Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay
Vector-borne hemoparasitic infections are a major problem that affects livestock industries worldwide, particularly in tropical and subtropical regions. In this work, a reverse line blot (RLB) hybridization assay was developed for the simultaneous detection and identification of Anaplasma, Babesia...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2237 https://doi.org/10.1016/j.ttbdis.2017.08.011 |
| _version_ | 1855483019360468992 |
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| author | Paoletta, Martina Lopez Arias, Ludmila Sol De La Fourniere, Sofia Guillemi, Eliana Carolina Luciani, Carlos Sarmiento, Nestor Fabian Mosqueda Gualito, Juan Joel Farber, Marisa Diana Wilkowsky, Silvina Elizabeth |
| author_browse | De La Fourniere, Sofia Farber, Marisa Diana Guillemi, Eliana Carolina Lopez Arias, Ludmila Sol Luciani, Carlos Mosqueda Gualito, Juan Joel Paoletta, Martina Sarmiento, Nestor Fabian Wilkowsky, Silvina Elizabeth |
| author_facet | Paoletta, Martina Lopez Arias, Ludmila Sol De La Fourniere, Sofia Guillemi, Eliana Carolina Luciani, Carlos Sarmiento, Nestor Fabian Mosqueda Gualito, Juan Joel Farber, Marisa Diana Wilkowsky, Silvina Elizabeth |
| author_sort | Paoletta, Martina |
| collection | INTA Digital |
| description | Vector-borne hemoparasitic infections are a major problem that affects livestock industries worldwide, particularly in tropical and subtropical regions. In this work, a reverse line blot (RLB) hybridization assay was developed
for the simultaneous detection and identification of Anaplasma, Babesia and bovine trypanosomes, encompassing
in this way the most relevant hemoparasites that affect cattle. A total of 186 bovine blood samples
collected from two different ecoepidemiological regions of northeast Argentina, with and without tick control, were analyzed with this new RLB. High diversity of parasites, such as Babesia bovis, B. bigemina, Anaplasma marginale and three different Trypanosoma species, was found. High rates of coinfections were also detected, and significant differences were observed not only in the prevalence of parasites but also in the level of coinfections
between the two analyzed areas. Regarding the Trypanosoma genus, we provide molecular evidence of the
presence of T. vivax and T. theileri for the first time in Argentina. Besides, since the RLB is a prospective tool, it
allowed the identification of a yet unknown bovine trypanosome which could not be assigned to any of the bovine species known so far. In the present study we provide new insights on the prevalence of several pathogens that directly impact on livestock production in Argentina. The RLB assay developed here allows to identify simultaneously numerous pathogenic species which can also be easily expanded to detect other blood borne pathogens. These characteristics make the RLB hybridization assay an essential tool for epidemiological survey
of all vector-borne pathogens. |
| format | Artículo |
| id | INTA2237 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA22372021-12-07T10:50:16Z Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay Paoletta, Martina Lopez Arias, Ludmila Sol De La Fourniere, Sofia Guillemi, Eliana Carolina Luciani, Carlos Sarmiento, Nestor Fabian Mosqueda Gualito, Juan Joel Farber, Marisa Diana Wilkowsky, Silvina Elizabeth Bovina Babesia Anaplasma Trypanosoma Enfermedades Transmitidas Vectores Ganado Bovino Cattle Vectorborne Diseases Hybridization Bovinae Epidemiología Reverse Line Blot Hybridization Hibridación de Transferencia de Línea Inversa Vector-borne hemoparasitic infections are a major problem that affects livestock industries worldwide, particularly in tropical and subtropical regions. In this work, a reverse line blot (RLB) hybridization assay was developed for the simultaneous detection and identification of Anaplasma, Babesia and bovine trypanosomes, encompassing in this way the most relevant hemoparasites that affect cattle. A total of 186 bovine blood samples collected from two different ecoepidemiological regions of northeast Argentina, with and without tick control, were analyzed with this new RLB. High diversity of parasites, such as Babesia bovis, B. bigemina, Anaplasma marginale and three different Trypanosoma species, was found. High rates of coinfections were also detected, and significant differences were observed not only in the prevalence of parasites but also in the level of coinfections between the two analyzed areas. Regarding the Trypanosoma genus, we provide molecular evidence of the presence of T. vivax and T. theileri for the first time in Argentina. Besides, since the RLB is a prospective tool, it allowed the identification of a yet unknown bovine trypanosome which could not be assigned to any of the bovine species known so far. In the present study we provide new insights on the prevalence of several pathogens that directly impact on livestock production in Argentina. The RLB assay developed here allows to identify simultaneously numerous pathogenic species which can also be easily expanded to detect other blood borne pathogens. These characteristics make the RLB hybridization assay an essential tool for epidemiological survey of all vector-borne pathogens. Instituto de Biotecnología Estación Experimental Agropecuaria Colonia Benitez Estación Experimental Agropecuaria Mercedes Fil: Paoletta, Martina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Lopez Arias, Ludmila Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: De La Fourniere, Sofia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Luciani, Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina Fil: Sarmiento, Nestor Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Mosqueda Gualito, Juan Joel. Universidad Autónoma de Querétaro. Facultad de Ciencias Naturales; México Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2018-04-12T18:35:55Z 2018-04-12T18:35:55Z 2018-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2237 1877-959X https://doi.org/10.1016/j.ttbdis.2017.08.011 eng info:eu-repo/semantics/restrictedAccess application/pdf Ticks and tick-borne diseases 9 (2) : 155-163. (February 2018) |
| spellingShingle | Bovina Babesia Anaplasma Trypanosoma Enfermedades Transmitidas Vectores Ganado Bovino Cattle Vectorborne Diseases Hybridization Bovinae Epidemiología Reverse Line Blot Hybridization Hibridación de Transferencia de Línea Inversa Paoletta, Martina Lopez Arias, Ludmila Sol De La Fourniere, Sofia Guillemi, Eliana Carolina Luciani, Carlos Sarmiento, Nestor Fabian Mosqueda Gualito, Juan Joel Farber, Marisa Diana Wilkowsky, Silvina Elizabeth Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay |
| title | Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay |
| title_full | Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay |
| title_fullStr | Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay |
| title_full_unstemmed | Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay |
| title_short | Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay |
| title_sort | epidemiology of babesia anaplasma and trypanosoma species using a new expanded reverse line blot hybridization assay |
| topic | Bovina Babesia Anaplasma Trypanosoma Enfermedades Transmitidas Vectores Ganado Bovino Cattle Vectorborne Diseases Hybridization Bovinae Epidemiología Reverse Line Blot Hybridization Hibridación de Transferencia de Línea Inversa |
| url | http://hdl.handle.net/20.500.12123/2237 https://doi.org/10.1016/j.ttbdis.2017.08.011 |
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