Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h
The objective of the present work was to evaluate the efficacy of maintaining llama embryos metabolically inhibited but viable at 5 °C during 24 h. Adult females (n = 20) were used as embryo donors and were superstimulated with eCG. A total 61 embryos were non-surgically collected from 16 of the 20...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S092144881530078X http://hdl.handle.net/20.500.12123/2228 https://doi.org/10.1016/j.smallrumres.2015.10.007 |
| _version_ | 1855034926039040000 |
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| author | Aller Atucha, Juan Florencio Abalos, Marcos Acuña, Francisco Antonio Virgili, Rosana Requena, Francisco Cancino, Andrea Karina |
| author_browse | Abalos, Marcos Acuña, Francisco Antonio Aller Atucha, Juan Florencio Cancino, Andrea Karina Requena, Francisco Virgili, Rosana |
| author_facet | Aller Atucha, Juan Florencio Abalos, Marcos Acuña, Francisco Antonio Virgili, Rosana Requena, Francisco Cancino, Andrea Karina |
| author_sort | Aller Atucha, Juan Florencio |
| collection | INTA Digital |
| description | The objective of the present work was to evaluate the efficacy of maintaining llama embryos metabolically inhibited but viable at 5 °C during 24 h. Adult females (n = 20) were used as embryo donors and were superstimulated with eCG. A total 61 embryos were non-surgically collected from 16 of the 20 llamas flushed at Day 7 after mating. Fourteen excellent hatched blastocysts were stored in sterile test tubes containing 1.5 ml of TCM 199-Hepes + 10% cow serum (v/v concentration) + antibiotics and cooled to 5 °C (cooling rate = 0.5 °C/min) and maintained at 5 °C during 24 h. After this, the embryos were warmed on warming plate (38 °C), washed three times and loaded individually into 0.25 ml French straws. Fourteen adult llamas were used as recipients and embryos were transferred only into the left uterine horn of the recipient regardless of side ovulation. All recipient llamas were pregnancy tested by transrectal ultrasonography at 45 days after transfer. Three (21.5%) pregnant females were detected and delivered three normal healthy male calves at term. To our knowledge, these offspring are the first llamas produced following transfer of refrigerated embryos. In summary, llama embryos can be cooled to 5 °C and maintained in storage for up to 24 h without significant losses of viability. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2228 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA22282018-06-26T13:20:47Z Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h Aller Atucha, Juan Florencio Abalos, Marcos Acuña, Francisco Antonio Virgili, Rosana Requena, Francisco Cancino, Andrea Karina Llama Transferencia de Embriones Parto Llamas Embryo Transfer Parturition Nacimiento The objective of the present work was to evaluate the efficacy of maintaining llama embryos metabolically inhibited but viable at 5 °C during 24 h. Adult females (n = 20) were used as embryo donors and were superstimulated with eCG. A total 61 embryos were non-surgically collected from 16 of the 20 llamas flushed at Day 7 after mating. Fourteen excellent hatched blastocysts were stored in sterile test tubes containing 1.5 ml of TCM 199-Hepes + 10% cow serum (v/v concentration) + antibiotics and cooled to 5 °C (cooling rate = 0.5 °C/min) and maintained at 5 °C during 24 h. After this, the embryos were warmed on warming plate (38 °C), washed three times and loaded individually into 0.25 ml French straws. Fourteen adult llamas were used as recipients and embryos were transferred only into the left uterine horn of the recipient regardless of side ovulation. All recipient llamas were pregnancy tested by transrectal ultrasonography at 45 days after transfer. Three (21.5%) pregnant females were detected and delivered three normal healthy male calves at term. To our knowledge, these offspring are the first llamas produced following transfer of refrigerated embryos. In summary, llama embryos can be cooled to 5 °C and maintained in storage for up to 24 h without significant losses of viability. EEA Balcarce Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Abalos, Marcos. INTA, Estación Experimental Agropecuaria Abra Pampa; Argentina Fil: Acuña, Francisco Antonio. INTA, Estación Experimental Agropecuaria Abra Pampa; Argentina Fil: Virgili, Rosana. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias; Argentina Fil: Requena, Francisco. Universidad de Córdoba. Facultad de Veterinaria. Campus Universitario Rabanales; España Fil: Cancino, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina 2018-04-12T14:07:26Z 2018-04-12T14:07:26Z 2015-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S092144881530078X http://hdl.handle.net/20.500.12123/2228 0921-4488 https://doi.org/10.1016/j.smallrumres.2015.10.007 eng info:eu-repo/semantics/restrictedAccess application/pdf Small ruminant research 132 : 99-102. (November 2015) |
| spellingShingle | Llama Transferencia de Embriones Parto Llamas Embryo Transfer Parturition Nacimiento Aller Atucha, Juan Florencio Abalos, Marcos Acuña, Francisco Antonio Virgili, Rosana Requena, Francisco Cancino, Andrea Karina Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h |
| title | Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h |
| title_full | Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h |
| title_fullStr | Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h |
| title_full_unstemmed | Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h |
| title_short | Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h |
| title_sort | birth of live llama lama glama derived from embryo transfer storage at 5 °c for 24 h |
| topic | Llama Transferencia de Embriones Parto Llamas Embryo Transfer Parturition Nacimiento |
| url | https://www.sciencedirect.com/science/article/pii/S092144881530078X http://hdl.handle.net/20.500.12123/2228 https://doi.org/10.1016/j.smallrumres.2015.10.007 |
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