Humoral and cell-mediated immune response against the Mce2B (Rv0590/Mb0605) cell-wall protein of Mycobacterium bovis
Bovine tuberculosis is a zoonotic disease with global distribution. Improved diagnosis is essential thus, research into new diagnostic reagents is valuable. The Mce2B recombinant protein was evaluated as an inducer of immune response The research involved naturally infected cattle with different imm...
| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/22881 https://www.sciencedirect.com/science/article/abs/pii/S0165242725000583 https://doi.org/10.1016/j.vetimm.2025.110938 |
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| author | Ferrara Muñiz, Ximena Encinas, Micaela Marques Da Silva, Wanderson Garbaccio, Sergio Gabriel Garro, Carlos Javier Sammarruco, Romina Ayelén Carignano, Hugo Adrian Bianco, María Veronica Cataldi, Angel Adrian Zumarraga, Martin Jose Eirin, Maria Emilia |
| author_browse | Bianco, María Veronica Carignano, Hugo Adrian Cataldi, Angel Adrian Eirin, Maria Emilia Encinas, Micaela Ferrara Muñiz, Ximena Garbaccio, Sergio Gabriel Garro, Carlos Javier Marques Da Silva, Wanderson Sammarruco, Romina Ayelén Zumarraga, Martin Jose |
| author_facet | Ferrara Muñiz, Ximena Encinas, Micaela Marques Da Silva, Wanderson Garbaccio, Sergio Gabriel Garro, Carlos Javier Sammarruco, Romina Ayelén Carignano, Hugo Adrian Bianco, María Veronica Cataldi, Angel Adrian Zumarraga, Martin Jose Eirin, Maria Emilia |
| author_sort | Ferrara Muñiz, Ximena |
| collection | INTA Digital |
| description | Bovine tuberculosis is a zoonotic disease with global distribution. Improved diagnosis is essential thus, research into new diagnostic reagents is valuable. The Mce2B recombinant protein was evaluated as an inducer of immune response The research involved naturally infected cattle with different immunological profiles. Moderate homology (≥ 40 %) between Mce2B of M. bovis and homologous proteins in non-tuberculous mycobacteria was corroborated, as well as the presence of epitopes restricted by the bovine leucocyte antigen class II. Despite this prediction, cell-mediated responses to Mce2B were undetectable in caudal fold tuberculin skin test (CF-TST) positive and non-infected animals. In CF-TST false-negative cattle, a minimal cell-mediated response was observed (5 %; IC 95 %: 0.13–24.9), lower than that elicited by PPDB (35 %; IC 95 %: 15,4–59,2) (p = 0.046) but identical to the recombinant Fusion Protein including ESAT-6, CFP-10, EspC antigens (5 %; IC 95 %: 34.9–96.8). Marginal humoral response (33.3 %; IC 95 %: 4.3–77.7) was observed in the non-infected group. These findings demonstrate that the Mce2B protein is not a suitable antigen for bovine tuberculosis diagnosis. |
| format | Artículo |
| id | INTA22881 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA228812025-07-03T13:20:23Z Humoral and cell-mediated immune response against the Mce2B (Rv0590/Mb0605) cell-wall protein of Mycobacterium bovis Ferrara Muñiz, Ximena Encinas, Micaela Marques Da Silva, Wanderson Garbaccio, Sergio Gabriel Garro, Carlos Javier Sammarruco, Romina Ayelén Carignano, Hugo Adrian Bianco, María Veronica Cataldi, Angel Adrian Zumarraga, Martin Jose Eirin, Maria Emilia Bovine Tuberculosis Serology Recombinant Antigens Immune Response Humoral Immunity In vitro Tuberculosis Bovina Serología Antígenos Recombinantes Respuesta Inmunológica Mycobacterium bovis Inmunidad Humoral Bovine tuberculosis is a zoonotic disease with global distribution. Improved diagnosis is essential thus, research into new diagnostic reagents is valuable. The Mce2B recombinant protein was evaluated as an inducer of immune response The research involved naturally infected cattle with different immunological profiles. Moderate homology (≥ 40 %) between Mce2B of M. bovis and homologous proteins in non-tuberculous mycobacteria was corroborated, as well as the presence of epitopes restricted by the bovine leucocyte antigen class II. Despite this prediction, cell-mediated responses to Mce2B were undetectable in caudal fold tuberculin skin test (CF-TST) positive and non-infected animals. In CF-TST false-negative cattle, a minimal cell-mediated response was observed (5 %; IC 95 %: 0.13–24.9), lower than that elicited by PPDB (35 %; IC 95 %: 15,4–59,2) (p = 0.046) but identical to the recombinant Fusion Protein including ESAT-6, CFP-10, EspC antigens (5 %; IC 95 %: 34.9–96.8). Marginal humoral response (33.3 %; IC 95 %: 4.3–77.7) was observed in the non-infected group. These findings demonstrate that the Mce2B protein is not a suitable antigen for bovine tuberculosis diagnosis. Instituto de Biotecnología Fil: Ferrara Muñiz, Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ferrara Muñiz, Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Encinas, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Encinas, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Marques Da Silva, Wanderson. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Garbaccio, Sergio Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Garro, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sammarruco, Romina Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Sammarruco, Romina Ayelén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carignano, Hugo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bianco, María Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Eirin, Maria Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Eirin, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-07-03T13:12:34Z 2025-07-03T13:12:34Z 2025-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22881 https://www.sciencedirect.com/science/article/abs/pii/S0165242725000583 0165-2427 https://doi.org/10.1016/j.vetimm.2025.110938 eng info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Immunology and Immunopathology 284 : 110938 (June 2025) |
| spellingShingle | Bovine Tuberculosis Serology Recombinant Antigens Immune Response Humoral Immunity In vitro Tuberculosis Bovina Serología Antígenos Recombinantes Respuesta Inmunológica Mycobacterium bovis Inmunidad Humoral Ferrara Muñiz, Ximena Encinas, Micaela Marques Da Silva, Wanderson Garbaccio, Sergio Gabriel Garro, Carlos Javier Sammarruco, Romina Ayelén Carignano, Hugo Adrian Bianco, María Veronica Cataldi, Angel Adrian Zumarraga, Martin Jose Eirin, Maria Emilia Humoral and cell-mediated immune response against the Mce2B (Rv0590/Mb0605) cell-wall protein of Mycobacterium bovis |
| title | Humoral and cell-mediated immune response against the Mce2B (Rv0590/Mb0605) cell-wall protein of Mycobacterium bovis |
| title_full | Humoral and cell-mediated immune response against the Mce2B (Rv0590/Mb0605) cell-wall protein of Mycobacterium bovis |
| title_fullStr | Humoral and cell-mediated immune response against the Mce2B (Rv0590/Mb0605) cell-wall protein of Mycobacterium bovis |
| title_full_unstemmed | Humoral and cell-mediated immune response against the Mce2B (Rv0590/Mb0605) cell-wall protein of Mycobacterium bovis |
| title_short | Humoral and cell-mediated immune response against the Mce2B (Rv0590/Mb0605) cell-wall protein of Mycobacterium bovis |
| title_sort | humoral and cell mediated immune response against the mce2b rv0590 mb0605 cell wall protein of mycobacterium bovis |
| topic | Bovine Tuberculosis Serology Recombinant Antigens Immune Response Humoral Immunity In vitro Tuberculosis Bovina Serología Antígenos Recombinantes Respuesta Inmunológica Mycobacterium bovis Inmunidad Humoral |
| url | http://hdl.handle.net/20.500.12123/22881 https://www.sciencedirect.com/science/article/abs/pii/S0165242725000583 https://doi.org/10.1016/j.vetimm.2025.110938 |
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