Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds
Thiamethoxam (TMX) is a neonicotinoid insecticide that is widely used for seed treatment in a variety of crops. Farmland birds can be exposed to TMX by consuming treated seeds remaining on the soil surface due to either spills or failed implantation during sowing. In the environment, TMX can be meta...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2025
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/22884 https://www.sciencedirect.com/science/article/pii/S0269749125010802 https://doi.org/10.1016/j.envpol.2025.126707 |
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| author | Fernández Vizcaíno, Elena Poliserpi, Maria Belen Ortiz- Santaliestra, Manuel E. Mougeot, Francois Mateo, Rafael Camarero, Pablo R. Brodeur, Celine Marie |
| author_browse | Brodeur, Celine Marie Camarero, Pablo R. Fernández Vizcaíno, Elena Mateo, Rafael Mougeot, Francois Ortiz- Santaliestra, Manuel E. Poliserpi, Maria Belen |
| author_facet | Fernández Vizcaíno, Elena Poliserpi, Maria Belen Ortiz- Santaliestra, Manuel E. Mougeot, Francois Mateo, Rafael Camarero, Pablo R. Brodeur, Celine Marie |
| author_sort | Fernández Vizcaíno, Elena |
| collection | INTA Digital |
| description | Thiamethoxam (TMX) is a neonicotinoid insecticide that is widely used for seed treatment in a variety of crops. Farmland birds can be exposed to TMX by consuming treated seeds remaining on the soil surface due to either spills or failed implantation during sowing. In the environment, TMX can be metabolized into clothianidin (CLO), another neonicotinoid insecticide that is also toxic to birds. The goal of the present study was to evaluate the feasibility of a non-invasive approach consisting in the analysis of fecal samples to detect recent exposure of wild birds to TMX and CLO. To achieve this, 16 grayish baywings (Agelaioides badius) were exposed for 21 days to seeds coated with four different concentrations of TMX: 0 (control), 0.027 (low), 0.33 (medium), and 4.3 (high) g TMX/kg of seed. These concentrations are within the approved range for seed coating with TMX in typical crops of the Pampa Region in Argentina, where baywings are common. Seed consumption was monitored over 24 h and fecal samples were collected on day 1, 2, 3, 4, 7, and 21 of exposure. Finally, concentrations TMX and CLO were determined in collected feces through LC-MS/MS. Fecal concentrations of both TMX and CLO showed a significant dependence on the treatment dose (p = 0.006). Average concentrations of TMX and CLO were respectively 63, 689, and 2363 ng/g and 75, 735 and 2040 ng/g for low, medium and high dose groups. These results demonstrate that fecal analysis is a reliable non-invasive method for assessing recent bird exposure to TMX. The results also confirm that TMX is metabolized into CLO, and suggest a saturation of the metabolic pathway at high doses. This study supports the use of fecal analysis for pesticide exposure estimation in field monitoring studies of wild birds. |
| format | Artículo |
| id | INTA22884 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA228842025-07-03T13:54:03Z Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds Fernández Vizcaíno, Elena Poliserpi, Maria Belen Ortiz- Santaliestra, Manuel E. Mougeot, Francois Mateo, Rafael Camarero, Pablo R. Brodeur, Celine Marie Faeces Pesticide Exposure Birds Neonicotinoid Insecticides Thiamethoxam Heces Exposición al Pesticida Pájaros Insecticida Neonicotinoide Tiametoxam Monitoreo no Invasivo Semillas Tratadas Non-invasive Monitoring Treated Seeds Thiamethoxam (TMX) is a neonicotinoid insecticide that is widely used for seed treatment in a variety of crops. Farmland birds can be exposed to TMX by consuming treated seeds remaining on the soil surface due to either spills or failed implantation during sowing. In the environment, TMX can be metabolized into clothianidin (CLO), another neonicotinoid insecticide that is also toxic to birds. The goal of the present study was to evaluate the feasibility of a non-invasive approach consisting in the analysis of fecal samples to detect recent exposure of wild birds to TMX and CLO. To achieve this, 16 grayish baywings (Agelaioides badius) were exposed for 21 days to seeds coated with four different concentrations of TMX: 0 (control), 0.027 (low), 0.33 (medium), and 4.3 (high) g TMX/kg of seed. These concentrations are within the approved range for seed coating with TMX in typical crops of the Pampa Region in Argentina, where baywings are common. Seed consumption was monitored over 24 h and fecal samples were collected on day 1, 2, 3, 4, 7, and 21 of exposure. Finally, concentrations TMX and CLO were determined in collected feces through LC-MS/MS. Fecal concentrations of both TMX and CLO showed a significant dependence on the treatment dose (p = 0.006). Average concentrations of TMX and CLO were respectively 63, 689, and 2363 ng/g and 75, 735 and 2040 ng/g for low, medium and high dose groups. These results demonstrate that fecal analysis is a reliable non-invasive method for assessing recent bird exposure to TMX. The results also confirm that TMX is metabolized into CLO, and suggest a saturation of the metabolic pathway at high doses. This study supports the use of fecal analysis for pesticide exposure estimation in field monitoring studies of wild birds. Instituto de Recursos Biológicos Fil: Fernández-Vizcaíno, Elena. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España Fil: Poliserpi, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Ortiz-Santaliestra, Manuel E. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España Fil: Mougeot, Francois. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España Fil: Mateo, Rafael. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España. CSIC. Institute for Environmental Assessment and Water Research (IDAEA); España Fil: Camarero, Pablo R. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España Fil: Brodeur, Julie C. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Brodeur, Julie C. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-07-03T13:42:15Z 2025-07-03T13:42:15Z 2025-06-19 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22884 https://www.sciencedirect.com/science/article/pii/S0269749125010802 0269-7491 1873-6424 https://doi.org/10.1016/j.envpol.2025.126707 eng info:eu-repograntAgreement/INTA/2023-PD-L03-I094, Gestión ambiental de los agroquímicos: manejo, mitigación y remediación info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Environmental Pollution 382 : 126707. (October 2025) |
| spellingShingle | Faeces Pesticide Exposure Birds Neonicotinoid Insecticides Thiamethoxam Heces Exposición al Pesticida Pájaros Insecticida Neonicotinoide Tiametoxam Monitoreo no Invasivo Semillas Tratadas Non-invasive Monitoring Treated Seeds Fernández Vizcaíno, Elena Poliserpi, Maria Belen Ortiz- Santaliestra, Manuel E. Mougeot, Francois Mateo, Rafael Camarero, Pablo R. Brodeur, Celine Marie Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds |
| title | Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds |
| title_full | Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds |
| title_fullStr | Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds |
| title_full_unstemmed | Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds |
| title_short | Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds |
| title_sort | monitoring approach to detect recent exposure to thiamethoxam treated seeds in birds |
| topic | Faeces Pesticide Exposure Birds Neonicotinoid Insecticides Thiamethoxam Heces Exposición al Pesticida Pájaros Insecticida Neonicotinoide Tiametoxam Monitoreo no Invasivo Semillas Tratadas Non-invasive Monitoring Treated Seeds |
| url | http://hdl.handle.net/20.500.12123/22884 https://www.sciencedirect.com/science/article/pii/S0269749125010802 https://doi.org/10.1016/j.envpol.2025.126707 |
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