Use of the Micro-Agar Larval Development Test to Differentiate Resistant and Susceptible Cooperia spp. Isolates in Cattle Within the Context of Parasite Population Replacement
Gastrointestinal nematode infections are a global concern in grazing cattle production systems, even more so due to the widespread problem of anthelmintic resistance. In response, early anthelmintic resistance detection methods, such as the micro-agar larval development test (MALDT), and parasite ma...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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MDPI
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/22247 https://www.mdpi.com/2076-0817/13/11/952 https://doi.org/10.3390/pathogens13110952 |
| _version_ | 1855038539852414976 |
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| author | Fuentes, Mariana Elisabet Lloberas, María Mercedes Bernat, Gisele Anahí Riva, Eliana Junco, Milagros Fernández, Silvina |
| author_browse | Bernat, Gisele Anahí Fernández, Silvina Fuentes, Mariana Elisabet Junco, Milagros Lloberas, María Mercedes Riva, Eliana |
| author_facet | Fuentes, Mariana Elisabet Lloberas, María Mercedes Bernat, Gisele Anahí Riva, Eliana Junco, Milagros Fernández, Silvina |
| author_sort | Fuentes, Mariana Elisabet |
| collection | INTA Digital |
| description | Gastrointestinal nematode infections are a global concern in grazing cattle production systems, even more so due to the widespread problem of anthelmintic resistance. In response, early anthelmintic resistance detection methods, such as the micro-agar larval development test (MALDT), and parasite management strategies, such as the replacement of resistant parasite populations with susceptible ones, have been developed. This study aimed to characterize ivermectin-susceptible and -resistant isolates of Cooperia spp. using MALDT in the context of a parasite population replacement strategy. Three Cooperia spp. field isolates were evaluated: a susceptible one (Coop-S), a resistant one (Coop-R), and a post-replacement one (Coop-PR). The MALDT was performed in 96-well plates with 12 known concentrations of eprinomectin (EPR) on an agar base. Each test was performed in quadruplicate. Data analysis included nonlinear regression to determine EC50, EC90, and EC99 values, resistance ratios (RRs), and R2. The results showed clear differentiation between the isolates, with RR values of 5.78 and 1.28 for Coop-R and Coop-PR, respectively, compared to Coop-S. The MALDT proved to be a reliable tool for differentiating ivermectin-susceptible from ivermectin-resistant isolates of Cooperia spp., and future evaluations of this test in mixed nematode populations are recommended for routine diagnosis of anthelmintic resistance |
| format | info:ar-repo/semantics/artículo |
| id | INTA22247 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA222472025-05-13T11:46:03Z Use of the Micro-Agar Larval Development Test to Differentiate Resistant and Susceptible Cooperia spp. Isolates in Cattle Within the Context of Parasite Population Replacement Fuentes, Mariana Elisabet Lloberas, María Mercedes Bernat, Gisele Anahí Riva, Eliana Junco, Milagros Fernández, Silvina Ganado Bovino Resistencia a los Antihelmínticos Nematodos Experimentación in Vitro Cattle Cooperia Resistance to Anthelmintics Nematodes In Vitro Experimentation Nematodos Gastrointestinales Gastrointestinal Nematodes Gastrointestinal nematode infections are a global concern in grazing cattle production systems, even more so due to the widespread problem of anthelmintic resistance. In response, early anthelmintic resistance detection methods, such as the micro-agar larval development test (MALDT), and parasite management strategies, such as the replacement of resistant parasite populations with susceptible ones, have been developed. This study aimed to characterize ivermectin-susceptible and -resistant isolates of Cooperia spp. using MALDT in the context of a parasite population replacement strategy. Three Cooperia spp. field isolates were evaluated: a susceptible one (Coop-S), a resistant one (Coop-R), and a post-replacement one (Coop-PR). The MALDT was performed in 96-well plates with 12 known concentrations of eprinomectin (EPR) on an agar base. Each test was performed in quadruplicate. Data analysis included nonlinear regression to determine EC50, EC90, and EC99 values, resistance ratios (RRs), and R2. The results showed clear differentiation between the isolates, with RR values of 5.78 and 1.28 for Coop-R and Coop-PR, respectively, compared to Coop-S. The MALDT proved to be a reliable tool for differentiating ivermectin-susceptible from ivermectin-resistant isolates of Cooperia spp., and future evaluations of this test in mixed nematode populations are recommended for routine diagnosis of anthelmintic resistance EEA Balcarce Fil: Fuentes, Mariana Elisabet. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina Fil: Fuentes, Mariana Elisabet. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Fuentes, Mariana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Fuentes, Mariana Elisabet. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Lloberas, Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Lloberas, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Bernat, Gisele Anahí. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Bernat, Gisele Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Bernat, Gisele Anahí. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Riva, Eliana. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina Fil: Riva, Eliana. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Riva, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Riva, Eliana. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Junco, Milagros. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Junco, Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Junco, Milagros. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Junco, Milagros. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Sanidad Animal, Pública y Ambiental; Argentina Fil: Fernández, Silvina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Fernández, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Fernández, Silvina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Fernández, Silvina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Sanidad Animal, Pública y Ambiental; Argentina 2025-05-13T11:42:50Z 2025-05-13T11:42:50Z 2024-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22247 https://www.mdpi.com/2076-0817/13/11/952 2076-0817 https://doi.org/10.3390/pathogens13110952 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Pathogens 13 (11) : 952. (October 2024) |
| spellingShingle | Ganado Bovino Resistencia a los Antihelmínticos Nematodos Experimentación in Vitro Cattle Cooperia Resistance to Anthelmintics Nematodes In Vitro Experimentation Nematodos Gastrointestinales Gastrointestinal Nematodes Fuentes, Mariana Elisabet Lloberas, María Mercedes Bernat, Gisele Anahí Riva, Eliana Junco, Milagros Fernández, Silvina Use of the Micro-Agar Larval Development Test to Differentiate Resistant and Susceptible Cooperia spp. Isolates in Cattle Within the Context of Parasite Population Replacement |
| title | Use of the Micro-Agar Larval Development Test to Differentiate Resistant and Susceptible Cooperia spp. Isolates in Cattle Within the Context of Parasite Population Replacement |
| title_full | Use of the Micro-Agar Larval Development Test to Differentiate Resistant and Susceptible Cooperia spp. Isolates in Cattle Within the Context of Parasite Population Replacement |
| title_fullStr | Use of the Micro-Agar Larval Development Test to Differentiate Resistant and Susceptible Cooperia spp. Isolates in Cattle Within the Context of Parasite Population Replacement |
| title_full_unstemmed | Use of the Micro-Agar Larval Development Test to Differentiate Resistant and Susceptible Cooperia spp. Isolates in Cattle Within the Context of Parasite Population Replacement |
| title_short | Use of the Micro-Agar Larval Development Test to Differentiate Resistant and Susceptible Cooperia spp. Isolates in Cattle Within the Context of Parasite Population Replacement |
| title_sort | use of the micro agar larval development test to differentiate resistant and susceptible cooperia spp isolates in cattle within the context of parasite population replacement |
| topic | Ganado Bovino Resistencia a los Antihelmínticos Nematodos Experimentación in Vitro Cattle Cooperia Resistance to Anthelmintics Nematodes In Vitro Experimentation Nematodos Gastrointestinales Gastrointestinal Nematodes |
| url | http://hdl.handle.net/20.500.12123/22247 https://www.mdpi.com/2076-0817/13/11/952 https://doi.org/10.3390/pathogens13110952 |
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