An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme
In this work, we described an in vitro system adequate for investigating the pathosystem soybean/arbuscular mycorrhizal fungi (AMF)/Fusarium virguliforme. Pre-mycorrhized plantlets with Rhizophagus irregularis were infected by F. virguliforme either locally via a plug of gel supporting mycelium (Met...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://www.frontiersin.org/articles/10.3389/fpls.2017.01033/full http://hdl.handle.net/20.500.12123/2238 https://doi.org/10.3389/fpls.2017.01033 |
| _version_ | 1855483019552358400 |
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| author | Giachero, María Lorena Marquez, Nathalie Gallou, Adrien Luna, Celina Mercedes Declerck, Stéphane Ducasse, Daniel Adrian |
| author_browse | Declerck, Stéphane Ducasse, Daniel Adrian Gallou, Adrien Giachero, María Lorena Luna, Celina Mercedes Marquez, Nathalie |
| author_facet | Giachero, María Lorena Marquez, Nathalie Gallou, Adrien Luna, Celina Mercedes Declerck, Stéphane Ducasse, Daniel Adrian |
| author_sort | Giachero, María Lorena |
| collection | INTA Digital |
| description | In this work, we described an in vitro system adequate for investigating the pathosystem soybean/arbuscular mycorrhizal fungi (AMF)/Fusarium virguliforme. Pre-mycorrhized plantlets with Rhizophagus irregularis were infected by F. virguliforme either locally via a plug of gel supporting mycelium (Method 1) or via a macroconidia suspension applied to the medium surface (Method 2). Root colonization by the AMF and infection by the pathogen were similar to the usual observations in pot experiments. Within a period of 18 days, more than 20% of the roots were colonized by the AMF and infection by the pathogen was observed in all the plants. In presence of AMF, a decrease in symptoms and in the level of root tissue infection was noticed. With Method 1, smaller necrotic lesions were observed in the pre-mycorrhized plantlets. In Method 2, pathogen infection was slower but more homogenous. These results demonstrated the suitability of the in vitro cultivation system to study the pathosystem soybean/AMF/F. virguliforme. We propose this in vitro cultivation system for studying the mechanisms involved in the biocontrol conferred by AMF against F. virguliforme in soybean. |
| format | Artículo |
| id | INTA2238 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA22382018-04-13T11:45:35Z An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme Giachero, María Lorena Marquez, Nathalie Gallou, Adrien Luna, Celina Mercedes Declerck, Stéphane Ducasse, Daniel Adrian Soja Rhizophagus Fusarium Patógenos Suelo Organismos Transmitidos por Suelo Soybeans Pathogens Soil Soilborne Organisms In Vitro Rhizophagus irregularis Fusarium virguliforme In this work, we described an in vitro system adequate for investigating the pathosystem soybean/arbuscular mycorrhizal fungi (AMF)/Fusarium virguliforme. Pre-mycorrhized plantlets with Rhizophagus irregularis were infected by F. virguliforme either locally via a plug of gel supporting mycelium (Method 1) or via a macroconidia suspension applied to the medium surface (Method 2). Root colonization by the AMF and infection by the pathogen were similar to the usual observations in pot experiments. Within a period of 18 days, more than 20% of the roots were colonized by the AMF and infection by the pathogen was observed in all the plants. In presence of AMF, a decrease in symptoms and in the level of root tissue infection was noticed. With Method 1, smaller necrotic lesions were observed in the pre-mycorrhized plantlets. In Method 2, pathogen infection was slower but more homogenous. These results demonstrated the suitability of the in vitro cultivation system to study the pathosystem soybean/AMF/F. virguliforme. We propose this in vitro cultivation system for studying the mechanisms involved in the biocontrol conferred by AMF against F. virguliforme in soybean. Instituto de Patología Vegetal Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Giachero, María Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gallou, Adrien. Centro Nacional de Referencia de Control Biológico; México Fil: Luna, Celina Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Declerck, Stéphane. Université Catholique de Louvain. Earth and Life Institute, Applied Microbiology, Mycology; Bélgica Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2018-04-13T11:41:53Z 2018-04-13T11:41:53Z 2017-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.frontiersin.org/articles/10.3389/fpls.2017.01033/full http://hdl.handle.net/20.500.12123/2238 1664-462X https://doi.org/10.3389/fpls.2017.01033 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers in Plant Science 8 : 1033 (June 2017) |
| spellingShingle | Soja Rhizophagus Fusarium Patógenos Suelo Organismos Transmitidos por Suelo Soybeans Pathogens Soil Soilborne Organisms In Vitro Rhizophagus irregularis Fusarium virguliforme Giachero, María Lorena Marquez, Nathalie Gallou, Adrien Luna, Celina Mercedes Declerck, Stéphane Ducasse, Daniel Adrian An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme |
| title | An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme |
| title_full | An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme |
| title_fullStr | An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme |
| title_full_unstemmed | An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme |
| title_short | An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme |
| title_sort | in vitro method for studying the three way interaction between soybean rhizophagus irregularis and the soil borne pathogen fusarium virguliforme |
| topic | Soja Rhizophagus Fusarium Patógenos Suelo Organismos Transmitidos por Suelo Soybeans Pathogens Soil Soilborne Organisms In Vitro Rhizophagus irregularis Fusarium virguliforme |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2017.01033/full http://hdl.handle.net/20.500.12123/2238 https://doi.org/10.3389/fpls.2017.01033 |
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