Combining linkage and association mapping approaches to study the genetic architecture of verticillium wilt resistance in sunflower
Sunflower Verticillium Wilt and Leaf Mottle (SVW), caused by Verticillium dahliae Kleb., is a globally prevalent disease affecting sunflower production. In this study, we identified a major quantitative trait locus (QTL) on chromosome 10 and other genomic regions associated with SVW resistance by in...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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MDPI
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/22102 https://www.mdpi.com/2223-7747/14/8/1187 https://doi.org/10.3390/plants14081187 |
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| author | Montecchia, Juan Francisco Fass, Monica Irinia Dominguez, Matías Gonzalez, Sergio Alberto Garcia, Martin Nahuel Filippi, Carla Valeria Ben Guerrero, Emiliano Maringolo, Carla Andrea Troglia, Carolina Beatriz Quiroz, Facundo Jose Gonzalez, Julio Horacio Alvarez, Daniel Heinz, Ruth Lia, Veronica Viviana Paniego, Norma Beatriz |
| author_browse | Alvarez, Daniel Ben Guerrero, Emiliano Dominguez, Matías Fass, Monica Irinia Filippi, Carla Valeria Garcia, Martin Nahuel Gonzalez, Julio Horacio Gonzalez, Sergio Alberto Heinz, Ruth Lia, Veronica Viviana Maringolo, Carla Andrea Montecchia, Juan Francisco Paniego, Norma Beatriz Quiroz, Facundo Jose Troglia, Carolina Beatriz |
| author_facet | Montecchia, Juan Francisco Fass, Monica Irinia Dominguez, Matías Gonzalez, Sergio Alberto Garcia, Martin Nahuel Filippi, Carla Valeria Ben Guerrero, Emiliano Maringolo, Carla Andrea Troglia, Carolina Beatriz Quiroz, Facundo Jose Gonzalez, Julio Horacio Alvarez, Daniel Heinz, Ruth Lia, Veronica Viviana Paniego, Norma Beatriz |
| author_sort | Montecchia, Juan Francisco |
| collection | INTA Digital |
| description | Sunflower Verticillium Wilt and Leaf Mottle (SVW), caused by Verticillium dahliae Kleb., is a globally prevalent disease affecting sunflower production. In this study, we identified a major quantitative trait locus (QTL) on chromosome 10 and other genomic regions associated with SVW resistance by integrating biparental and association mapping in sunflower populations from the National Institute of Agricultural Technology. Nine replicated field trials were conducted in highly infested V. dahliae reservoirs to assess disease incidence and severity. Both mapping populations were genotyped using double-digest restriction-site-associated DNA sequencing (ddRADseq). Association mapping with 18,161 SNPs and biparental QTL mapping with 1769 SNPs identified a major QTL on chromosome 10 explaining up to 30% of phenotypic variation for disease incidence at flowering and for the area under the disease progress curve for disease incidence, and which contributes to a lesser extent to disease severity reduction. Additional QTLs on chromosomes 17, 8, 9, 14, 13, and 11 were associated with reduced disease incidence, severity, or both. Candidate genes were identified within these associated regions, 39 of which are in the major QTL on Chromosome 10. These findings demonstrate the value of integrating complementary QTL mapping strategies for validating resistance loci and advancing sunflower breeding for SVW resistance. |
| format | info:ar-repo/semantics/artículo |
| id | INTA22102 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA221022025-04-29T14:43:33Z Combining linkage and association mapping approaches to study the genetic architecture of verticillium wilt resistance in sunflower Montecchia, Juan Francisco Fass, Monica Irinia Dominguez, Matías Gonzalez, Sergio Alberto Garcia, Martin Nahuel Filippi, Carla Valeria Ben Guerrero, Emiliano Maringolo, Carla Andrea Troglia, Carolina Beatriz Quiroz, Facundo Jose Gonzalez, Julio Horacio Alvarez, Daniel Heinz, Ruth Lia, Veronica Viviana Paniego, Norma Beatriz Verticillium dahliae Quantitative Trait Loci Mapping Cartografía de Loci de Rasgos Cuantitativos Estudio de Asociación del Genoma Completo Resistencia a la Enfermedad Girasol Secuencia de ADN Genome-wide Association Studies Disease Resistance Sunflowers DNA Sequences Sunflower Verticillium Wilt and Leaf Mottle (SVW), caused by Verticillium dahliae Kleb., is a globally prevalent disease affecting sunflower production. In this study, we identified a major quantitative trait locus (QTL) on chromosome 10 and other genomic regions associated with SVW resistance by integrating biparental and association mapping in sunflower populations from the National Institute of Agricultural Technology. Nine replicated field trials were conducted in highly infested V. dahliae reservoirs to assess disease incidence and severity. Both mapping populations were genotyped using double-digest restriction-site-associated DNA sequencing (ddRADseq). Association mapping with 18,161 SNPs and biparental QTL mapping with 1769 SNPs identified a major QTL on chromosome 10 explaining up to 30% of phenotypic variation for disease incidence at flowering and for the area under the disease progress curve for disease incidence, and which contributes to a lesser extent to disease severity reduction. Additional QTLs on chromosomes 17, 8, 9, 14, 13, and 11 were associated with reduced disease incidence, severity, or both. Candidate genes were identified within these associated regions, 39 of which are in the major QTL on Chromosome 10. These findings demonstrate the value of integrating complementary QTL mapping strategies for validating resistance loci and advancing sunflower breeding for SVW resistance. Instituto de Biotecnología Fil: Montecchia, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Montecchia, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Montecchia, Juan Francisco. Advanta Semillas SAIC. Estación Experimental Venado Tuerto; Argentina Fil: Fass, Mónica Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Fass, Mónica Irina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dominguez, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina Fil: González, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: González, Sergio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garcia, Martin Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Garcia, Martin Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Filippi, Carla Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Filippi, Carla Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Filippi, Carla Valeria. Universidad de la República. Facultad de Agronomía. Departamento de Biología Vegetal. Laboratorio de Bioquímica; Uruguay Fil: Ben Guerrero, Emiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ben Guerrero, Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Maringolo, Carla Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Troglia, Carolina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Quiroz, Facundo Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Gonzalez, Julio Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina Fil: Alvarez, Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Heinz, Ruth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Heinz, Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lia, Verónica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Lia, Verónica Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lia, Verónica Viviana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Paniego, Norma Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-04-29T14:31:44Z 2025-04-29T14:31:44Z 2025-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22102 https://www.mdpi.com/2223-7747/14/8/1187 2223-7747 https://doi.org/10.3390/plants14081187 eng info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud info:eu-repograntAgreement/INTA/2019-PE-E6-I127-001, Mejoramiento genético de soja, girasol y oleaginosas invernales en calidad y valor agregado, productividad, y estabilidad y plasticidad ante estreses bióticos y abióticos info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Plants 14 (8) : 1187 (April 2025) |
| spellingShingle | Verticillium dahliae Quantitative Trait Loci Mapping Cartografía de Loci de Rasgos Cuantitativos Estudio de Asociación del Genoma Completo Resistencia a la Enfermedad Girasol Secuencia de ADN Genome-wide Association Studies Disease Resistance Sunflowers DNA Sequences Montecchia, Juan Francisco Fass, Monica Irinia Dominguez, Matías Gonzalez, Sergio Alberto Garcia, Martin Nahuel Filippi, Carla Valeria Ben Guerrero, Emiliano Maringolo, Carla Andrea Troglia, Carolina Beatriz Quiroz, Facundo Jose Gonzalez, Julio Horacio Alvarez, Daniel Heinz, Ruth Lia, Veronica Viviana Paniego, Norma Beatriz Combining linkage and association mapping approaches to study the genetic architecture of verticillium wilt resistance in sunflower |
| title | Combining linkage and association mapping approaches to study the genetic architecture of verticillium wilt resistance in sunflower |
| title_full | Combining linkage and association mapping approaches to study the genetic architecture of verticillium wilt resistance in sunflower |
| title_fullStr | Combining linkage and association mapping approaches to study the genetic architecture of verticillium wilt resistance in sunflower |
| title_full_unstemmed | Combining linkage and association mapping approaches to study the genetic architecture of verticillium wilt resistance in sunflower |
| title_short | Combining linkage and association mapping approaches to study the genetic architecture of verticillium wilt resistance in sunflower |
| title_sort | combining linkage and association mapping approaches to study the genetic architecture of verticillium wilt resistance in sunflower |
| topic | Verticillium dahliae Quantitative Trait Loci Mapping Cartografía de Loci de Rasgos Cuantitativos Estudio de Asociación del Genoma Completo Resistencia a la Enfermedad Girasol Secuencia de ADN Genome-wide Association Studies Disease Resistance Sunflowers DNA Sequences |
| url | http://hdl.handle.net/20.500.12123/22102 https://www.mdpi.com/2223-7747/14/8/1187 https://doi.org/10.3390/plants14081187 |
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