In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba)
Neltuma alba (Formerly Prosopis alba), known as ‘algarrobo blanco,’ is a native species of great biological, productive, and cultural importance in the phytogeographic regions of the Gran Chaco and the Espinal. However, it faces severe conservation threats due to deforestation and unsustainable mana...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/22572 https://link.springer.com/article/10.1007/s11240-025-03107-1 https://doi.org/10.1007/s11240-025-03107-1 |
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| author | Carloni, Edgardo José Rodriguez, Milena Nahir Verga, Anibal Grunberg, Karina |
| author_browse | Carloni, Edgardo José Grunberg, Karina Rodriguez, Milena Nahir Verga, Anibal |
| author_facet | Carloni, Edgardo José Rodriguez, Milena Nahir Verga, Anibal Grunberg, Karina |
| author_sort | Carloni, Edgardo José |
| collection | INTA Digital |
| description | Neltuma alba (Formerly Prosopis alba), known as ‘algarrobo blanco,’ is a native species of great biological, productive, and cultural importance in the phytogeographic regions of the Gran Chaco and the Espinal. However, it faces severe conservation threats due to deforestation and unsustainable management of native forests. In this context, vegetative propagation through in vitro micrografting emerges as an innovative strategy for preserving forest genetic resources. This study aimed to develop a vegetative propagation system based on in vitro micrografting and evaluate its feasibility in adult N. alba trees. Twelve-day-old in vitro-grown seedlings were used as rootstocks (etiolated vs. non-etiolated). Lateral buds were collected from current-year branches of adult trees, and the micrografts were cultured on MS medium supplemented with 0.1 mg L−1 of either BAP, IBA, or GA₃ individually. A Control treatment without growth regulators was also included. Apical micrografts sprouted from the second week onwards. Anatomical studies confirmed vascular reconnection between the scion and rootstock. Sprouting frequency was influenced by the season and hypocotyl type, although growth regulators showed no significant effect. During summer, the probability of contamination was higher than in spring. Ex-vitro acclimatization showed no differences between rootstock types, but IBA treatment increased survival rates. These results demonstrate that in vitro micrografting is a novel and promising tool for the vegetative propagation of adult N. alba trees. |
| format | info:ar-repo/semantics/artículo |
| id | INTA22572 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA225722025-06-09T10:13:12Z In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba) Carloni, Edgardo José Rodriguez, Milena Nahir Verga, Anibal Grunberg, Karina Vegetative Propagation Forestry Propagación Vegetativa Ciencia forestal Prosopis alba Plant Tissue Culture Micrografting Rejuvenation Rhizogenesis Algarrobo blanco Neltuma alba Neltuma alba (Formerly Prosopis alba), known as ‘algarrobo blanco,’ is a native species of great biological, productive, and cultural importance in the phytogeographic regions of the Gran Chaco and the Espinal. However, it faces severe conservation threats due to deforestation and unsustainable management of native forests. In this context, vegetative propagation through in vitro micrografting emerges as an innovative strategy for preserving forest genetic resources. This study aimed to develop a vegetative propagation system based on in vitro micrografting and evaluate its feasibility in adult N. alba trees. Twelve-day-old in vitro-grown seedlings were used as rootstocks (etiolated vs. non-etiolated). Lateral buds were collected from current-year branches of adult trees, and the micrografts were cultured on MS medium supplemented with 0.1 mg L−1 of either BAP, IBA, or GA₃ individually. A Control treatment without growth regulators was also included. Apical micrografts sprouted from the second week onwards. Anatomical studies confirmed vascular reconnection between the scion and rootstock. Sprouting frequency was influenced by the season and hypocotyl type, although growth regulators showed no significant effect. During summer, the probability of contamination was higher than in spring. Ex-vitro acclimatization showed no differences between rootstock types, but IBA treatment increased survival rates. These results demonstrate that in vitro micrografting is a novel and promising tool for the vegetative propagation of adult N. alba trees. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Carloni, Edgardo José. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Rodriguez, Milena Nahir. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Rodriguez, Milena Nahir.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Verga, Anibal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Rioja. Agencia de Extensión Rural La Rioja; Argentina Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Grunberg, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina 2025-06-09T10:03:27Z 2025-06-09T10:03:27Z 2025-05-28 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22572 https://link.springer.com/article/10.1007/s11240-025-03107-1 0167-6857 1573-5044 https://doi.org/10.1007/s11240-025-03107-1 eng info:eu-repograntAgreement/INTA/2019-RIST-E4-I091-001, Multiplicación de propágulos de sanidad controlada info:eu-repograntAgreement/INTA/2023-PE-L01-I067, Mejoramiento genético y silvicultura de plantaciones para la producción sostenible de productos forestales para distintos destinos industriales info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Plant Cell, Tissue and Organ Culture (PCTOC) 161 : 77. (Published: 28 May 2025) |
| spellingShingle | Vegetative Propagation Forestry Propagación Vegetativa Ciencia forestal Prosopis alba Plant Tissue Culture Micrografting Rejuvenation Rhizogenesis Algarrobo blanco Neltuma alba Carloni, Edgardo José Rodriguez, Milena Nahir Verga, Anibal Grunberg, Karina In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba) |
| title | In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba) |
| title_full | In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba) |
| title_fullStr | In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba) |
| title_full_unstemmed | In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba) |
| title_short | In vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco (Neltuma alba) |
| title_sort | in vitro micrografting as an innovative strategy for the vegetative propagation of algarrobo blanco neltuma alba |
| topic | Vegetative Propagation Forestry Propagación Vegetativa Ciencia forestal Prosopis alba Plant Tissue Culture Micrografting Rejuvenation Rhizogenesis Algarrobo blanco Neltuma alba |
| url | http://hdl.handle.net/20.500.12123/22572 https://link.springer.com/article/10.1007/s11240-025-03107-1 https://doi.org/10.1007/s11240-025-03107-1 |
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