Genome characterization of Enterococcus lactis strain isolated from bovine subclinical mastitis

Environmental bacteria, such as streptococci and enterococci, play an important role in bovine mastitis. This study aims to characterize the genome sequence of Enterococcus spp. isolated from a case of subclinical mastitis. Genomic DNA was isolated and sequenced using Illumina and Oxford Nanopore pl...

Descripción completa

Detalles Bibliográficos
Autores principales: Cerioli, María Florencia, Fernandez, Franco Daniel, Moliva, Melina Vanesa, Serral, Federico, Fernandez Do Porto, Darío, Reinoso, Elina B.
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/22932
https://www.sciencedirect.com/science/article/abs/pii/S0882401025005716
https://doi.org/10.1016/j.micpath.2025.107846
_version_ 1855487036404793344
author Cerioli, María Florencia
Fernandez, Franco Daniel
Moliva, Melina Vanesa
Serral, Federico
Fernandez Do Porto, Darío
Reinoso, Elina B.
author_browse Cerioli, María Florencia
Fernandez Do Porto, Darío
Fernandez, Franco Daniel
Moliva, Melina Vanesa
Reinoso, Elina B.
Serral, Federico
author_facet Cerioli, María Florencia
Fernandez, Franco Daniel
Moliva, Melina Vanesa
Serral, Federico
Fernandez Do Porto, Darío
Reinoso, Elina B.
author_sort Cerioli, María Florencia
collection INTA Digital
description Environmental bacteria, such as streptococci and enterococci, play an important role in bovine mastitis. This study aims to characterize the genome sequence of Enterococcus spp. isolated from a case of subclinical mastitis. Genomic DNA was isolated and sequenced using Illumina and Oxford Nanopore platforms. Final genome was assembled in a single circular chromosome and one plasmid comprising 2.556.630 bp (GC% 38.60) and 150.514 bp (GC 36 %), respectively. Phylogeny confirmed that strain SU-B46 is closely related to E. lactis reference genomes. The alignment confirmed the presence of E. lactis. Additionally, the genomic analysis identified twelve potential virulence genes related to adhesion, immune evasion and biofilm formation among others, but none were found in the plasmid. The strain exhibited resistance to aminoglycosides, macrolides and glycopeptides. This study presents the first report of an E. lactis strain isolated from a subclinical mastitis case in Argentina, broadening the geographical and host range of this pathogen. These findings suggest the possible involvement of E. lactis as an etiological agent of bovine mastitis. Further studies are needed to understand the significance of this agent, warranting additional investigation to ensure its optimal management, especially within the dairy industry.
format Artículo
id INTA22932
institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2025
publishDateRange 2025
publishDateSort 2025
publisher Elsevier
publisherStr Elsevier
record_format dspace
spelling INTA229322025-07-07T12:47:53Z Genome characterization of Enterococcus lactis strain isolated from bovine subclinical mastitis Cerioli, María Florencia Fernandez, Franco Daniel Moliva, Melina Vanesa Serral, Federico Fernandez Do Porto, Darío Reinoso, Elina B. Bovine Mastitis Antimicrobial Resistance Genomic Features Mastítis Bovina Resistencia a los Antimicrobianos Enterococcus Características Genómicas Enterococcus lactis Genomic Characterization Virulence Genes Environmental bacteria, such as streptococci and enterococci, play an important role in bovine mastitis. This study aims to characterize the genome sequence of Enterococcus spp. isolated from a case of subclinical mastitis. Genomic DNA was isolated and sequenced using Illumina and Oxford Nanopore platforms. Final genome was assembled in a single circular chromosome and one plasmid comprising 2.556.630 bp (GC% 38.60) and 150.514 bp (GC 36 %), respectively. Phylogeny confirmed that strain SU-B46 is closely related to E. lactis reference genomes. The alignment confirmed the presence of E. lactis. Additionally, the genomic analysis identified twelve potential virulence genes related to adhesion, immune evasion and biofilm formation among others, but none were found in the plasmid. The strain exhibited resistance to aminoglycosides, macrolides and glycopeptides. This study presents the first report of an E. lactis strain isolated from a subclinical mastitis case in Argentina, broadening the geographical and host range of this pathogen. These findings suggest the possible involvement of E. lactis as an etiological agent of bovine mastitis. Further studies are needed to understand the significance of this agent, warranting additional investigation to ensure its optimal management, especially within the dairy industry. Instituto de Patología Vegetal Fil: Cerioli, María F. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico - Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Cerioli, María F. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y de la Salud (INBIAS); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y de la Salud (INBIAS); Argentina Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Moliva, Melina V. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico - Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Moliva, Melina V. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y de la Salud (INBIAS); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y de la Salud (INBIAS); Argentina Fil: Serral, Federico. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Cálculo; Argentina Fil: Serral, Federico. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina Fil: Fernandez Do Porto, Darío. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Cálculo; Argentina Fil: Fernandez Do Porto, Darío. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina Fil: Reinoso, Elina B. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico - Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Reinoso, Elina B. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y de la Salud (INBIAS); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y de la Salud (INBIAS); Argentina 2025-07-07T12:38:15Z 2025-07-07T12:38:15Z 2025-06-27 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22932 https://www.sciencedirect.com/science/article/abs/pii/S0882401025005716 0882-4010 1096-1208 https://doi.org/10.1016/j.micpath.2025.107846 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Microbial Pathogenesis 206 : 107846 (September 2025)
spellingShingle Bovine Mastitis
Antimicrobial Resistance
Genomic Features
Mastítis Bovina
Resistencia a los Antimicrobianos
Enterococcus
Características Genómicas
Enterococcus lactis
Genomic Characterization
Virulence Genes
Cerioli, María Florencia
Fernandez, Franco Daniel
Moliva, Melina Vanesa
Serral, Federico
Fernandez Do Porto, Darío
Reinoso, Elina B.
Genome characterization of Enterococcus lactis strain isolated from bovine subclinical mastitis
title Genome characterization of Enterococcus lactis strain isolated from bovine subclinical mastitis
title_full Genome characterization of Enterococcus lactis strain isolated from bovine subclinical mastitis
title_fullStr Genome characterization of Enterococcus lactis strain isolated from bovine subclinical mastitis
title_full_unstemmed Genome characterization of Enterococcus lactis strain isolated from bovine subclinical mastitis
title_short Genome characterization of Enterococcus lactis strain isolated from bovine subclinical mastitis
title_sort genome characterization of enterococcus lactis strain isolated from bovine subclinical mastitis
topic Bovine Mastitis
Antimicrobial Resistance
Genomic Features
Mastítis Bovina
Resistencia a los Antimicrobianos
Enterococcus
Características Genómicas
Enterococcus lactis
Genomic Characterization
Virulence Genes
url http://hdl.handle.net/20.500.12123/22932
https://www.sciencedirect.com/science/article/abs/pii/S0882401025005716
https://doi.org/10.1016/j.micpath.2025.107846
work_keys_str_mv AT ceriolimariaflorencia genomecharacterizationofenterococcuslactisstrainisolatedfrombovinesubclinicalmastitis
AT fernandezfrancodaniel genomecharacterizationofenterococcuslactisstrainisolatedfrombovinesubclinicalmastitis
AT molivamelinavanesa genomecharacterizationofenterococcuslactisstrainisolatedfrombovinesubclinicalmastitis
AT serralfederico genomecharacterizationofenterococcuslactisstrainisolatedfrombovinesubclinicalmastitis
AT fernandezdoportodario genomecharacterizationofenterococcuslactisstrainisolatedfrombovinesubclinicalmastitis
AT reinosoelinab genomecharacterizationofenterococcuslactisstrainisolatedfrombovinesubclinicalmastitis