Urinary bladder lesions of bovine enzootic hematuria in Argentina

Bovine enzootic hematuria (BEH) is characterized by chronic hematuria and neoplasms in the urinary bladder. From 2013 to 2016, 64 bovine urinary bladders were collected at a slaughterhouse in Rosario de Lerma, Salta, Argentina. The animals came from endemic areas where the infestation of ferns (Pter...

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Autores principales: Aguirre Castro, Laura Sabrina, Barbeito, Claudio Gustavo, Medina, Diego Martín Nicolás, Colque Caro, Luis Adrián, Micheloud, Juan Francisco
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/23062
https://www.sciencedirect.com/science/article/abs/pii/S0034528825002711
https://doi.org/10.1016/j.rvsc.2025.105797
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author Aguirre Castro, Laura Sabrina
Barbeito, Claudio Gustavo
Medina, Diego Martín Nicolás
Colque Caro, Luis Adrián
Micheloud, Juan Francisco
author_browse Aguirre Castro, Laura Sabrina
Barbeito, Claudio Gustavo
Colque Caro, Luis Adrián
Medina, Diego Martín Nicolás
Micheloud, Juan Francisco
author_facet Aguirre Castro, Laura Sabrina
Barbeito, Claudio Gustavo
Medina, Diego Martín Nicolás
Colque Caro, Luis Adrián
Micheloud, Juan Francisco
author_sort Aguirre Castro, Laura Sabrina
collection INTA Digital
description Bovine enzootic hematuria (BEH) is characterized by chronic hematuria and neoplasms in the urinary bladder. From 2013 to 2016, 64 bovine urinary bladders were collected at a slaughterhouse in Rosario de Lerma, Salta, Argentina. The animals came from endemic areas where the infestation of ferns (Pteridium spp., Pteris spp.) in pastures is high. Histopathological and immunohistochemical analyses were performed. A significant increase in bladder thickness of positive cases compared to negative controls was observed (BEH+:7.5 ± 2 vs. BEH-: 3.9 ± 0.8). Samples were classified according to the severity of the thickening as normal, mildly, moderately, or severely thickened. Non-neoplastic lesions (epithelial, vascular, and inflammatory) and neoplasms of epithelial and vascular origin with varying degrees of severity were observed. Pancytokeratin AE1-AE3 and VEGF C1 antibodies were used to diagnose and confirm the origin of epithelial and vascular neoplastic cells. Proliferation indices were determined based on PCNA expression. Confirmation of the diagnosis of animals affected by this disease in the region. It was demonstrated that proliferative changes justified the thickening of the urinary bladders and may be related to the future development of certain lesions. Even though, this finding is important in the early stages of the disease and can be used as a parameter for monitoring the disease. Lesions observed in BEH are remarkable and demonstrate the complexity of the interactions in bladder tissue during carcinogenesis. The development of lesions serves as a valuable model for investigating neoplastic lesions in the bladder, with potential implications for human bladder cancer.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA230622025-07-17T13:32:43Z Urinary bladder lesions of bovine enzootic hematuria in Argentina Aguirre Castro, Laura Sabrina Barbeito, Claudio Gustavo Medina, Diego Martín Nicolás Colque Caro, Luis Adrián Micheloud, Juan Francisco Ganado Bovino Enfermedades del Tracto Urinario Hematuria Plantas Tóxicas Argentina Cattle Urinary Tract Diseases Haematuria Poisonous Plants Bovine enzootic hematuria (BEH) is characterized by chronic hematuria and neoplasms in the urinary bladder. From 2013 to 2016, 64 bovine urinary bladders were collected at a slaughterhouse in Rosario de Lerma, Salta, Argentina. The animals came from endemic areas where the infestation of ferns (Pteridium spp., Pteris spp.) in pastures is high. Histopathological and immunohistochemical analyses were performed. A significant increase in bladder thickness of positive cases compared to negative controls was observed (BEH+:7.5 ± 2 vs. BEH-: 3.9 ± 0.8). Samples were classified according to the severity of the thickening as normal, mildly, moderately, or severely thickened. Non-neoplastic lesions (epithelial, vascular, and inflammatory) and neoplasms of epithelial and vascular origin with varying degrees of severity were observed. Pancytokeratin AE1-AE3 and VEGF C1 antibodies were used to diagnose and confirm the origin of epithelial and vascular neoplastic cells. Proliferation indices were determined based on PCNA expression. Confirmation of the diagnosis of animals affected by this disease in the region. It was demonstrated that proliferative changes justified the thickening of the urinary bladders and may be related to the future development of certain lesions. Even though, this finding is important in the early stages of the disease and can be used as a parameter for monitoring the disease. Lesions observed in BEH are remarkable and demonstrate the complexity of the interactions in bladder tissue during carcinogenesis. The development of lesions serves as a valuable model for investigating neoplastic lesions in the bladder, with potential implications for human bladder cancer. Instituto de Investigación Animal del Chaco Semiárido Fil: Aguirre Castro, Laura Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Aguirre Castro, Laura Sabrina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina Fil: Aguirre Castro, Laura Sabrina. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina Fil: Barbeito, Claudio G. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Histología y Embriología Descriptiva, Experimental y Comparada; Argentina Fil: Medina, Diego Martín Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Medina, Diego Martín Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina Fil: Medina, Diego Martín Nicolás. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina Fil: Colque Caro, Luis Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Colque Caro, Luis Adrián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina Fil: Micheloud, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina Fil: Micheloud, Juan Francisco. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina 2025-07-17T13:28:51Z 2025-07-17T13:28:51Z 2025-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/23062 https://www.sciencedirect.com/science/article/abs/pii/S0034528825002711 0034-5288 1532-2661 https://doi.org/10.1016/j.rvsc.2025.105797 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Research in Veterinary Science 193 : 105797. (September 2025)
spellingShingle Ganado Bovino
Enfermedades del Tracto Urinario
Hematuria
Plantas Tóxicas
Argentina
Cattle
Urinary Tract Diseases
Haematuria
Poisonous Plants
Aguirre Castro, Laura Sabrina
Barbeito, Claudio Gustavo
Medina, Diego Martín Nicolás
Colque Caro, Luis Adrián
Micheloud, Juan Francisco
Urinary bladder lesions of bovine enzootic hematuria in Argentina
title Urinary bladder lesions of bovine enzootic hematuria in Argentina
title_full Urinary bladder lesions of bovine enzootic hematuria in Argentina
title_fullStr Urinary bladder lesions of bovine enzootic hematuria in Argentina
title_full_unstemmed Urinary bladder lesions of bovine enzootic hematuria in Argentina
title_short Urinary bladder lesions of bovine enzootic hematuria in Argentina
title_sort urinary bladder lesions of bovine enzootic hematuria in argentina
topic Ganado Bovino
Enfermedades del Tracto Urinario
Hematuria
Plantas Tóxicas
Argentina
Cattle
Urinary Tract Diseases
Haematuria
Poisonous Plants
url http://hdl.handle.net/20.500.12123/23062
https://www.sciencedirect.com/science/article/abs/pii/S0034528825002711
https://doi.org/10.1016/j.rvsc.2025.105797
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