Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets
ZAT (zinc finger of Arabidopsis thaliana) proteins play roles in multiple abiotic stress tolerance in Arabidopsis and other species; however, there are few reports on these transcription factors (TFs) in fruit crops. This study aimed to evaluate PpZAT12, a C2H2 TF up-regulated in peach fruit by a he...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/22107 https://link.springer.com/article/10.1007/s00425-024-04593-x https://doi.org/10.1007/s00425-024-04593-x |
| _version_ | 1855038517604777984 |
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| author | Gismondi, Mauro Strologo, Laura Gabilondo, Julieta Budde, Claudio Olaf Drincovich, María Fabiana Bustamante, Claudia Anabel |
| author_browse | Budde, Claudio Olaf Bustamante, Claudia Anabel Drincovich, María Fabiana Gabilondo, Julieta Gismondi, Mauro Strologo, Laura |
| author_facet | Gismondi, Mauro Strologo, Laura Gabilondo, Julieta Budde, Claudio Olaf Drincovich, María Fabiana Bustamante, Claudia Anabel |
| author_sort | Gismondi, Mauro |
| collection | INTA Digital |
| description | ZAT (zinc finger of Arabidopsis thaliana) proteins play roles in multiple abiotic stress tolerance in Arabidopsis and other species; however, there are few reports on these transcription factors (TFs) in fruit crops. This study aimed to evaluate PpZAT12, a C2H2 TF up-regulated in peach fruit by a heat treatment applied before postharvest cold storage for reducing chilling injury (CI) symptoms. Here, the expression of PpZAT12 in different tissues and fruits subjected to either postharvest heat or cold treatments, was evaluated in peach varieties with differential susceptibility to develop CI. PpZAT12 increased by cold storage in CI-resistant cultivars (‘Elegant Lady’ and ‘Rojo 2’), while it was not modified in a cultivar susceptible to develop CI (‘Flordaking’). Besides, we expressed PpZAT12 in Arabidopsis (35S::PpZAT12) and found that these plants show impaired plant growth and development, rendering small plants with senescence delay and aborted seeds. We applied a proteomic approach to decipher the peptides responding to PpZAT12 in Arabidopsis and found 348 differential expressed proteins (DEPs) relative to the wild type. Besides, comparing the DEPs between Arabidopsis plants expressing PpZAT12 or AtZAT12 (35S::AtZAT12) we found common and specific responses to these TFs. Based on the proteomic information obtained here and published data about AtZAT12, we searched ZAT12-targets in peach allowing the identification of a putative ZAT12 regulon in this species. The identified peach ZAT12-protein targets could underlie the differential susceptibility to CI among different peach varieties and can be used as future targets to improve adaptation to refrigeration in fleshy fruits. |
| format | info:ar-repo/semantics/artículo |
| id | INTA22107 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA221072025-04-29T16:40:23Z Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets Gismondi, Mauro Strologo, Laura Gabilondo, Julieta Budde, Claudio Olaf Drincovich, María Fabiana Bustamante, Claudia Anabel Prunus persica Peaches Arabidopsis thaliana Cooling Genetics Resistance to Injurious Factors Cold Tolerance Durazno Enfriamiento Genética Resistencia a Agentes Dañinos Tolerancia al Frío Chilling ZAT12 ZAT (zinc finger of Arabidopsis thaliana) proteins play roles in multiple abiotic stress tolerance in Arabidopsis and other species; however, there are few reports on these transcription factors (TFs) in fruit crops. This study aimed to evaluate PpZAT12, a C2H2 TF up-regulated in peach fruit by a heat treatment applied before postharvest cold storage for reducing chilling injury (CI) symptoms. Here, the expression of PpZAT12 in different tissues and fruits subjected to either postharvest heat or cold treatments, was evaluated in peach varieties with differential susceptibility to develop CI. PpZAT12 increased by cold storage in CI-resistant cultivars (‘Elegant Lady’ and ‘Rojo 2’), while it was not modified in a cultivar susceptible to develop CI (‘Flordaking’). Besides, we expressed PpZAT12 in Arabidopsis (35S::PpZAT12) and found that these plants show impaired plant growth and development, rendering small plants with senescence delay and aborted seeds. We applied a proteomic approach to decipher the peptides responding to PpZAT12 in Arabidopsis and found 348 differential expressed proteins (DEPs) relative to the wild type. Besides, comparing the DEPs between Arabidopsis plants expressing PpZAT12 or AtZAT12 (35S::AtZAT12) we found common and specific responses to these TFs. Based on the proteomic information obtained here and published data about AtZAT12, we searched ZAT12-targets in peach allowing the identification of a putative ZAT12 regulon in this species. The identified peach ZAT12-protein targets could underlie the differential susceptibility to CI among different peach varieties and can be used as future targets to improve adaptation to refrigeration in fleshy fruits. EEA San Pedro Fil: Gismondi, Mauro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Strologo, Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Bustamante, Claudia A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-04-29T16:36:57Z 2025-04-29T16:36:57Z 2025-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22107 https://link.springer.com/article/10.1007/s00425-024-04593-x 0032-0935 1432-2048 https://doi.org/10.1007/s00425-024-04593-x eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Planta 261 : article number 14. (January 2025) |
| spellingShingle | Prunus persica Peaches Arabidopsis thaliana Cooling Genetics Resistance to Injurious Factors Cold Tolerance Durazno Enfriamiento Genética Resistencia a Agentes Dañinos Tolerancia al Frío Chilling ZAT12 Gismondi, Mauro Strologo, Laura Gabilondo, Julieta Budde, Claudio Olaf Drincovich, María Fabiana Bustamante, Claudia Anabel Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets |
| title | Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets |
| title_full | Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets |
| title_fullStr | Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets |
| title_full_unstemmed | Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets |
| title_short | Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets |
| title_sort | characterization of zat12 protein from prunus persica role in fruit chilling injury tolerance and identification of gene targets |
| topic | Prunus persica Peaches Arabidopsis thaliana Cooling Genetics Resistance to Injurious Factors Cold Tolerance Durazno Enfriamiento Genética Resistencia a Agentes Dañinos Tolerancia al Frío Chilling ZAT12 |
| url | http://hdl.handle.net/20.500.12123/22107 https://link.springer.com/article/10.1007/s00425-024-04593-x https://doi.org/10.1007/s00425-024-04593-x |
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