Galectin‐8 activates dendritic cells and stimulates antigen‐specific immune response elicitation
Galectin‐8 (Gal‐8) is a mammalian β‐galactoside‐binding lectin, endowed with proinflammatory properties. Given its capacity to enhance antigen‐specific immune responses in vivo, we investigated whether Gal‐8 was also able to promote APC activation to sustain T cell activation after priming. Both end...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://jlb.onlinelibrary.wiley.com/doi/full/10.1189/jlb.3A0816-357RR http://hdl.handle.net/20.500.12123/2232 https://doi.org/10.1189/jlb.3A0816-357RR |
| _version_ | 1855483018617028608 |
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| author | Carabelli, Julieta Quattrocchi, Valeria D'Antuono, Alejandra Zamorano, Patricia Ines Tribulatti, María Virginia Campetella, Oscar |
| author_browse | Campetella, Oscar Carabelli, Julieta D'Antuono, Alejandra Quattrocchi, Valeria Tribulatti, María Virginia Zamorano, Patricia Ines |
| author_facet | Carabelli, Julieta Quattrocchi, Valeria D'Antuono, Alejandra Zamorano, Patricia Ines Tribulatti, María Virginia Campetella, Oscar |
| author_sort | Carabelli, Julieta |
| collection | INTA Digital |
| description | Galectin‐8 (Gal‐8) is a mammalian β‐galactoside‐binding lectin, endowed with proinflammatory properties. Given its capacity to enhance antigen‐specific immune responses in vivo, we investigated whether Gal‐8 was also able to promote APC activation to sustain T cell activation after priming. Both endogenous [dendritic cells (DCs)] and bone marrow‐derived DCs (BMDCs) treated with exogenous Gal‐8 exhibited a mature phenotype characterized by increased MHC class II (MHCII), CD80, and CD86 surface expression. Moreover, Gal‐8‐treated BMDCs (Gal‐8–BMDCs) stimulated antigen‐specific T cells more efficiently than immature BMDCs (iBMDCs). Proinflammatory cytokines IL‐3, IL‐2, IL‐6, TNF, MCP‐1, and MCP‐5, as well as growth factor G‐CSF, were augmented in Gal‐8–BMDC conditioned media, with IL‐6 as the most prominent. Remarkably, BMDCs from Gal‐8‐deficient mice (Lgals8−/− BMDC) displayed reduced CD86 and IL‐6 expression and an impaired ability to promote antigen‐specific CD4 T cell activation. To test if Gal‐8‐induced activation correlates with the elicitation of an effective immune response, soluble Gal‐8 was coadministrated with antigen during immunization of BALB/cJ mice in the experimental foot‐and‐mouth disease virus (FMDV) model. When a single dose of Gal‐8 was added to the antigen formulation, an increased specific and neutralizing humoral response was developed, sufficient to enhance animal protection upon viral challenge. IL‐6 and IFN‐γ, as well as lymphoproliferative responses, were also incremented in Gal‐8/antigen‐immunized animals only at 48 h after immunization, suggesting that Gal‐8 induces the elicitation of an inflammatory response at an early stage. Taking together, these findings argue in favor of the use of Gal‐8 as an immune‐stimulator molecule to enhance the adaptive immune response. |
| format | Artículo |
| id | INTA2232 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA22322018-06-21T12:45:58Z Galectin‐8 activates dendritic cells and stimulates antigen‐specific immune response elicitation Carabelli, Julieta Quattrocchi, Valeria D'Antuono, Alejandra Zamorano, Patricia Ines Tribulatti, María Virginia Campetella, Oscar Lectinas Células Inmunización Respuesta Inmunológica Ratón Lectins Cells Immunization Immune Response Mice Galectin-8 Galectin‐8 (Gal‐8) is a mammalian β‐galactoside‐binding lectin, endowed with proinflammatory properties. Given its capacity to enhance antigen‐specific immune responses in vivo, we investigated whether Gal‐8 was also able to promote APC activation to sustain T cell activation after priming. Both endogenous [dendritic cells (DCs)] and bone marrow‐derived DCs (BMDCs) treated with exogenous Gal‐8 exhibited a mature phenotype characterized by increased MHC class II (MHCII), CD80, and CD86 surface expression. Moreover, Gal‐8‐treated BMDCs (Gal‐8–BMDCs) stimulated antigen‐specific T cells more efficiently than immature BMDCs (iBMDCs). Proinflammatory cytokines IL‐3, IL‐2, IL‐6, TNF, MCP‐1, and MCP‐5, as well as growth factor G‐CSF, were augmented in Gal‐8–BMDC conditioned media, with IL‐6 as the most prominent. Remarkably, BMDCs from Gal‐8‐deficient mice (Lgals8−/− BMDC) displayed reduced CD86 and IL‐6 expression and an impaired ability to promote antigen‐specific CD4 T cell activation. To test if Gal‐8‐induced activation correlates with the elicitation of an effective immune response, soluble Gal‐8 was coadministrated with antigen during immunization of BALB/cJ mice in the experimental foot‐and‐mouth disease virus (FMDV) model. When a single dose of Gal‐8 was added to the antigen formulation, an increased specific and neutralizing humoral response was developed, sufficient to enhance animal protection upon viral challenge. IL‐6 and IFN‐γ, as well as lymphoproliferative responses, were also incremented in Gal‐8/antigen‐immunized animals only at 48 h after immunization, suggesting that Gal‐8 induces the elicitation of an inflammatory response at an early stage. Taking together, these findings argue in favor of the use of Gal‐8 as an immune‐stimulator molecule to enhance the adaptive immune response. Instituto de Virología Fil: Carabelli, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Laboratorio de Inmunología Molecular; Argentina Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: D'Antuono, Alejandra. Instituto de Ciencias y Tecnología “Dr. Cesar Milstein”. Centro de Virología Animal; Argentina Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Tribulatti, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Laboratorio de Inmunología Molecular; Argentina Fil: Campetella, Oscar Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Laboratorio de Inmunología Molecular; Argentina 2018-04-12T15:33:34Z 2018-04-12T15:33:34Z 2017-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://jlb.onlinelibrary.wiley.com/doi/full/10.1189/jlb.3A0816-357RR http://hdl.handle.net/20.500.12123/2232 0741-5400 1938-3673 https://doi.org/10.1189/jlb.3A0816-357RR eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of leukocyte biology 102 (5) : 1237-1247. (November 2017) |
| spellingShingle | Lectinas Células Inmunización Respuesta Inmunológica Ratón Lectins Cells Immunization Immune Response Mice Galectin-8 Carabelli, Julieta Quattrocchi, Valeria D'Antuono, Alejandra Zamorano, Patricia Ines Tribulatti, María Virginia Campetella, Oscar Galectin‐8 activates dendritic cells and stimulates antigen‐specific immune response elicitation |
| title | Galectin‐8 activates dendritic cells and stimulates antigen‐specific immune response elicitation |
| title_full | Galectin‐8 activates dendritic cells and stimulates antigen‐specific immune response elicitation |
| title_fullStr | Galectin‐8 activates dendritic cells and stimulates antigen‐specific immune response elicitation |
| title_full_unstemmed | Galectin‐8 activates dendritic cells and stimulates antigen‐specific immune response elicitation |
| title_short | Galectin‐8 activates dendritic cells and stimulates antigen‐specific immune response elicitation |
| title_sort | galectin 8 activates dendritic cells and stimulates antigen specific immune response elicitation |
| topic | Lectinas Células Inmunización Respuesta Inmunológica Ratón Lectins Cells Immunization Immune Response Mice Galectin-8 |
| url | https://jlb.onlinelibrary.wiley.com/doi/full/10.1189/jlb.3A0816-357RR http://hdl.handle.net/20.500.12123/2232 https://doi.org/10.1189/jlb.3A0816-357RR |
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