Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting
Background/Objectives: Vaccines may improve the control and eradication of bovine tuberculosis. However, the evaluation of experimental candidates requires the assessment of the protection, excretion, transmission and biosafety. A natural transmission trial among likely infected animals was conducte...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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MDPI
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/22047 https://www.mdpi.com/2076-393X/12/10/1173 https://doi.org/10.3390/vaccines12101173 |
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| author | Ferrara Muñiz, Ximena Garcia, Elizabeth Andrea Blanco, Federico Carlos Garbaccio, Sergio Gabriel Garro, Carlos Javier Zumarraga, Martin Jose Dellagostin, Odir A. Trangoni, Marcos David Marfil, Maria Jimena Bianco, María Veronica Abdala, Alejandro Ariel Revelli, Javier Bergamasco, Maria Soutullo, Adriana Marini, M. Rocío Rocha, Rosana Valeria Sánchez, Amorina Bigi, Fabiana Canal, Ana María Eirin, Maria Emilia Cataldi, Angel Adrian |
| author_browse | Abdala, Alejandro Ariel Bergamasco, Maria Bianco, María Veronica Bigi, Fabiana Blanco, Federico Carlos Canal, Ana María Cataldi, Angel Adrian Dellagostin, Odir A. Eirin, Maria Emilia Ferrara Muñiz, Ximena Garbaccio, Sergio Gabriel Garcia, Elizabeth Andrea Garro, Carlos Javier Marfil, Maria Jimena Marini, M. Rocío Revelli, Javier Rocha, Rosana Valeria Soutullo, Adriana Sánchez, Amorina Trangoni, Marcos David Zumarraga, Martin Jose |
| author_facet | Ferrara Muñiz, Ximena Garcia, Elizabeth Andrea Blanco, Federico Carlos Garbaccio, Sergio Gabriel Garro, Carlos Javier Zumarraga, Martin Jose Dellagostin, Odir A. Trangoni, Marcos David Marfil, Maria Jimena Bianco, María Veronica Abdala, Alejandro Ariel Revelli, Javier Bergamasco, Maria Soutullo, Adriana Marini, M. Rocío Rocha, Rosana Valeria Sánchez, Amorina Bigi, Fabiana Canal, Ana María Eirin, Maria Emilia Cataldi, Angel Adrian |
| author_sort | Ferrara Muñiz, Ximena |
| collection | INTA Digital |
| description | Background/Objectives: Vaccines may improve the control and eradication of bovine tuberculosis. However, the evaluation of experimental candidates requires the assessment of the protection, excretion, transmission and biosafety. A natural transmission trial among likely infected animals was conducted. Methods: Seventy-four male heifers were randomly distributed (five groups) and vaccinated subcutaneously with attenuated strains (M. bovis Δmce2 or M. bovis Δmce2-phoP), a recombinant M. bovis BCG Pasteur (BCGr) or M. bovis BCG Pasteur. Then, they cohoused with a naturally infected bTB cohort under field conditions exposed to the infection. Results: A 23% of transmission of wild-type strains was confirmed (non-vaccinated group). Strikingly, first vaccination did not induce immune response (caudal fold test and IFN-gamma release assay). However, after 74 days of exposure to bTB, animals were re-vaccinated. Although their sensitization increased throughout the trial, the vaccines did not confer significant protection, when compared to the non-vaccinated group, as demonstrated by pathology progression of lesions and confirmatory tools. Besides, the likelihood of acquiring the infection was similar in all groups compared to the non-vaccinated group (p > 0.076). Respiratory and digestive excretion of viable vaccine candidates was undetectable. To note, the group vaccinated with M. bovis Δmce2-phoP exhibited the highest proportion of animals without macroscopic lesions, compared to the one vaccinated with BCG, although this was not statistically supported. Conclusions: This highlights that further evaluation of these vaccines would not guarantee better protection. The limitations detected during the trial are discussed regarding the transmission rate of M. bovis wild-type, the imperfect test for studying sensitization, the need for a DIVA diagnosis and management conditions of the trials performed under routine husbandry conditions. Re-vaccination of likely infected bovines did not highlight a conclusive result, even suggesting a detrimental effect on those vaccinated with M. bovis BCG. |
| format | Artículo |
| id | INTA22047 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA220472025-04-24T15:58:52Z Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting Ferrara Muñiz, Ximena Garcia, Elizabeth Andrea Blanco, Federico Carlos Garbaccio, Sergio Gabriel Garro, Carlos Javier Zumarraga, Martin Jose Dellagostin, Odir A. Trangoni, Marcos David Marfil, Maria Jimena Bianco, María Veronica Abdala, Alejandro Ariel Revelli, Javier Bergamasco, Maria Soutullo, Adriana Marini, M. Rocío Rocha, Rosana Valeria Sánchez, Amorina Bigi, Fabiana Canal, Ana María Eirin, Maria Emilia Cataldi, Angel Adrian Bovine Tuberculosis Bovinae Vaccines Live Vaccines Field Experimentation Tuberculosis Bovina Vacuna Vacuna Viva Experimentación en Campo Background/Objectives: Vaccines may improve the control and eradication of bovine tuberculosis. However, the evaluation of experimental candidates requires the assessment of the protection, excretion, transmission and biosafety. A natural transmission trial among likely infected animals was conducted. Methods: Seventy-four male heifers were randomly distributed (five groups) and vaccinated subcutaneously with attenuated strains (M. bovis Δmce2 or M. bovis Δmce2-phoP), a recombinant M. bovis BCG Pasteur (BCGr) or M. bovis BCG Pasteur. Then, they cohoused with a naturally infected bTB cohort under field conditions exposed to the infection. Results: A 23% of transmission of wild-type strains was confirmed (non-vaccinated group). Strikingly, first vaccination did not induce immune response (caudal fold test and IFN-gamma release assay). However, after 74 days of exposure to bTB, animals were re-vaccinated. Although their sensitization increased throughout the trial, the vaccines did not confer significant protection, when compared to the non-vaccinated group, as demonstrated by pathology progression of lesions and confirmatory tools. Besides, the likelihood of acquiring the infection was similar in all groups compared to the non-vaccinated group (p > 0.076). Respiratory and digestive excretion of viable vaccine candidates was undetectable. To note, the group vaccinated with M. bovis Δmce2-phoP exhibited the highest proportion of animals without macroscopic lesions, compared to the one vaccinated with BCG, although this was not statistically supported. Conclusions: This highlights that further evaluation of these vaccines would not guarantee better protection. The limitations detected during the trial are discussed regarding the transmission rate of M. bovis wild-type, the imperfect test for studying sensitization, the need for a DIVA diagnosis and management conditions of the trials performed under routine husbandry conditions. Re-vaccination of likely infected bovines did not highlight a conclusive result, even suggesting a detrimental effect on those vaccinated with M. bovis BCG. Instituto de Biotecnología Fil: Ferrara Muñiz, Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ferrara Muñiz, Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Garcia, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Garbaccio, Sergio Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Garro, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dellagostin, Odir A.. Universidade Federal de Pelotas. Centro de Desenvolvimento Tecnológico. Núcleo de Biotecnología; Brasil Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marfil, Maria Jimena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas; Argentina Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigaciones Agropecuarias (CIAP). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Revelli, Javier. Actividad privada; Argentina Fil: Bergamasco, Maria. Ministerio de Desarrollo Productivo de Santa Fe. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; Argentina Fil: Soutullo, Adriana. Universidad Nacional del Litoral. Facultad de Ciencias Bioquímicas y Biológicas. Cátedra de Inmunología Básica; Argentina Fil: Marini, M. Rocío. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Veterinaria; Argentina Fil: Rocha, Rosana Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Rocha, Rosana Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sánchez, Amorina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Veterinaria; Argentina Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Bigi, Fabiana. Consejo Nacional de investigaciones Científicas y Técnicas; Argentina Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Veterinaria; Argentina Fil: Eirin, Maria Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Eirin, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-04-24T15:08:09Z 2025-04-24T15:08:09Z 2024-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/22047 https://www.mdpi.com/2076-393X/12/10/1173 2076-393X https://doi.org/10.3390/vaccines12101173 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001, Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud? info:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Salud info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Vaccines 12 (10) : 1173 (October 2024) |
| spellingShingle | Bovine Tuberculosis Bovinae Vaccines Live Vaccines Field Experimentation Tuberculosis Bovina Vacuna Vacuna Viva Experimentación en Campo Ferrara Muñiz, Ximena Garcia, Elizabeth Andrea Blanco, Federico Carlos Garbaccio, Sergio Gabriel Garro, Carlos Javier Zumarraga, Martin Jose Dellagostin, Odir A. Trangoni, Marcos David Marfil, Maria Jimena Bianco, María Veronica Abdala, Alejandro Ariel Revelli, Javier Bergamasco, Maria Soutullo, Adriana Marini, M. Rocío Rocha, Rosana Valeria Sánchez, Amorina Bigi, Fabiana Canal, Ana María Eirin, Maria Emilia Cataldi, Angel Adrian Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| title | Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| title_full | Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| title_fullStr | Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| title_full_unstemmed | Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| title_short | Field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| title_sort | field trial with vaccine candidates against bovine tuberculosis among likely infected cattle in a natural transmission setting |
| topic | Bovine Tuberculosis Bovinae Vaccines Live Vaccines Field Experimentation Tuberculosis Bovina Vacuna Vacuna Viva Experimentación en Campo |
| url | http://hdl.handle.net/20.500.12123/22047 https://www.mdpi.com/2076-393X/12/10/1173 https://doi.org/10.3390/vaccines12101173 |
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