Detoxification response in honey bee larvae exposed to agricultural intensification

Honey bee Apis mellifera colonies located in agroecosystems are exposed to pesticides and more fragmented habitats. The resources that bees obtain in these environments may be exposed to agrochemicals, which can accumulate in their colonies and be distributed among their nest mates. Hives placed in...

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Main Authors: Macri, Ivana Noelia, Latorre Estivalis, Jose M., Nery, Denise, Derguy, María Rosa, Cristos, Diego Sebastian, Zavala, Jorge Alberto, Farina, Walter Marcelo
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Elsevier 2025
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/22181
https://www.sciencedirect.com/science/article/abs/pii/S0048969725010253
https://doi.org/10.1016/j.scitotenv.2025.179388
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author Macri, Ivana Noelia
Latorre Estivalis, Jose M.
Nery, Denise
Derguy, María Rosa
Cristos, Diego Sebastian
Zavala, Jorge Alberto
Farina, Walter Marcelo
author_browse Cristos, Diego Sebastian
Derguy, María Rosa
Farina, Walter Marcelo
Latorre Estivalis, Jose M.
Macri, Ivana Noelia
Nery, Denise
Zavala, Jorge Alberto
author_facet Macri, Ivana Noelia
Latorre Estivalis, Jose M.
Nery, Denise
Derguy, María Rosa
Cristos, Diego Sebastian
Zavala, Jorge Alberto
Farina, Walter Marcelo
author_sort Macri, Ivana Noelia
collection INTA Digital
description Honey bee Apis mellifera colonies located in agroecosystems are exposed to pesticides and more fragmented habitats. The resources that bees obtain in these environments may be exposed to agrochemicals, which can accumulate in their colonies and be distributed among their nest mates. Hives placed in an agricultural setting located in the region of the Argentine Pampas were studied. Changes in the expression levels of insect cytochrome P450s, enzymes involved in the detoxification of xenobiotics, and the presence of pesticides in hive products at different times of crop management were evaluated. Our results showed that CYP6AS2 and CYP6AS4 expression in honey bee larvae increased significantly after crop flowering and pesticide application. Furthermore, residues of the herbicides atrazine and glyphosate, and the insecticide chlorantraniliprole were found in beeswax and honey samples collected from the same beehives, and their concentrations correlated with the expression profiles of CYP6AS2, CYP6AS3 and CYP9BD1. These results underscore the potential risks of pesticides exposure to larval development, highlighting the need to mitigate agrochemical use in agricultural landscapes to safeguard honey bee colonies.
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spelling INTA221812025-05-07T13:10:26Z Detoxification response in honey bee larvae exposed to agricultural intensification Macri, Ivana Noelia Latorre Estivalis, Jose M. Nery, Denise Derguy, María Rosa Cristos, Diego Sebastian Zavala, Jorge Alberto Farina, Walter Marcelo Abeja Melífera Agricultura Intensificación Desintoxicación Honey Bees Apis mellifera Agriculture Intensification Detoxification Agricultural Intensification Honey bee Apis mellifera colonies located in agroecosystems are exposed to pesticides and more fragmented habitats. The resources that bees obtain in these environments may be exposed to agrochemicals, which can accumulate in their colonies and be distributed among their nest mates. Hives placed in an agricultural setting located in the region of the Argentine Pampas were studied. Changes in the expression levels of insect cytochrome P450s, enzymes involved in the detoxification of xenobiotics, and the presence of pesticides in hive products at different times of crop management were evaluated. Our results showed that CYP6AS2 and CYP6AS4 expression in honey bee larvae increased significantly after crop flowering and pesticide application. Furthermore, residues of the herbicides atrazine and glyphosate, and the insecticide chlorantraniliprole were found in beeswax and honey samples collected from the same beehives, and their concentrations correlated with the expression profiles of CYP6AS2, CYP6AS3 and CYP9BD1. These results underscore the potential risks of pesticides exposure to larval development, highlighting the need to mitigate agrochemical use in agricultural landscapes to safeguard honey bee colonies. Instituto de Ingeniería Rural Fil: Macri, Ivana Noelia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Insectos Sociales; Argentina Fil: Macri, Ivana Noelia. Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE); Argentina Fil: Macri, Ivana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE); Argentina Fil: Macri, Ivana Noelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Ingeniería Rural; Argentina. Fil: Latorre Estivalis, José M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Insectos Sociales; Argentina Fil: Latorre Estivalis, José M. Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE); Argentina. Fil: Latorre Estivalis, José M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE); Argentina. Fil: Nery, Denise. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Insectos Sociales; Argentina Fil: Nery, Denise. Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE); Argentina Fil: Nery, Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE); Argentina Fil: Derguy, María Rosa. Universidad Nacional de La Plata. Laboratorio de Investigaciones de Sistemas Ecológicos y Ambientales (LISEA); Argentina Fil: Cristos, Diego Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina. Fil: Zavala, Jorge A. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Bioquímica and Cátedra de Zoología Agrícola; Argentina Fil: Zavala, Jorge A. Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA); Argentina. Fil: Zavala, Jorge A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA); Argentina Fil: Farina, Walter M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Insectos Sociales; Argentina Fil: Farina, Walter M. Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE); Argentina Fil: Farina, Walter M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE); Argentina 2025-05-07T13:07:31Z 2025-05-07T13:07:31Z 2025-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22181 https://www.sciencedirect.com/science/article/abs/pii/S0048969725010253 0048-9697 1879-1026 https://doi.org/10.1016/j.scitotenv.2025.179388 eng info:eu-repograntAgreement/INTA/2019-PE-E1-I017-001, Desarrollo del sector apícola organizado, sustentable y competitivo info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Science of The Total Environment 978 : 179388. (May 2025)
spellingShingle Abeja Melífera
Agricultura
Intensificación
Desintoxicación
Honey Bees
Apis mellifera
Agriculture
Intensification
Detoxification
Agricultural Intensification
Macri, Ivana Noelia
Latorre Estivalis, Jose M.
Nery, Denise
Derguy, María Rosa
Cristos, Diego Sebastian
Zavala, Jorge Alberto
Farina, Walter Marcelo
Detoxification response in honey bee larvae exposed to agricultural intensification
title Detoxification response in honey bee larvae exposed to agricultural intensification
title_full Detoxification response in honey bee larvae exposed to agricultural intensification
title_fullStr Detoxification response in honey bee larvae exposed to agricultural intensification
title_full_unstemmed Detoxification response in honey bee larvae exposed to agricultural intensification
title_short Detoxification response in honey bee larvae exposed to agricultural intensification
title_sort detoxification response in honey bee larvae exposed to agricultural intensification
topic Abeja Melífera
Agricultura
Intensificación
Desintoxicación
Honey Bees
Apis mellifera
Agriculture
Intensification
Detoxification
Agricultural Intensification
url http://hdl.handle.net/20.500.12123/22181
https://www.sciencedirect.com/science/article/abs/pii/S0048969725010253
https://doi.org/10.1016/j.scitotenv.2025.179388
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