Engineering a commercial soybean inoculant to efficiently degrade glyphosate
Nitrogen-fixing rhizobia possess the well-characterized phn operon, which encodes the C–P lyase enzymatic complex responsible for degrading phosphonates such as glyphosate. However, glyphosate exerts a bacteriostatic effect on these endosymbionts, hindering the activation of this natural degradation...
| Autores principales: | , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/22616 https://www.sciencedirect.com/science/article/abs/pii/S2452219825000941 https://doi.org/10.1016/j.rhisph.2025.101109 |
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| author | Brambilla, Silvina Maricel Pascuan, Cecilia Gabriela Frare, Romina Alejandra Liebrenz, Karen Ivana Pesquero, Natalia Ruiz, María Juana Soto, Gabriela Cynthia Cristos, Diego Sebastian Ayub, Nicolás Daniel |
| author_browse | Ayub, Nicolás Daniel Brambilla, Silvina Maricel Cristos, Diego Sebastian Frare, Romina Alejandra Liebrenz, Karen Ivana Pascuan, Cecilia Gabriela Pesquero, Natalia Ruiz, María Juana Soto, Gabriela Cynthia |
| author_facet | Brambilla, Silvina Maricel Pascuan, Cecilia Gabriela Frare, Romina Alejandra Liebrenz, Karen Ivana Pesquero, Natalia Ruiz, María Juana Soto, Gabriela Cynthia Cristos, Diego Sebastian Ayub, Nicolás Daniel |
| author_sort | Brambilla, Silvina Maricel |
| collection | INTA Digital |
| description | Nitrogen-fixing rhizobia possess the well-characterized phn operon, which encodes the C–P lyase enzymatic complex responsible for degrading phosphonates such as glyphosate. However, glyphosate exerts a bacteriostatic effect on these endosymbionts, hindering the activation of this natural degradation pathway and thereby limiting its multiple potential benefits within glyphosate-tolerant cropping systems. In this study, we characterized a mutant derived from the commercial inoculant Bradyrhizobium japonicum E109, carrying a single-nucleotide substitution causing a Ser100Ala change in lactate dehydrogenase. This mutation enhances the detoxification of methylglyoxal, a toxic byproduct induced by glyphosate, and, importantly, enables the mutant strain to efficiently degrade glyphosate and improve crop productivity. Since inoculation is routine in soybean (Glycine max (L.) Merr.) production, this strategy adds no additional cost, benefiting farmer profitability as well as the environment and public health. |
| format | info:ar-repo/semantics/artículo |
| id | INTA22616 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA226162025-06-10T15:11:14Z Engineering a commercial soybean inoculant to efficiently degrade glyphosate Brambilla, Silvina Maricel Pascuan, Cecilia Gabriela Frare, Romina Alejandra Liebrenz, Karen Ivana Pesquero, Natalia Ruiz, María Juana Soto, Gabriela Cynthia Cristos, Diego Sebastian Ayub, Nicolás Daniel Glycine max Glifosato Soja Fijación del Nitrógeno Rhizobiaceae Glyphosate Soybeans Nitrogen Fixation Nitrogen-fixing rhizobia possess the well-characterized phn operon, which encodes the C–P lyase enzymatic complex responsible for degrading phosphonates such as glyphosate. However, glyphosate exerts a bacteriostatic effect on these endosymbionts, hindering the activation of this natural degradation pathway and thereby limiting its multiple potential benefits within glyphosate-tolerant cropping systems. In this study, we characterized a mutant derived from the commercial inoculant Bradyrhizobium japonicum E109, carrying a single-nucleotide substitution causing a Ser100Ala change in lactate dehydrogenase. This mutation enhances the detoxification of methylglyoxal, a toxic byproduct induced by glyphosate, and, importantly, enables the mutant strain to efficiently degrade glyphosate and improve crop productivity. Since inoculation is routine in soybean (Glycine max (L.) Merr.) production, this strategy adds no additional cost, benefiting farmer profitability as well as the environment and public health. Instituto de Biotecnología Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Brambilla, Silvina Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Pascuan, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Frare, Romina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Liebrenz, Karen Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Liebrenz, Karen Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Liebrenz, Karen Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Pesquero, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina Fil: Pesquero, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ruiz, María Juana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina Fil: Ruiz, María Juana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Cristos, Diego Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina Fil: Cristos, Diego Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2025-06-10T15:04:20Z 2025-06-10T15:04:20Z 2025-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22616 https://www.sciencedirect.com/science/article/abs/pii/S2452219825000941 2452-2198 https://doi.org/10.1016/j.rhisph.2025.101109 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Rhizosphere 34 : 101109 (June 2025) |
| spellingShingle | Glycine max Glifosato Soja Fijación del Nitrógeno Rhizobiaceae Glyphosate Soybeans Nitrogen Fixation Brambilla, Silvina Maricel Pascuan, Cecilia Gabriela Frare, Romina Alejandra Liebrenz, Karen Ivana Pesquero, Natalia Ruiz, María Juana Soto, Gabriela Cynthia Cristos, Diego Sebastian Ayub, Nicolás Daniel Engineering a commercial soybean inoculant to efficiently degrade glyphosate |
| title | Engineering a commercial soybean inoculant to efficiently degrade glyphosate |
| title_full | Engineering a commercial soybean inoculant to efficiently degrade glyphosate |
| title_fullStr | Engineering a commercial soybean inoculant to efficiently degrade glyphosate |
| title_full_unstemmed | Engineering a commercial soybean inoculant to efficiently degrade glyphosate |
| title_short | Engineering a commercial soybean inoculant to efficiently degrade glyphosate |
| title_sort | engineering a commercial soybean inoculant to efficiently degrade glyphosate |
| topic | Glycine max Glifosato Soja Fijación del Nitrógeno Rhizobiaceae Glyphosate Soybeans Nitrogen Fixation |
| url | http://hdl.handle.net/20.500.12123/22616 https://www.sciencedirect.com/science/article/abs/pii/S2452219825000941 https://doi.org/10.1016/j.rhisph.2025.101109 |
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