Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages
Macrophages are important cells of the innate immunity that play a major role in Bovine Viral Diarrhoea Virus (BVDV) pathogenesis. Macrophages are not a homogenous population; they exist in different phenotypes, typically divided into two main categories: classically (pro-inflammatory) and alternati...
| Main Authors: | , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Taylor & Francis
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/22064 https://www.tandfonline.com/doi/full/10.1080/21505594.2023.2283899 https://doi.org/10.1080/21505594.2023.2283899 |
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| author | Barone, Lucas Jose Cardoso, Nancy Mansilla, Florencia Celeste Castillo, Mariangeles Capozzo, Alejandra |
| author_browse | Barone, Lucas Jose Capozzo, Alejandra Cardoso, Nancy Castillo, Mariangeles Mansilla, Florencia Celeste |
| author_facet | Barone, Lucas Jose Cardoso, Nancy Mansilla, Florencia Celeste Castillo, Mariangeles Capozzo, Alejandra |
| author_sort | Barone, Lucas Jose |
| collection | INTA Digital |
| description | Macrophages are important cells of the innate immunity that play a major role in Bovine Viral Diarrhoea Virus (BVDV) pathogenesis. Macrophages are not a homogenous population; they exist in different phenotypes, typically divided into two main categories: classically (pro-inflammatory) and alternatively activated (anti-inflammatory) or M1 and M2, respectively. The role of bovine macrophage phenotypes on BVDV infection is still unclear. This study characterized the interaction between BVDV and monocyte-derived macrophages (Mo-Mφ) collected from healthy cattle and polarized to an M1 or M2 state by using LPS, INF-γ, IL-4, or azithromycin. Arginase activity quantitation was utilized as a marker of the M2 Mo-Mφ spectrum. There was a significant association between arginase activity and the replication rate of BVDV strains of different genotypes and biotypes. Inhibition of arginase activity also reduced BVDV infectivity. Calves treated with azithromycin-induced Mo-Mφ of the M2 state produced high levels of arginase. Interestingly, azithromycin administered in vivo increased the susceptibility of macrophages to BVDV infection ex vivo. Mo-Mφ from pregnant dams and calves produced higher arginase levels than those from non-pregnant adult animals. The increased infection of arginase-producing alternatively activated bovine macrophages with BVDV supports the need to delve into a possible leading role of M2 macrophages in establishing the immune-suppressive state during BVDV convalescence. |
| format | info:ar-repo/semantics/artículo |
| id | INTA22064 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Taylor & Francis |
| publisherStr | Taylor & Francis |
| record_format | dspace |
| spelling | INTA220642025-04-28T10:10:28Z Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages Barone, Lucas Jose Cardoso, Nancy Mansilla, Florencia Celeste Castillo, Mariangeles Capozzo, Alejandra Macrophages Pestivirus Immunosuppression Replication Bovine Viral Diarrhoea Monocytes Macrófago Inmunodepresión Replicación Diarrea Viral Bovina Monocito Azithromycin Azitromicina Macrophages are important cells of the innate immunity that play a major role in Bovine Viral Diarrhoea Virus (BVDV) pathogenesis. Macrophages are not a homogenous population; they exist in different phenotypes, typically divided into two main categories: classically (pro-inflammatory) and alternatively activated (anti-inflammatory) or M1 and M2, respectively. The role of bovine macrophage phenotypes on BVDV infection is still unclear. This study characterized the interaction between BVDV and monocyte-derived macrophages (Mo-Mφ) collected from healthy cattle and polarized to an M1 or M2 state by using LPS, INF-γ, IL-4, or azithromycin. Arginase activity quantitation was utilized as a marker of the M2 Mo-Mφ spectrum. There was a significant association between arginase activity and the replication rate of BVDV strains of different genotypes and biotypes. Inhibition of arginase activity also reduced BVDV infectivity. Calves treated with azithromycin-induced Mo-Mφ of the M2 state produced high levels of arginase. Interestingly, azithromycin administered in vivo increased the susceptibility of macrophages to BVDV infection ex vivo. Mo-Mφ from pregnant dams and calves produced higher arginase levels than those from non-pregnant adult animals. The increased infection of arginase-producing alternatively activated bovine macrophages with BVDV supports the need to delve into a possible leading role of M2 macrophages in establishing the immune-suppressive state during BVDV convalescence. Instituto de Virología Fil: Barone, Lucas Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Barone, Lucas Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Cardoso, Nancy Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Mansilla, Florencia Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Castillo, Mariangeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Castillo, Mariangeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Capozzo, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-04-28T10:04:35Z 2025-04-28T10:04:35Z 2024 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22064 https://www.tandfonline.com/doi/full/10.1080/21505594.2023.2283899 2150-5608 https://doi.org/10.1080/21505594.2023.2283899 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Taylor & Francis Virulence 15 (1) : 2283899 (2024) |
| spellingShingle | Macrophages Pestivirus Immunosuppression Replication Bovine Viral Diarrhoea Monocytes Macrófago Inmunodepresión Replicación Diarrea Viral Bovina Monocito Azithromycin Azitromicina Barone, Lucas Jose Cardoso, Nancy Mansilla, Florencia Celeste Castillo, Mariangeles Capozzo, Alejandra Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages |
| title | Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages |
| title_full | Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages |
| title_fullStr | Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages |
| title_full_unstemmed | Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages |
| title_short | Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages |
| title_sort | enhanced infectivity of bovine viral diarrhoea virus bvdv in arginase producing bovine monocyte derived macrophages |
| topic | Macrophages Pestivirus Immunosuppression Replication Bovine Viral Diarrhoea Monocytes Macrófago Inmunodepresión Replicación Diarrea Viral Bovina Monocito Azithromycin Azitromicina |
| url | http://hdl.handle.net/20.500.12123/22064 https://www.tandfonline.com/doi/full/10.1080/21505594.2023.2283899 https://doi.org/10.1080/21505594.2023.2283899 |
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