Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection

RNA interference (RNAi) appears as a promising strategy to control virus replication. While the antiviral power of short-hairpin RNAs or small-interfering RNAs against FMDV has been demonstrated widely, safer RNAi effectors such as artificial microRNAs (amiRs) have not been evaluated extensively. In...

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Autores principales: Gismondi, Maria Ines, Ortiz, Xoana P., Curra, Anabella Paola, Asurmendi, Sebastian, Taboga, Oscar Alberto
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: 2018
Materias:
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0166093413005181#!
http://hdl.handle.net/20.500.12123/2277
https://doi.org/10.1016/j.jviromet.2013.12.016
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author Gismondi, Maria Ines
Ortiz, Xoana P.
Curra, Anabella Paola
Asurmendi, Sebastian
Taboga, Oscar Alberto
author_browse Asurmendi, Sebastian
Curra, Anabella Paola
Gismondi, Maria Ines
Ortiz, Xoana P.
Taboga, Oscar Alberto
author_facet Gismondi, Maria Ines
Ortiz, Xoana P.
Curra, Anabella Paola
Asurmendi, Sebastian
Taboga, Oscar Alberto
author_sort Gismondi, Maria Ines
collection INTA Digital
description RNA interference (RNAi) appears as a promising strategy to control virus replication. While the antiviral power of short-hairpin RNAs or small-interfering RNAs against FMDV has been demonstrated widely, safer RNAi effectors such as artificial microRNAs (amiRs) have not been evaluated extensively. In this work, transgenic monoclonal cell lines constitutively expressing different amiRs targeting FMDV 3D-coding region or 3′UTR were established. Certain cell lines showed an effective, sequence-specific amiR-mediated silencing activity that was accomplished by degradation of the target mRNA, as demonstrated in co-transfection experiments of reporter genes fused to FMDV target sequences. However, FMDV replication in these amiR-expressing cells was affected barely. Experiments aimed at elucidating the cause of RNAi failure demonstrated limited accessibility of the targeted region in the molecular environment of the viral RNA. Since RNAi is mediated by large-dimension silencing complexes containing the siRNA and not simply by a linear oligonucleotide, we propose that target selection should consider not only the local RNA structure but also the global conformation of target RNA.
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spelling INTA22772018-06-28T17:59:48Z Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection Gismondi, Maria Ines Ortiz, Xoana P. Curra, Anabella Paola Asurmendi, Sebastian Taboga, Oscar Alberto ARN Genética Virus de las Plantas Viricidas RNA Genetics Plant Viruses Antiviral Agents MicroRNA Ácido Ribonucléico RNA interference (RNAi) appears as a promising strategy to control virus replication. While the antiviral power of short-hairpin RNAs or small-interfering RNAs against FMDV has been demonstrated widely, safer RNAi effectors such as artificial microRNAs (amiRs) have not been evaluated extensively. In this work, transgenic monoclonal cell lines constitutively expressing different amiRs targeting FMDV 3D-coding region or 3′UTR were established. Certain cell lines showed an effective, sequence-specific amiR-mediated silencing activity that was accomplished by degradation of the target mRNA, as demonstrated in co-transfection experiments of reporter genes fused to FMDV target sequences. However, FMDV replication in these amiR-expressing cells was affected barely. Experiments aimed at elucidating the cause of RNAi failure demonstrated limited accessibility of the targeted region in the molecular environment of the viral RNA. Since RNAi is mediated by large-dimension silencing complexes containing the siRNA and not simply by a linear oligonucleotide, we propose that target selection should consider not only the local RNA structure but also the global conformation of target RNA. Instituto de Biotecnología Fil: Gismondi, Maria Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Ortiz, Xoana P. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Curra, Anabella Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2018-04-19T13:27:48Z 2018-04-19T13:27:48Z 2014-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0166093413005181#! http://hdl.handle.net/20.500.12123/2277 0166-0934 https://doi.org/10.1016/j.jviromet.2013.12.016 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of virological methods 199 : 1-10. (April 2014)
spellingShingle ARN
Genética
Virus de las Plantas
Viricidas
RNA
Genetics
Plant Viruses
Antiviral Agents
MicroRNA
Ácido Ribonucléico
Gismondi, Maria Ines
Ortiz, Xoana P.
Curra, Anabella Paola
Asurmendi, Sebastian
Taboga, Oscar Alberto
Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
title Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
title_full Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
title_fullStr Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
title_full_unstemmed Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
title_short Artificial microRNAs as antiviral strategy to FMDV: structural implications of target selection
title_sort artificial micrornas as antiviral strategy to fmdv structural implications of target selection
topic ARN
Genética
Virus de las Plantas
Viricidas
RNA
Genetics
Plant Viruses
Antiviral Agents
MicroRNA
Ácido Ribonucléico
url https://www.sciencedirect.com/science/article/pii/S0166093413005181#!
http://hdl.handle.net/20.500.12123/2277
https://doi.org/10.1016/j.jviromet.2013.12.016
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