Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina
The occurrence and genetic diversity of Cydia pomonella granulovirus infecting C. pomonella across Argentina were investigated. Larvae were collected from fruit orchards in regions where CpGV‐based biopesticides have been extensively used and then discontinued (Catamarca) or are still being used (Al...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12162 http://hdl.handle.net/20.500.12123/2233 https://doi.org/10.1111/aab.12162 |
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| author | Arneodo Larochette, Joel Demian De Anna, J. Salvador, Ricardo Farinon, Omar Marcelo Quintana, Graciela Mabel Sciocco, Alicia Ines |
| author_browse | Arneodo Larochette, Joel Demian De Anna, J. Farinon, Omar Marcelo Quintana, Graciela Mabel Salvador, Ricardo Sciocco, Alicia Ines |
| author_facet | Arneodo Larochette, Joel Demian De Anna, J. Salvador, Ricardo Farinon, Omar Marcelo Quintana, Graciela Mabel Sciocco, Alicia Ines |
| author_sort | Arneodo Larochette, Joel Demian |
| collection | INTA Digital |
| description | The occurrence and genetic diversity of Cydia pomonella granulovirus infecting C. pomonella across Argentina were investigated. Larvae were collected from fruit orchards in regions where CpGV‐based biopesticides have been extensively used and then discontinued (Catamarca) or are still being used (Alto Valle). The survey was also extended to regions where few occasional (i.e. Mendoza) or no virus treatments (various locations) have been made. PCR analysis showed an unexpectedly high proportion of infected larvae (31.3–50%) at most sampling sites in Catamarca. Phylogenetic analysis of these samples, based on gran and lef‐8 genes partial sequences, revealed a previously unreported CpGV variant which could not be assigned to any of the known CpGV genome types and formed a well‐defined new cluster. Instead, samples from Alto Valle were genetically similar to isolate CpGV‐M (the active ingredient in commercial formulations). There, the percentage of infected individuals was considerably lower (1.8–21.9%). The virus was not detected in Mendoza or the other sampling regions. Implications of these results on the epidemiology and current classification of CpGV isolates are discussed. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2233 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA22332018-06-26T18:08:09Z Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina Arneodo Larochette, Joel Demian De Anna, J. Salvador, Ricardo Farinon, Omar Marcelo Quintana, Graciela Mabel Sciocco, Alicia Ines Cydia Pomonella Lepidoptera Baculoviridae Diversidad Genética como Recurso Virus de las Plantas Genetic Diversity as Resource Plant Viruses Granulovirus Argentina The occurrence and genetic diversity of Cydia pomonella granulovirus infecting C. pomonella across Argentina were investigated. Larvae were collected from fruit orchards in regions where CpGV‐based biopesticides have been extensively used and then discontinued (Catamarca) or are still being used (Alto Valle). The survey was also extended to regions where few occasional (i.e. Mendoza) or no virus treatments (various locations) have been made. PCR analysis showed an unexpectedly high proportion of infected larvae (31.3–50%) at most sampling sites in Catamarca. Phylogenetic analysis of these samples, based on gran and lef‐8 genes partial sequences, revealed a previously unreported CpGV variant which could not be assigned to any of the known CpGV genome types and formed a well‐defined new cluster. Instead, samples from Alto Valle were genetically similar to isolate CpGV‐M (the active ingredient in commercial formulations). There, the percentage of infected individuals was considerably lower (1.8–21.9%). The virus was not detected in Mendoza or the other sampling regions. Implications of these results on the epidemiology and current classification of CpGV isolates are discussed. Instituto de Microbiología y Zoología Agrícola Fil: Arneodo Larochette, Joel Demian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: De Anna, J. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Farinon, Omar Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Quintana, Graciela Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina 2018-04-12T16:58:18Z 2018-04-12T16:58:18Z 2015-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12162 http://hdl.handle.net/20.500.12123/2233 0003-4746 1744-7348 https://doi.org/10.1111/aab.12162 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Annals of applied biology 166 (1) : 67-74. (January 2015) |
| spellingShingle | Cydia Pomonella Lepidoptera Baculoviridae Diversidad Genética como Recurso Virus de las Plantas Genetic Diversity as Resource Plant Viruses Granulovirus Argentina Arneodo Larochette, Joel Demian De Anna, J. Salvador, Ricardo Farinon, Omar Marcelo Quintana, Graciela Mabel Sciocco, Alicia Ines Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina |
| title | Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina |
| title_full | Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina |
| title_fullStr | Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina |
| title_full_unstemmed | Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina |
| title_short | Prospection and molecular analysis of CpGV isolates infecting Cydia pomonella at different geographical locations in Argentina |
| title_sort | prospection and molecular analysis of cpgv isolates infecting cydia pomonella at different geographical locations in argentina |
| topic | Cydia Pomonella Lepidoptera Baculoviridae Diversidad Genética como Recurso Virus de las Plantas Genetic Diversity as Resource Plant Viruses Granulovirus Argentina |
| url | https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12162 http://hdl.handle.net/20.500.12123/2233 https://doi.org/10.1111/aab.12162 |
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