Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity
Arbuscular mycorrhizal fungi (AMF) and Trichoderma spp. (T) are known as plant-beneficial fungi effective against root-knot nematodes, but their interactions in the rhizosphere are not well understood. This study examined how Meloidogyne javanica influences AMF colonization and community diversity a...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/22672 https://www.mdpi.com/1424-2818/17/5/334 https://doi.org/10.3390/d17050334 |
| _version_ | 1855038618923433984 |
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| author | Covacevich, Fernanda Fernandez Gnecco, Gabriela Amancay Consolo, Verónica Burges, Pablo Calo, Gonzalo Mondino, Eduardo Ariel |
| author_browse | Burges, Pablo Calo, Gonzalo Consolo, Verónica Covacevich, Fernanda Fernandez Gnecco, Gabriela Amancay Mondino, Eduardo Ariel |
| author_facet | Covacevich, Fernanda Fernandez Gnecco, Gabriela Amancay Consolo, Verónica Burges, Pablo Calo, Gonzalo Mondino, Eduardo Ariel |
| author_sort | Covacevich, Fernanda |
| collection | INTA Digital |
| description | Arbuscular mycorrhizal fungi (AMF) and Trichoderma spp. (T) are known as plant-beneficial fungi effective against root-knot nematodes, but their interactions in the rhizosphere are not well understood. This study examined how Meloidogyne javanica influences AMF colonization and community diversity at the root-soil interface of tomato plants. A 60-day growth chamber experiment was conducted with tomato plants grown in non-sterile agricultural soil, either infected or not with M. javanica, that received a single inoculation with AMF or Trichoderma (strains T363 or TJ15), combined AMF + T inoculations, or no inoculation (Control). Both single and combined inoculations significantly reduced root galls, eggs, and soil nematode larvae. An AMF community analysis via single-strand conformation polymorphism of the D1 region of 28S rDNA gene (Glomeraceae family) revealed that M. javanica decreased AMF diversity and altered community structure, in plants single-inoculated with AMF. However, a combined inoculation with Trichoderma appears to prevent this reduction and maintain AMF diversity. While M. javanica reduced root mycorrhizal colonization, it did not affect Trichoderma abundance. These results suggest that Trichoderma may be more resilient to nematode infection, helping stabilize AMF communities and enhance biocontrol. Thus, combining AMF and Trichoderma inoculations could better preserve root health and improve biological control effectiveness against M. javanica. |
| format | info:ar-repo/semantics/artículo |
| id | INTA22672 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA226722025-06-13T10:09:07Z Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity Covacevich, Fernanda Fernandez Gnecco, Gabriela Amancay Consolo, Verónica Burges, Pablo Calo, Gonzalo Mondino, Eduardo Ariel Biodiversidad Suelo Nematodos Parásitos Inoculación Control Biológico Biodiversity Soil Nematodes Parasites Mycorrhizae Trichoderma Inoculation Biological Control Arbuscular mycorrhizal fungi (AMF) and Trichoderma spp. (T) are known as plant-beneficial fungi effective against root-knot nematodes, but their interactions in the rhizosphere are not well understood. This study examined how Meloidogyne javanica influences AMF colonization and community diversity at the root-soil interface of tomato plants. A 60-day growth chamber experiment was conducted with tomato plants grown in non-sterile agricultural soil, either infected or not with M. javanica, that received a single inoculation with AMF or Trichoderma (strains T363 or TJ15), combined AMF + T inoculations, or no inoculation (Control). Both single and combined inoculations significantly reduced root galls, eggs, and soil nematode larvae. An AMF community analysis via single-strand conformation polymorphism of the D1 region of 28S rDNA gene (Glomeraceae family) revealed that M. javanica decreased AMF diversity and altered community structure, in plants single-inoculated with AMF. However, a combined inoculation with Trichoderma appears to prevent this reduction and maintain AMF diversity. While M. javanica reduced root mycorrhizal colonization, it did not affect Trichoderma abundance. These results suggest that Trichoderma may be more resilient to nematode infection, helping stabilize AMF communities and enhance biocontrol. Thus, combining AMF and Trichoderma inoculations could better preserve root health and improve biological control effectiveness against M. javanica. EEA Balcarce Fil: Covacevich, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; Argentina Fil: Fernandez Gnecco, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; Argentina Fil: Consolo, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; Argentina Fil: Burges, Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Calo, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Instituto de Biodiversidad y Biotecnología; Argentina Fil: Mondino, Eduardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina 2025-06-13T10:01:27Z 2025-06-13T10:01:27Z 2025-05-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22672 https://www.mdpi.com/1424-2818/17/5/334 1424-2818 https://doi.org/10.3390/d17050334 eng info:eu-repograntAgreement/INTA/2023-PD-L03-I093, Fertilidad química, física y biológica del suelo: estrategias para mitigar y restaurar procesos de degradación info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Diversity 17 (5) : 334 (May 2025) |
| spellingShingle | Biodiversidad Suelo Nematodos Parásitos Inoculación Control Biológico Biodiversity Soil Nematodes Parasites Mycorrhizae Trichoderma Inoculation Biological Control Covacevich, Fernanda Fernandez Gnecco, Gabriela Amancay Consolo, Verónica Burges, Pablo Calo, Gonzalo Mondino, Eduardo Ariel Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity |
| title | Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity |
| title_full | Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity |
| title_fullStr | Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity |
| title_full_unstemmed | Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity |
| title_short | Combined Soil Inoculation with Mycorrhizae and Trichoderma Alleviates Nematode-Induced Decline in Mycorrhizal Diversity |
| title_sort | combined soil inoculation with mycorrhizae and trichoderma alleviates nematode induced decline in mycorrhizal diversity |
| topic | Biodiversidad Suelo Nematodos Parásitos Inoculación Control Biológico Biodiversity Soil Nematodes Parasites Mycorrhizae Trichoderma Inoculation Biological Control |
| url | http://hdl.handle.net/20.500.12123/22672 https://www.mdpi.com/1424-2818/17/5/334 https://doi.org/10.3390/d17050334 |
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