A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs
The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using pr...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2025
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/22576 https://link.springer.com/article/10.1007/s10493-025-01026-6 https://doi.org/10.1007/s10493-025-01026-6 |
| _version_ | 1855486964942241792 |
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| author | Obiegala, Anna Król, Nina Heyse, Lara M.I. Pfeffer, Martin Montini, Martina Nava, Santiago Sebastian, Patrick |
| author_browse | Heyse, Lara M.I. Król, Nina Montini, Martina Nava, Santiago Obiegala, Anna Pfeffer, Martin Sebastian, Patrick |
| author_facet | Obiegala, Anna Król, Nina Heyse, Lara M.I. Pfeffer, Martin Montini, Martina Nava, Santiago Sebastian, Patrick |
| author_sort | Obiegala, Anna |
| collection | INTA Digital |
| description | The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3–85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96–16.3 mg) and subsequent molting (78.3–100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy. |
| format | Artículo |
| id | INTA22576 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA225762025-06-09T11:05:30Z A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs Obiegala, Anna Król, Nina Heyse, Lara M.I. Pfeffer, Martin Montini, Martina Nava, Santiago Sebastian, Patrick Amblyomma Feeding Systems Ticks Laboratory Animals Tick-borne Diseases Sistema de Alimentación Garrapatas Animal de Laboratorio Enfermedad Transmitida por Garrapatas Amblyomma sculptum The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3–85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96–16.3 mg) and subsequent molting (78.3–100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy. EEA Rafaela Fil: Obiegala, Anna. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania Fil: Król, Nina. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania Fil: Heyse, Lara M.I. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania Fil: Pfeffer, Martin. University of Leipzig. Faculty of Veterinary Medicine. Institute of Animal Hygiene and Veterinary Public Health; Alemania Fil: Montini, Martina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina 2025-06-09T11:02:29Z 2025-06-09T11:02:29Z 2025-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22576 https://link.springer.com/article/10.1007/s10493-025-01026-6 0168-8162 1572-9702 https://doi.org/10.1007/s10493-025-01026-6 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Experimental and Applied Acarology 94 : article number 57. (2025) |
| spellingShingle | Amblyomma Feeding Systems Ticks Laboratory Animals Tick-borne Diseases Sistema de Alimentación Garrapatas Animal de Laboratorio Enfermedad Transmitida por Garrapatas Amblyomma sculptum Obiegala, Anna Król, Nina Heyse, Lara M.I. Pfeffer, Martin Montini, Martina Nava, Santiago Sebastian, Patrick A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs |
| title | A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs |
| title_full | A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs |
| title_fullStr | A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs |
| title_full_unstemmed | A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs |
| title_short | A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs |
| title_sort | silicon membrane based artificial feeding system for amblyomma sculptum nymphs |
| topic | Amblyomma Feeding Systems Ticks Laboratory Animals Tick-borne Diseases Sistema de Alimentación Garrapatas Animal de Laboratorio Enfermedad Transmitida por Garrapatas Amblyomma sculptum |
| url | http://hdl.handle.net/20.500.12123/22576 https://link.springer.com/article/10.1007/s10493-025-01026-6 https://doi.org/10.1007/s10493-025-01026-6 |
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