An Integrated Physical Map of the Cultivated Hot Chili Pepper, Capsicum baccatum var. Pendulum
Capsicum baccatum var. Pendulum is the most frequently cultivated chili pepper in South America. This taxon is an outstanding source for breeding programs and genetic improvement in the genus, since its traits can be introgressed into C. annuum and related species to generate viable hybrids. In this...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://www.fspublishers.org/published_papers/30787_..pdf http://hdl.handle.net/20.500.12123/2283 |
| _version_ | 1855483027577110528 |
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| author | Aguilera, Patricia Mabel Debat, Humberto Julio Grabiele, Mauro |
| author_browse | Aguilera, Patricia Mabel Debat, Humberto Julio Grabiele, Mauro |
| author_facet | Aguilera, Patricia Mabel Debat, Humberto Julio Grabiele, Mauro |
| author_sort | Aguilera, Patricia Mabel |
| collection | INTA Digital |
| description | Capsicum baccatum var. Pendulum is the most frequently cultivated chili pepper in South America. This taxon is an outstanding source for breeding programs and genetic improvement in the genus, since its traits can be introgressed into C. annuum and related species to generate viable hybrids. In this study, the chromosome complement of C. baccatum var. Pendulum 2n = 24 was deeply characterized through a sequential combination of conventional and molecular cytogenetics approaches comprising Ag-NOR staining, heterochromatic fluorescence C-DAPI and CMA/DA/DAPI bandings and fluorescent in situ hybridization (FISH) of Capsicum-derived 5S and 18S-25S ribosomal genes and spacers probes. The
attained markers were systematically integrated into a physical map which allows the identification of each individual chromosome. This is the first integrated physical map of C. baccatum var. Pendulum, which in conjunction with reported
genetic linkage maps of Capsicum, would be useful for future high-density genetic mapping and whole-genome studies in C. baccatum and related taxa, as well as in breeding and genetic improvement programs in chili peppers |
| format | Artículo |
| id | INTA2283 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA22832018-04-23T13:20:22Z An Integrated Physical Map of the Cultivated Hot Chili Pepper, Capsicum baccatum var. Pendulum Aguilera, Patricia Mabel Debat, Humberto Julio Grabiele, Mauro Capsicum baccatum Guindilla Genética Citogenética Cromosomas Chillies Genetics Cytogenetics Chromosomes Capsicum pendulum Capsicum baccatum var. Pendulum is the most frequently cultivated chili pepper in South America. This taxon is an outstanding source for breeding programs and genetic improvement in the genus, since its traits can be introgressed into C. annuum and related species to generate viable hybrids. In this study, the chromosome complement of C. baccatum var. Pendulum 2n = 24 was deeply characterized through a sequential combination of conventional and molecular cytogenetics approaches comprising Ag-NOR staining, heterochromatic fluorescence C-DAPI and CMA/DA/DAPI bandings and fluorescent in situ hybridization (FISH) of Capsicum-derived 5S and 18S-25S ribosomal genes and spacers probes. The attained markers were systematically integrated into a physical map which allows the identification of each individual chromosome. This is the first integrated physical map of C. baccatum var. Pendulum, which in conjunction with reported genetic linkage maps of Capsicum, would be useful for future high-density genetic mapping and whole-genome studies in C. baccatum and related taxa, as well as in breeding and genetic improvement programs in chili peppers Instituto de Patología Vegetal Fil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnología de Misiones; Argentina Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnología de Misiones; Argentina 2018-04-23T13:18:23Z 2018-04-23T13:18:23Z 2017 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://www.fspublishers.org/published_papers/30787_..pdf http://hdl.handle.net/20.500.12123/2283 1560-8530 1814-9596 10.17957/IJAB/15.0303 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf International Journal of Agriculture and Biology 19 (3) : 465‒469 (2017) |
| spellingShingle | Capsicum baccatum Guindilla Genética Citogenética Cromosomas Chillies Genetics Cytogenetics Chromosomes Capsicum pendulum Aguilera, Patricia Mabel Debat, Humberto Julio Grabiele, Mauro An Integrated Physical Map of the Cultivated Hot Chili Pepper, Capsicum baccatum var. Pendulum |
| title | An Integrated Physical Map of the Cultivated Hot Chili Pepper, Capsicum baccatum var. Pendulum |
| title_full | An Integrated Physical Map of the Cultivated Hot Chili Pepper, Capsicum baccatum var. Pendulum |
| title_fullStr | An Integrated Physical Map of the Cultivated Hot Chili Pepper, Capsicum baccatum var. Pendulum |
| title_full_unstemmed | An Integrated Physical Map of the Cultivated Hot Chili Pepper, Capsicum baccatum var. Pendulum |
| title_short | An Integrated Physical Map of the Cultivated Hot Chili Pepper, Capsicum baccatum var. Pendulum |
| title_sort | integrated physical map of the cultivated hot chili pepper capsicum baccatum var pendulum |
| topic | Capsicum baccatum Guindilla Genética Citogenética Cromosomas Chillies Genetics Cytogenetics Chromosomes Capsicum pendulum |
| url | http://www.fspublishers.org/published_papers/30787_..pdf http://hdl.handle.net/20.500.12123/2283 |
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