Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea
BACKGROUND: Botrytis cinerea is a relevant fungal pathogen in the production of blueberries, which is managed mainly with fungicides. However, the intensive usage has led to the emergence of resistant strains. The use of microbial biocontrol agents is a sustainable strategy to overcome limitations a...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Wiley
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/22657 https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8964 https://doi.org/10.1002/ps.8964 |
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| author | Ferreira, Flavia Vanina Rivadeneira, Maria Fernanda Bello, Fernando Giudici, Vanesa Noelia Musumeci, Matías Alejandro |
| author_browse | Bello, Fernando Ferreira, Flavia Vanina Giudici, Vanesa Noelia Musumeci, Matías Alejandro Rivadeneira, Maria Fernanda |
| author_facet | Ferreira, Flavia Vanina Rivadeneira, Maria Fernanda Bello, Fernando Giudici, Vanesa Noelia Musumeci, Matías Alejandro |
| author_sort | Ferreira, Flavia Vanina |
| collection | INTA Digital |
| description | BACKGROUND: Botrytis cinerea is a relevant fungal pathogen in the production of blueberries, which is managed mainly with fungicides. However, the intensive usage has led to the emergence of resistant strains. The use of microbial biocontrol agents is a sustainable strategy to overcome limitations associated with fungicides. The objectives of this work were to know the capability of the endophytic bacterium Burkholderia contaminans 128 to inhibit different phytopathogens in vitro and to prevent B. cinerea rot in blueberry fruits.
RESULTS: Burkholderia contaminans 128 able to grow on filaments of Trichoderma citrinoviride was isolated from roots of blueberry shrub. The bacterial cell-free supernatant (Bc128 CFS) inhibited efficiently the mycelia growth and conidia germination of B. cinerea and other important pathogens affecting blueberry such as Alternaria sp., Colletotrichum gloeosporioides and Fusarium graminearum. HPLC–MS/MS analyses of Bc128 CFS confirmed production of occidiofungins and pyrrolnitrin, compounds with fungicide activity but different inhibition mechanisms. Qualitative and quantitative HPLC-MS/MS analyses determined the presence of indole-3-acetic acid in Bc128 CFS, a phytohormone involved in plant-growth promotion. Bc128 CFS inhibited completely the natural occurrence of conidia of Alternaria sp., Colletotrichum sp. and Pestalotiopsis sp. and reduced significantly the incidence of B. cinerea conidia in blueberry flowers. Bc128 CFS decreased the virulence of B. cinerea in blueberries approximately 90%. Bc128 CFS was more efficient to control B. cinerea in preventive than curative treatments and did not affect fruit quality.
CONCLUSION: B. contaminans 128 provides direct and indirect antibiosis mechanisms useful to prevent rots in blueberries caused by B. cinerea. |
| format | info:ar-repo/semantics/artículo |
| id | INTA22657 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA226572025-06-12T12:43:11Z Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea Ferreira, Flavia Vanina Rivadeneira, Maria Fernanda Bello, Fernando Giudici, Vanesa Noelia Musumeci, Matías Alejandro Arándano Control Biológico Botrytis cinerea Agentes de Control Biológico Blueberries Burkholderiaceae Biological Control Biological Control Agents Burkholderia contaminans BACKGROUND: Botrytis cinerea is a relevant fungal pathogen in the production of blueberries, which is managed mainly with fungicides. However, the intensive usage has led to the emergence of resistant strains. The use of microbial biocontrol agents is a sustainable strategy to overcome limitations associated with fungicides. The objectives of this work were to know the capability of the endophytic bacterium Burkholderia contaminans 128 to inhibit different phytopathogens in vitro and to prevent B. cinerea rot in blueberry fruits. RESULTS: Burkholderia contaminans 128 able to grow on filaments of Trichoderma citrinoviride was isolated from roots of blueberry shrub. The bacterial cell-free supernatant (Bc128 CFS) inhibited efficiently the mycelia growth and conidia germination of B. cinerea and other important pathogens affecting blueberry such as Alternaria sp., Colletotrichum gloeosporioides and Fusarium graminearum. HPLC–MS/MS analyses of Bc128 CFS confirmed production of occidiofungins and pyrrolnitrin, compounds with fungicide activity but different inhibition mechanisms. Qualitative and quantitative HPLC-MS/MS analyses determined the presence of indole-3-acetic acid in Bc128 CFS, a phytohormone involved in plant-growth promotion. Bc128 CFS inhibited completely the natural occurrence of conidia of Alternaria sp., Colletotrichum sp. and Pestalotiopsis sp. and reduced significantly the incidence of B. cinerea conidia in blueberry flowers. Bc128 CFS decreased the virulence of B. cinerea in blueberries approximately 90%. Bc128 CFS was more efficient to control B. cinerea in preventive than curative treatments and did not affect fruit quality. CONCLUSION: B. contaminans 128 provides direct and indirect antibiosis mechanisms useful to prevent rots in blueberries caused by B. cinerea. EEA Concordia Fil: Ferreira, Flavia Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina Fil: Rivadeneira, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Giudici, Vanesa. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina Fil: Musumeci, Matías Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina Fil: Musumeci, Matías Alejandro. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina 2025-06-12T12:40:25Z 2025-06-12T12:40:25Z 2025-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22657 https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8964 1526-498X 1526-4998 https://doi.org/10.1002/ps.8964 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Pest Management Science : 1-15. (First published: 11 June 2025) |
| spellingShingle | Arándano Control Biológico Botrytis cinerea Agentes de Control Biológico Blueberries Burkholderiaceae Biological Control Biological Control Agents Burkholderia contaminans Ferreira, Flavia Vanina Rivadeneira, Maria Fernanda Bello, Fernando Giudici, Vanesa Noelia Musumeci, Matías Alejandro Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea |
| title | Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea |
| title_full | Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea |
| title_fullStr | Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea |
| title_full_unstemmed | Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea |
| title_short | Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea |
| title_sort | burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by botrytis cinerea |
| topic | Arándano Control Biológico Botrytis cinerea Agentes de Control Biológico Blueberries Burkholderiaceae Biological Control Biological Control Agents Burkholderia contaminans |
| url | http://hdl.handle.net/20.500.12123/22657 https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8964 https://doi.org/10.1002/ps.8964 |
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