The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.)
We present the first report of a virus infecting the subtropical tree crop yerba mate (Ilex paraguariensis St. Hil.). Total RNA purification, followed by next-generation sequencing, transcripts assembly and annotation, resulted in the identification of a new endornavirus species infecting yerba mate...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
|
| Materias: | |
| Acceso en línea: | https://link.springer.com/article/10.1007%2Fs11262-014-1096-2 http://hdl.handle.net/20.500.12123/2241 https://doi.org/10.1007/s11262-014-1096-2 |
| _version_ | 1855483020130123776 |
|---|---|
| author | Debat, Humberto Julio Grabiele, Mauro Aguilera, Patricia Mabel Bubillo, Rosana Elizabeth Zapata, Pedro Darío Marti, Dardo Andrea Ducasse, Daniel Adrian |
| author_browse | Aguilera, Patricia Mabel Bubillo, Rosana Elizabeth Debat, Humberto Julio Ducasse, Daniel Adrian Grabiele, Mauro Marti, Dardo Andrea Zapata, Pedro Darío |
| author_facet | Debat, Humberto Julio Grabiele, Mauro Aguilera, Patricia Mabel Bubillo, Rosana Elizabeth Zapata, Pedro Darío Marti, Dardo Andrea Ducasse, Daniel Adrian |
| author_sort | Debat, Humberto Julio |
| collection | INTA Digital |
| description | We present the first report of a virus infecting the subtropical tree crop yerba mate (Ilex paraguariensis St. Hil.). Total RNA purification, followed by next-generation sequencing, transcripts assembly and annotation, resulted in the identification of a new endornavirus species infecting yerba mate. The complete sequence of the linear dsRNA viral genome is 13,954-nt long, contains a single 13,743 nt ORF, and presents a 149 nt 5′UTR and a 61 nt 3′UTR. The predicted ORF encodes a 4,581 aa polypeptide with a UDP-glucose glycosyl-transferase, a capsular polysaccharide synthesis protein, and a RNA-dependent RNA polymerase domain. The name yerba mate endornavirus is proposed for the identified virus. Due to the intriguing peculiarities of this virus family, and the complete lack of the yerba mate virus literature, we consider that the information reported here will be helpful in leading to a new and needed attention to this important topic and crop. |
| format | Artículo |
| id | INTA2241 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA22412018-06-29T13:59:44Z The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.) Debat, Humberto Julio Grabiele, Mauro Aguilera, Patricia Mabel Bubillo, Rosana Elizabeth Zapata, Pedro Darío Marti, Dardo Andrea Ducasse, Daniel Adrian Ilex paraguariensis Virus de las Plantas Genomas Mate Plant Viruses Genomes Yerba Mate Endornavirus We present the first report of a virus infecting the subtropical tree crop yerba mate (Ilex paraguariensis St. Hil.). Total RNA purification, followed by next-generation sequencing, transcripts assembly and annotation, resulted in the identification of a new endornavirus species infecting yerba mate. The complete sequence of the linear dsRNA viral genome is 13,954-nt long, contains a single 13,743 nt ORF, and presents a 149 nt 5′UTR and a 61 nt 3′UTR. The predicted ORF encodes a 4,581 aa polypeptide with a UDP-glucose glycosyl-transferase, a capsular polysaccharide synthesis protein, and a RNA-dependent RNA polymerase domain. The name yerba mate endornavirus is proposed for the identified virus. Due to the intriguing peculiarities of this virus family, and the complete lack of the yerba mate virus literature, we consider that the information reported here will be helpful in leading to a new and needed attention to this important topic and crop. Instituto de Patología Vegetal Estación Experimental Agropecuaria Cerro Azul Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina Fil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina Fil: Bubillo, Rosana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Instituto de Biotecnología de Misiones; Argentina Fil: Marti, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2018-04-13T12:50:46Z 2018-04-13T12:50:46Z 2014-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007%2Fs11262-014-1096-2 http://hdl.handle.net/20.500.12123/2241 0920-8569 (Print) 1572-994X (Online) https://doi.org/10.1007/s11262-014-1096-2 eng info:eu-repo/semantics/restrictedAccess application/pdf Virus genes 49 (2) : 348–350. (October 2014) |
| spellingShingle | Ilex paraguariensis Virus de las Plantas Genomas Mate Plant Viruses Genomes Yerba Mate Endornavirus Debat, Humberto Julio Grabiele, Mauro Aguilera, Patricia Mabel Bubillo, Rosana Elizabeth Zapata, Pedro Darío Marti, Dardo Andrea Ducasse, Daniel Adrian The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.) |
| title | The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.) |
| title_full | The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.) |
| title_fullStr | The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.) |
| title_full_unstemmed | The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.) |
| title_short | The complete genome of a putative endornavirus identified in yerba mate (Ilex paraguariensis St. Hil.) |
| title_sort | complete genome of a putative endornavirus identified in yerba mate ilex paraguariensis st hil |
| topic | Ilex paraguariensis Virus de las Plantas Genomas Mate Plant Viruses Genomes Yerba Mate Endornavirus |
| url | https://link.springer.com/article/10.1007%2Fs11262-014-1096-2 http://hdl.handle.net/20.500.12123/2241 https://doi.org/10.1007/s11262-014-1096-2 |
| work_keys_str_mv | AT debathumbertojulio thecompletegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil AT grabielemauro thecompletegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil AT aguilerapatriciamabel thecompletegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil AT bubillorosanaelizabeth thecompletegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil AT zapatapedrodario thecompletegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil AT martidardoandrea thecompletegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil AT ducassedanieladrian thecompletegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil AT debathumbertojulio completegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil AT grabielemauro completegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil AT aguilerapatriciamabel completegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil AT bubillorosanaelizabeth completegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil AT zapatapedrodario completegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil AT martidardoandrea completegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil AT ducassedanieladrian completegenomeofaputativeendornavirusidentifiedinyerbamateilexparaguariensissthil |