Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seve...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/2214 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733342/ |
| _version_ | 1855034923637800960 |
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| author | Craig, María Isabel Rojas, María Florencia Van Der Ploeg, Claudia Andrea Olivera, Valeria Soledad Vagnozzi, Ariel Eduardo Perez, Andrés M. Konig, Guido Alberto |
| author_browse | Craig, María Isabel Konig, Guido Alberto Olivera, Valeria Soledad Perez, Andrés M. Rojas, María Florencia Vagnozzi, Ariel Eduardo Van Der Ploeg, Claudia Andrea |
| author_facet | Craig, María Isabel Rojas, María Florencia Van Der Ploeg, Claudia Andrea Olivera, Valeria Soledad Vagnozzi, Ariel Eduardo Perez, Andrés M. Konig, Guido Alberto |
| author_sort | Craig, María Isabel |
| collection | INTA Digital |
| description | Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country. |
| format | info:ar-repo/semantics/artículo |
| id | INTA2214 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA22142018-04-10T14:55:30Z Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina Craig, María Isabel Rojas, María Florencia Van Der Ploeg, Claudia Andrea Olivera, Valeria Soledad Vagnozzi, Ariel Eduardo Perez, Andrés M. Konig, Guido Alberto Laringotraqueitis Virus Bronquitis Infecciosa Aviar Virus de los Animales Epidemiología Glicoproteínas Genética Molecular Molecular Genetics Glycoproteins Epidemiology Animal Viruses Avian Infectious Bronchitis Virus Laryngotracheitis Infectious Laryngotracheitis Virus Spatial Cluster Analysis Análisis de Cluster Espacial Argentina Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country. Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; Argentina Fil: Van Der Ploeg, Claudia Andrea. Instituto Nacional de Enfermedades Infecciosas “Dr C. G. Malbrán”; Argentina Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Perez, Andrés M. University of Minnesota. College of Veterinary Medicine. Department of Veterinary Population Medicine; Estados Unidos Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-04-10T14:41:14Z 2018-04-10T14:41:14Z 2017-12-13 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2214 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733342/ 2297-1769 10.3389/fvets.2017.00212 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina (nation) Frontiers in veterinary science 4. (2017) |
| spellingShingle | Laringotraqueitis Virus Bronquitis Infecciosa Aviar Virus de los Animales Epidemiología Glicoproteínas Genética Molecular Molecular Genetics Glycoproteins Epidemiology Animal Viruses Avian Infectious Bronchitis Virus Laryngotracheitis Infectious Laryngotracheitis Virus Spatial Cluster Analysis Análisis de Cluster Espacial Argentina Craig, María Isabel Rojas, María Florencia Van Der Ploeg, Claudia Andrea Olivera, Valeria Soledad Vagnozzi, Ariel Eduardo Perez, Andrés M. Konig, Guido Alberto Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina |
| title | Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina |
| title_full | Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina |
| title_fullStr | Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina |
| title_full_unstemmed | Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina |
| title_short | Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina |
| title_sort | molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from argentina |
| topic | Laringotraqueitis Virus Bronquitis Infecciosa Aviar Virus de los Animales Epidemiología Glicoproteínas Genética Molecular Molecular Genetics Glycoproteins Epidemiology Animal Viruses Avian Infectious Bronchitis Virus Laryngotracheitis Infectious Laryngotracheitis Virus Spatial Cluster Analysis Análisis de Cluster Espacial Argentina |
| url | http://hdl.handle.net/20.500.12123/2214 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733342/ |
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