Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae)
Purpose: To locate transient chromosome aberrations on a selected pepper cultivar and determine the tracing efficiency of different cytogenetic methods. Materials and methods: Seeds from Capsicum baccatum var. pendulum cultivar ‘Cayenne’ were treated with an acute dose of X-rays (300 Gy) and chromos...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | https://www.tandfonline.com/doi/abs/10.3109/09553002.2013.825064 http://hdl.handle.net/20.500.12123/2242 https://doi.org/10.3109/09553002.2013.825064 |
| _version_ | 1855034928853417984 |
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| author | Scaldaferro, Marisel Analía Grabiele, Mauro Seijo, Jose Guillermo Debat, Humberto Julio Romero, María Victoria Ducasse, Daniel Adrian Prina, Alberto Raul Moscone, Eduardo Alberto |
| author_browse | Debat, Humberto Julio Ducasse, Daniel Adrian Grabiele, Mauro Moscone, Eduardo Alberto Prina, Alberto Raul Romero, María Victoria Scaldaferro, Marisel Analía Seijo, Jose Guillermo |
| author_facet | Scaldaferro, Marisel Analía Grabiele, Mauro Seijo, Jose Guillermo Debat, Humberto Julio Romero, María Victoria Ducasse, Daniel Adrian Prina, Alberto Raul Moscone, Eduardo Alberto |
| author_sort | Scaldaferro, Marisel Analía |
| collection | INTA Digital |
| description | Purpose: To locate transient chromosome aberrations on a selected pepper cultivar and determine the tracing efficiency of different cytogenetic methods. Materials and methods: Seeds from Capsicum baccatum var. pendulum cultivar ‘Cayenne’ were treated with an acute dose of X-rays (300 Gy) and chromosome aberrations were analysed by different cytogenetic methods [Feulgen, silver staining for nucleolus organizer regions (silver positive nucleolus organizing regions or AgNOR), fluorescent banding, fluorescence in situ hybridization (FISH) and meiotic analysis]. Results: A rearranged chromosome carrying two nucleolus organizing regions (NOR) induced by ionizing radiation was detected in the cultivar, with the occurrence of a small reciprocal exchange between a chromosome of pair no. 1 and another chromosome of pair no. 3, both carrying active NOR in short arms and associated chromomycin A positive/diamidino-phenylindole negative (CMA+/DAPI−) heterochromatin. Meiotic analysis showed a quadrivalent configuration, confirming a reciprocal translocation between two chromosomes. Conclusions: The use of X-rays in Capsicum allowed us to develop and identify a pepper line with structural rearrangements between two NOR-carrying chromosomes. We postulate that all the cytological techniques employed in this research were efficient in the search for chromosome aberrations. Particularly, Feulgen and AgNOR were the most suitable in those cases of transient rearrangements, whereas fluorescent banding and FISH were appropriate for intransitive ones. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2242 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA22422018-10-26T18:54:58Z Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae) Scaldaferro, Marisel Analía Grabiele, Mauro Seijo, Jose Guillermo Debat, Humberto Julio Romero, María Victoria Ducasse, Daniel Adrian Prina, Alberto Raul Moscone, Eduardo Alberto Capsicum baccatum Guindilla Cromosomas Citogenética Radiación Ionizante Chillies Chromosomes Cytogenetics Ionizing Radiation Capsicum pendulum Purpose: To locate transient chromosome aberrations on a selected pepper cultivar and determine the tracing efficiency of different cytogenetic methods. Materials and methods: Seeds from Capsicum baccatum var. pendulum cultivar ‘Cayenne’ were treated with an acute dose of X-rays (300 Gy) and chromosome aberrations were analysed by different cytogenetic methods [Feulgen, silver staining for nucleolus organizer regions (silver positive nucleolus organizing regions or AgNOR), fluorescent banding, fluorescence in situ hybridization (FISH) and meiotic analysis]. Results: A rearranged chromosome carrying two nucleolus organizing regions (NOR) induced by ionizing radiation was detected in the cultivar, with the occurrence of a small reciprocal exchange between a chromosome of pair no. 1 and another chromosome of pair no. 3, both carrying active NOR in short arms and associated chromomycin A positive/diamidino-phenylindole negative (CMA+/DAPI−) heterochromatin. Meiotic analysis showed a quadrivalent configuration, confirming a reciprocal translocation between two chromosomes. Conclusions: The use of X-rays in Capsicum allowed us to develop and identify a pepper line with structural rearrangements between two NOR-carrying chromosomes. We postulate that all the cytological techniques employed in this research were efficient in the search for chromosome aberrations. Particularly, Feulgen and AgNOR were the most suitable in those cases of transient rearrangements, whereas fluorescent banding and FISH were appropriate for intransitive ones. Instituto de Patología Vegetal Instituto de Genética Fil: Scaldaferro, Marisel Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste; Argentina Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Romero, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Moscone, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina 2018-04-13T13:35:48Z 2018-04-13T13:35:48Z 2014 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion https://www.tandfonline.com/doi/abs/10.3109/09553002.2013.825064 http://hdl.handle.net/20.500.12123/2242 0955-3002 1362-3095 https://doi.org/10.3109/09553002.2013.825064 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf International Journal of Radiation Biology 90 (1) : 104-112 (2014) |
| spellingShingle | Capsicum baccatum Guindilla Cromosomas Citogenética Radiación Ionizante Chillies Chromosomes Cytogenetics Ionizing Radiation Capsicum pendulum Scaldaferro, Marisel Analía Grabiele, Mauro Seijo, Jose Guillermo Debat, Humberto Julio Romero, María Victoria Ducasse, Daniel Adrian Prina, Alberto Raul Moscone, Eduardo Alberto Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae) |
| title | Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae) |
| title_full | Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae) |
| title_fullStr | Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae) |
| title_full_unstemmed | Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae) |
| title_short | Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae) |
| title_sort | efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line capsicum baccatum var pendulum solanaceae |
| topic | Capsicum baccatum Guindilla Cromosomas Citogenética Radiación Ionizante Chillies Chromosomes Cytogenetics Ionizing Radiation Capsicum pendulum |
| url | https://www.tandfonline.com/doi/abs/10.3109/09553002.2013.825064 http://hdl.handle.net/20.500.12123/2242 https://doi.org/10.3109/09553002.2013.825064 |
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