In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles
Supplement: Proceedings of the 17th International Congress of the Spanish Society for Animal Reproduction (AERA), 17 to 19 of October 2024, Madrid, Spain
| Main Authors: | , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Wiley
2025
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/22225 https://onlinelibrary.wiley.com/doi/10.1111/rda.14654 https://doi.org/10.1111/rda.14654 |
| _version_ | 1855038536903819264 |
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| author | Garrappa, Gabriela Martínez López, Cristina Jiménez Movilla, María García Vázquez, Francisco Alberto |
| author_browse | García Vázquez, Francisco Alberto Garrappa, Gabriela Jiménez Movilla, María Martínez López, Cristina |
| author_facet | Garrappa, Gabriela Martínez López, Cristina Jiménez Movilla, María García Vázquez, Francisco Alberto |
| author_sort | Garrappa, Gabriela |
| collection | INTA Digital |
| description | Supplement: Proceedings of the 17th International Congress of the Spanish Society for Animal Reproduction (AERA), 17 to 19 of October 2024, Madrid, Spain |
| format | info:ar-repo/semantics/artículo |
| id | INTA22225 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA222252025-09-26T11:24:27Z In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles Garrappa, Gabriela Martínez López, Cristina Jiménez Movilla, María García Vázquez, Francisco Alberto Tecnología Cerdo Espermatozoo Nanobiotecnología Technology Swine Spermatozoa Nanobiotechnology Porcinos Supplement: Proceedings of the 17th International Congress of the Spanish Society for Animal Reproduction (AERA), 17 to 19 of October 2024, Madrid, Spain Nanotechnology and its applications have advanced significantly in recent decades, contributing to various fields, including reproduction. This study introduces a novel method to label porcine oocytes with nanoparticles (NPs) bound to oviductin (OVGP1, Ov) for use in Assisted Reproductive Technologies (ARTs). Despite promising developments, concerns about NP toxicity in gametes necessitate thorough investigation. This research aims to assess the impact of functionalized NPs (NPOv) on sperm functionality. Boar sperm were co-incubated with NPOv for 0, 0.5 and 1 h in two media: BTS (semen dilution and conservation) and TALP (sperm capacitation and in vitro fertilization-IVF). Sperm quality parameters (viability, motility and kinematics) showed no significant differences in TALP medium (p > .05). In BTS, although some kinetic parameters were altered, motility, progressive motility and viability remained unaffected (p > .05). Additionally, NPs presence on the zona pellucida (ZP) of oocytes did not affect sperm attachment (p > .05). In conclusion, in vitro exposure of boar sperm to OVGP1-functionalized NPs in IVF medium or attached to the ZP surface of matured oocytes does not impair sperm functionality, including their binding ability to the ZP. EEA Rafaela Fil: Garrappa, Gabriela. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España Fil: Garrappa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Garrappa, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Martínez López, Cristina. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España Fil: Jimenez Movilla, Maria. Instituto Murciano de Investigación Biosanitaria (IMIB); España Fil: Jimenez Movilla, Maria. Universidad de Murcia. Facultad de Medicina y Enfermería. Campus de Excelencia Mare Nostrum. Departamento de Biología Celular e Histología; España Fil: García Vázquez, Francisco Alberto. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España Fil: García Vázquez, Francisco Alberto. Instituto Murciano de Investigación Biosanitaria (IMIB); España 2025-05-09T16:20:20Z 2025-05-09T16:20:20Z 2024-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22225 https://onlinelibrary.wiley.com/doi/10.1111/rda.14654 0936-6768 1439-0531 https://doi.org/10.1111/rda.14654 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Reproduction in Domestic Animals 59 (S3) : e14654. (October 2024) |
| spellingShingle | Tecnología Cerdo Espermatozoo Nanobiotecnología Technology Swine Spermatozoa Nanobiotechnology Porcinos Garrappa, Gabriela Martínez López, Cristina Jiménez Movilla, María García Vázquez, Francisco Alberto In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles |
| title | In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles |
| title_full | In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles |
| title_fullStr | In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles |
| title_full_unstemmed | In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles |
| title_short | In vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles |
| title_sort | in vitro exposure of porcine sperm to functionalized superparamagnetic nanoparticles |
| topic | Tecnología Cerdo Espermatozoo Nanobiotecnología Technology Swine Spermatozoa Nanobiotechnology Porcinos |
| url | http://hdl.handle.net/20.500.12123/22225 https://onlinelibrary.wiley.com/doi/10.1111/rda.14654 https://doi.org/10.1111/rda.14654 |
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