Prevalence and viability of group A rotavirus in dairy farm water sources
Aim: To analyse group A rotavirus (RVA) environmental contamination in waters used for calves' consumption and to assess viral viability in dairy farm water sources. Methods and Results: We analysed 202 samples of water used for calves' consumption and RVA was detected by RT‐qPCR in 35·1% (95% CI:...
| Main Authors: | , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13691 http://hdl.handle.net/20.500.12123/2230 https://doi.org/10.1111/jam.13691 |
| _version_ | 1855034926791917568 |
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| author | Castells, M.L. Schild, Carlos Omar Caffarena, Rubén Dario Bok, Marina Giannitti, Federico Armendano, Joaquín Ignacio Riet‐Correa, Franklin Victoria, M. Parreño, Viviana Colina, Rodney |
| author_browse | Armendano, Joaquín Ignacio Bok, Marina Caffarena, Rubén Dario Castells, M.L. Colina, Rodney Giannitti, Federico Parreño, Viviana Riet‐Correa, Franklin Schild, Carlos Omar Victoria, M. |
| author_facet | Castells, M.L. Schild, Carlos Omar Caffarena, Rubén Dario Bok, Marina Giannitti, Federico Armendano, Joaquín Ignacio Riet‐Correa, Franklin Victoria, M. Parreño, Viviana Colina, Rodney |
| author_sort | Castells, M.L. |
| collection | INTA Digital |
| description | Aim: To analyse group A rotavirus (RVA) environmental contamination in waters used for calves' consumption and to assess viral viability in dairy farm water sources. Methods and Results: We analysed 202 samples of water used for calves' consumption and RVA was detected by RT‐qPCR in 35·1% (95% CI: 28·9–42·0%). A marked pattern of seasonality was observed with higher frequency of detection in colder than warmer months (P = 0·002). There was no association between viral load and season or between the number of milking cows in the herd and the detection of RVA in the farm. The viability of the RVA particles detected was confirmed by isolation of RVA in cell culture from 5 of 10 water samples. Furthermore, an RVA waterborne outbreak of neonatal calf diarrhoea was described. Conclusions: We demonstrate that RVA is frequent in dairy farm waters, and that the virus is infectious and capable of generating a diarrhoea outbreak. Significance and Impact of the Study: Neonatal diarrhoea syndrome leads to economic losses to the livestock industry worldwide. To determine transmission routes is essential to take action in this regard and reduce the impact that this syndrome has for the livestock production. The results obtained in this work alert the dairy industry and highlight that mitigation strategies are crucial to improve the microbiological quality of this water. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2230 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA22302019-06-07T17:09:55Z Prevalence and viability of group A rotavirus in dairy farm water sources Castells, M.L. Schild, Carlos Omar Caffarena, Rubén Dario Bok, Marina Giannitti, Federico Armendano, Joaquín Ignacio Riet‐Correa, Franklin Victoria, M. Parreño, Viviana Colina, Rodney Rotavirus Granjas Lecheras Enfermedades de los Animales Morbosidad Agua Dairy Farms Animal Diseases Morbidity Water Rotavirus A Prevalencia de una enfermedad Fuentes de Agua Aim: To analyse group A rotavirus (RVA) environmental contamination in waters used for calves' consumption and to assess viral viability in dairy farm water sources. Methods and Results: We analysed 202 samples of water used for calves' consumption and RVA was detected by RT‐qPCR in 35·1% (95% CI: 28·9–42·0%). A marked pattern of seasonality was observed with higher frequency of detection in colder than warmer months (P = 0·002). There was no association between viral load and season or between the number of milking cows in the herd and the detection of RVA in the farm. The viability of the RVA particles detected was confirmed by isolation of RVA in cell culture from 5 of 10 water samples. Furthermore, an RVA waterborne outbreak of neonatal calf diarrhoea was described. Conclusions: We demonstrate that RVA is frequent in dairy farm waters, and that the virus is infectious and capable of generating a diarrhoea outbreak. Significance and Impact of the Study: Neonatal diarrhoea syndrome leads to economic losses to the livestock industry worldwide. To determine transmission routes is essential to take action in this regard and reduce the impact that this syndrome has for the livestock production. The results obtained in this work alert the dairy industry and highlight that mitigation strategies are crucial to improve the microbiological quality of this water. Instituto de Virología Fil: Castells, M.L. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; Uruguay Fil: Schild, Carlos Omar. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay Fil: Caffarena, Rubén Dario. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Giannitti, Federico. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay. University of Minnesota. College of Veterinary Medicine. Veterinary Population Medicine Department; Estados Unidos Fil: Armendano, Joaquín Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Riet‐Correa, Franklin. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay Fil: Victoria, M. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; Uruguay Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Colina, Rodney. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; Uruguay 2018-04-12T14:49:09Z 2018-04-12T14:49:09Z 2018-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13691 http://hdl.handle.net/20.500.12123/2230 1364-5072 1365-2672 https://doi.org/10.1111/jam.13691 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of applied microbiology 124 (3) : 922-929. (March 2018) |
| spellingShingle | Rotavirus Granjas Lecheras Enfermedades de los Animales Morbosidad Agua Dairy Farms Animal Diseases Morbidity Water Rotavirus A Prevalencia de una enfermedad Fuentes de Agua Castells, M.L. Schild, Carlos Omar Caffarena, Rubén Dario Bok, Marina Giannitti, Federico Armendano, Joaquín Ignacio Riet‐Correa, Franklin Victoria, M. Parreño, Viviana Colina, Rodney Prevalence and viability of group A rotavirus in dairy farm water sources |
| title | Prevalence and viability of group A rotavirus in dairy farm water sources |
| title_full | Prevalence and viability of group A rotavirus in dairy farm water sources |
| title_fullStr | Prevalence and viability of group A rotavirus in dairy farm water sources |
| title_full_unstemmed | Prevalence and viability of group A rotavirus in dairy farm water sources |
| title_short | Prevalence and viability of group A rotavirus in dairy farm water sources |
| title_sort | prevalence and viability of group a rotavirus in dairy farm water sources |
| topic | Rotavirus Granjas Lecheras Enfermedades de los Animales Morbosidad Agua Dairy Farms Animal Diseases Morbidity Water Rotavirus A Prevalencia de una enfermedad Fuentes de Agua |
| url | https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13691 http://hdl.handle.net/20.500.12123/2230 https://doi.org/10.1111/jam.13691 |
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