Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology
In Argentina, various studies have reported the detection of multiple viruses in honey-producing and queen-rearing apiaries, with Aparavirus apisacutum, the causal agent of acute bee paralysis (ABP), demonstrating a particularly high prevalence. The potential of RNA interference (RNAi) as a strategy...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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MDPI
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/22390 https://www.mdpi.com/2075-4450/16/5/453 https://doi.org/10.3390/insects16050453 |
| _version_ | 1855486930358108160 |
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| author | Ferrufino, Cecilia Gabriela Scannapieco, Alejandra Carla Russo, Romina Maria Gonzalez, Fernanda Noemi Salvador, Ricardo Dus Santos, Maria Jose |
| author_browse | Dus Santos, Maria Jose Ferrufino, Cecilia Gabriela Gonzalez, Fernanda Noemi Russo, Romina Maria Salvador, Ricardo Scannapieco, Alejandra Carla |
| author_facet | Ferrufino, Cecilia Gabriela Scannapieco, Alejandra Carla Russo, Romina Maria Gonzalez, Fernanda Noemi Salvador, Ricardo Dus Santos, Maria Jose |
| author_sort | Ferrufino, Cecilia Gabriela |
| collection | INTA Digital |
| description | In Argentina, various studies have reported the detection of multiple viruses in honey-producing and queen-rearing apiaries, with Aparavirus apisacutum, the causal agent of acute bee paralysis (ABP), demonstrating a particularly high prevalence. The potential of RNA interference (RNAi) as a strategy to control honey bee viruses has been explored, with initial findings indicating that RNAi could aid in mitigating the economic losses associated with viral infections. This study aimed to evaluate the effect of RNAi technology mediated by double-stranded RNA (dsRNA) on the dynamics of ABPV infection in adult honey bees. Fragments of the ABPV replicase and VP1 genes were used as templates for dsRNA synthesis via in vitro transcription. A gene silencing experiment was conducted through oral administration using five treatments: control, specific dsRNA + Virus, Virus alone, specific dsRNA alone, and non-specific dsRNA + virus. Bee survival was recorded over 10 days for all treatments, and samples were subsequently processed for viral quantification using quantitative real-time PCR. The oral administration of specific dsRNA reduced the viral replication curve, decreased the average viral loads and increased bee survival. This is the first report demonstrating the reduction in ABPV infection in adult honey bees through post-transcriptional gene silencing achieved via oral administration of dsRNA. |
| format | Artículo |
| id | INTA22390 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA223902025-05-22T14:48:07Z Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology Ferrufino, Cecilia Gabriela Scannapieco, Alejandra Carla Russo, Romina Maria Gonzalez, Fernanda Noemi Salvador, Ricardo Dus Santos, Maria Jose Acute Bee Paralysis Virus dsRNA Viruses Honey Bees Survival Apis mellifera Gene Silencing Virus de Parálisis Aguda de la Abeja Virus ARN Bicatenario Abeja Melífera Supervivencia Silenciamiento Genético Argentina Aparavirus apisacutum In Argentina, various studies have reported the detection of multiple viruses in honey-producing and queen-rearing apiaries, with Aparavirus apisacutum, the causal agent of acute bee paralysis (ABP), demonstrating a particularly high prevalence. The potential of RNA interference (RNAi) as a strategy to control honey bee viruses has been explored, with initial findings indicating that RNAi could aid in mitigating the economic losses associated with viral infections. This study aimed to evaluate the effect of RNAi technology mediated by double-stranded RNA (dsRNA) on the dynamics of ABPV infection in adult honey bees. Fragments of the ABPV replicase and VP1 genes were used as templates for dsRNA synthesis via in vitro transcription. A gene silencing experiment was conducted through oral administration using five treatments: control, specific dsRNA + Virus, Virus alone, specific dsRNA alone, and non-specific dsRNA + virus. Bee survival was recorded over 10 days for all treatments, and samples were subsequently processed for viral quantification using quantitative real-time PCR. The oral administration of specific dsRNA reduced the viral replication curve, decreased the average viral loads and increased bee survival. This is the first report demonstrating the reduction in ABPV infection in adult honey bees through post-transcriptional gene silencing achieved via oral administration of dsRNA. Instituto de Virología Fil: Ferrufino, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Ferrufino, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonzalez, Fernanda Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Gonzalez, Fernanda Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Dus Santos, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-05-22T14:40:49Z 2025-05-22T14:40:49Z 2025-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22390 https://www.mdpi.com/2075-4450/16/5/453 2075-4450 https://doi.org/10.3390/insects16050453 eng info:eu-repograntAgreement/INTA/2023-PE-L01-I069, Aportes al desarrollo sostenible de la apicultura argentina info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Insects 16 (5) : 453 (May 2025) |
| spellingShingle | Acute Bee Paralysis Virus dsRNA Viruses Honey Bees Survival Apis mellifera Gene Silencing Virus de Parálisis Aguda de la Abeja Virus ARN Bicatenario Abeja Melífera Supervivencia Silenciamiento Genético Argentina Aparavirus apisacutum Ferrufino, Cecilia Gabriela Scannapieco, Alejandra Carla Russo, Romina Maria Gonzalez, Fernanda Noemi Salvador, Ricardo Dus Santos, Maria Jose Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology |
| title | Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology |
| title_full | Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology |
| title_fullStr | Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology |
| title_full_unstemmed | Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology |
| title_short | Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology |
| title_sort | reduction in acute bee paralysis virus infection and mortality in honey bees apis mellifera by rna interference technology |
| topic | Acute Bee Paralysis Virus dsRNA Viruses Honey Bees Survival Apis mellifera Gene Silencing Virus de Parálisis Aguda de la Abeja Virus ARN Bicatenario Abeja Melífera Supervivencia Silenciamiento Genético Argentina Aparavirus apisacutum |
| url | http://hdl.handle.net/20.500.12123/22390 https://www.mdpi.com/2075-4450/16/5/453 https://doi.org/10.3390/insects16050453 |
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