirus, family Rhabdoviridae. The genome organization of JYBaV is typical of the ‘PYDV-like’ subgroup of alphanucleorhabdoviruses, with seven genes (N-X-P-Y-M-G-L) separated by conserved intergenic regions and flanked by partly complementary 3' leader and 5' trailer regions. |
| format | info:ar-repo/semantics/artículo |
| id | INTA23082 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA230822025-07-18T14:26:58Z Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil Dietzgen, Ralf Georg Bejerman, Nicolas Esteban Yongyu, Mei Jee, Charmaine Lim Jing Chabi-Jesus, Camila Freitas-Astúa, Juliana Veras, Solange M. Kitajima, Elliot W. Solanum Plant Viruses Rhabdoviridae Plant Rhabdoviruses Brazil Virus de las Plantas Rhabdovirus de las Plantas Brasil Solanum aculeatissimum We identified a novel plant rhabdovirus infecting native joá (Solanum aculeatissimum) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The virus could be graft-transmitted to healthy joá and tomato plants but was not mechanically transmissible. RT-PCR using degenerate plant rhabdovirus L gene primers yielded an amplicon from extracted total RNA, the sequence of which was similar to those of alphanucleorhabdoviruses. Based on close sequence matches, especially with the type member potato yellow dwarf virus (PYDV), we adopted a degenerate-primer-walking strategy towards both genome ends. The complete genome of joá yellow blotch-associated virus (JYBaV) is comprised of 12,965 nucleotides, is less than 75% identical to that of its closest relative PYDV, and clusters with PYDV and other alphanucleorhabdoviruses in L protein phylogenetic trees, suggesting that it should be taxonomically classified in a new species in the genus Alphanucleorhabdovirus, family Rhabdoviridae. The genome organization of JYBaV is typical of the ‘PYDV-like’ subgroup of alphanucleorhabdoviruses, with seven genes (N-X-P-Y-M-G-L) separated by conserved intergenic regions and flanked by partly complementary 3' leader and 5' trailer regions. Instituto de Patología Vegetal Fil: Dietzgen, Ralf G. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia Fil: Dietzgen, Ralf G. University of Queensland. School of Chemistry and Molecular Biosciences; Australia Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Yongyu, Mei. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia Fil: Yongyu, Mei. University of Queensland. School of Chemistry and Molecular Biosciences; Australia Fil: Jee, Charmaine Lim Jing. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia Fil: Jee, Charmaine Lim Jing. University of Queensland. School of Chemistry and Molecular Biosciences; Australia Fil: Chabi-Jesus, Camila. Instituto Biológico (São Paulo); Brasil Fil: Freitas-Astúa, Juliana. Instituto Biológico (São Paulo); Brasil Fil: Freitas-Astúa, Juliana. Embrapa Cassava and Fruits; Brasil Fil: Veras, Solange M. Universidade Federal do Amazonas. Departamento de Ciências Fundamentais e Desenvolvimento Agrícola; Brasil Fil: Kitajima, Elliot W.. Universidade de Sao Paulo. Escuela Superior de Agricultura Luiz de Queiroz; Brasil 2025-07-18T14:20:06Z 2025-07-18T14:20:06Z 2021 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23082 https://link.springer.com/article/10.1007/s00705-021-05040-y 0304-8608 1432-8798 https://doi.org/10.1007/s00705-021-05040-y eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Archives of Virology 166 : 1615-1622. (2021) |
| spellingShingle | Solanum Plant Viruses Rhabdoviridae Plant Rhabdoviruses Brazil Virus de las Plantas Rhabdovirus de las Plantas Brasil Solanum aculeatissimum Dietzgen, Ralf Georg Bejerman, Nicolas Esteban Yongyu, Mei Jee, Charmaine Lim Jing Chabi-Jesus, Camila Freitas-Astúa, Juliana Veras, Solange M. Kitajima, Elliot W. Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil |
| title | Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil |
| title_full | Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil |
| title_fullStr | Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil |
| title_full_unstemmed | Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil |
| title_short | Joá yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil |
| title_sort | joa yellow blotch associated virus a new alphanucleorhabdovirus from a wild solanaceous plant in brazil |
| topic | Solanum Plant Viruses Rhabdoviridae Plant Rhabdoviruses Brazil Virus de las Plantas Rhabdovirus de las Plantas Brasil Solanum aculeatissimum |
| url | http://hdl.handle.net/20.500.12123/23082 https://link.springer.com/article/10.1007/s00705-021-05040-y https://doi.org/10.1007/s00705-021-05040-y |
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