Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1

Whey permeate (WP), a by-product of cheese whey treatment, constitutes a sustainable and economical alternative to synthetic media for probiotic biomass production. Likewise, spray drying (SD) is a rapid and cost-effective alternative to freeze drying in industrial practice. This study aimed to opti...

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Main Authors: Zacarias, Maria Florencia, Cuatrin, Alejandra, Lavari, Luisina, Taverna, Miguel Angel, Paez, Roxana Beatriz, Vinderola, Celso Gabriel
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Elsevier 2025
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/21956
https://www.sciencedirect.com/science/article/abs/pii/S0958694624003017
https://doi.org/10.1016/j.idairyj.2024.106181
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author Zacarias, Maria Florencia
Cuatrin, Alejandra
Lavari, Luisina
Taverna, Miguel Angel
Paez, Roxana Beatriz
Vinderola, Celso Gabriel
author_browse Cuatrin, Alejandra
Lavari, Luisina
Paez, Roxana Beatriz
Taverna, Miguel Angel
Vinderola, Celso Gabriel
Zacarias, Maria Florencia
author_facet Zacarias, Maria Florencia
Cuatrin, Alejandra
Lavari, Luisina
Taverna, Miguel Angel
Paez, Roxana Beatriz
Vinderola, Celso Gabriel
author_sort Zacarias, Maria Florencia
collection INTA Digital
description Whey permeate (WP), a by-product of cheese whey treatment, constitutes a sustainable and economical alternative to synthetic media for probiotic biomass production. Likewise, spray drying (SD) is a rapid and cost-effective alternative to freeze drying in industrial practice. This study aimed to optimise the production of a spray-dried culture (SDC) of Bifidobacterium animalis subsp. lactis INL1. Biomass production on WP was optimised by analyzing the effects of yeast extract and pH on bacterial growth, survival during spray drying, and resistance to simulated gastric digestion. Additionally, pH, inlet temperature, and drying matrix effects were evaluated for their impact on strain resistance during SD and storage. WP proved to be a suitable ingredient for B. lactis INL1 biomass production. Acidic culture conditions yielded superior results, and all tested factors significantly influenced SDC performance. These findings highlight the usefulness of optimization strategies to define effective production conditions for probiotic cultures.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2025
publishDateRange 2025
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publisherStr Elsevier
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spelling INTA219562025-04-08T13:46:40Z Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1 Zacarias, Maria Florencia Cuatrin, Alejandra Lavari, Luisina Taverna, Miguel Angel Paez, Roxana Beatriz Vinderola, Celso Gabriel Biomasa Lactosuero Probióticos Secado por Pulverización Biomass Whey Bifidobacterium Probiotics Spray Drying Bifidobacterium animalis subsp. lactis Whey permeate (WP), a by-product of cheese whey treatment, constitutes a sustainable and economical alternative to synthetic media for probiotic biomass production. Likewise, spray drying (SD) is a rapid and cost-effective alternative to freeze drying in industrial practice. This study aimed to optimise the production of a spray-dried culture (SDC) of Bifidobacterium animalis subsp. lactis INL1. Biomass production on WP was optimised by analyzing the effects of yeast extract and pH on bacterial growth, survival during spray drying, and resistance to simulated gastric digestion. Additionally, pH, inlet temperature, and drying matrix effects were evaluated for their impact on strain resistance during SD and storage. WP proved to be a suitable ingredient for B. lactis INL1 biomass production. Acidic culture conditions yielded superior results, and all tested factors significantly influenced SDC performance. These findings highlight the usefulness of optimization strategies to define effective production conditions for probiotic cultures. EEA Rafaela Fil: Zacarías, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Zacarías, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cuatrin, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Lavari, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Lavari, Luisina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Taverna, Miguel Angel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Paez, Roxana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Lactología Industrial; Argentina Fil: Vinderola, Celso Gabriel. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-04-08T13:40:02Z 2025-04-08T13:40:02Z 2025-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21956 https://www.sciencedirect.com/science/article/abs/pii/S0958694624003017 0958-6946 1879-0143 https://doi.org/10.1016/j.idairyj.2024.106181 eng info:eu-repograntAgreement/INTA/PNAIyAV-1130032/AR./Tecnologías de transformación de alimentos. info:eu-repograntAgreement/INTA/PNAIyAV-1130033/AR./Tecnologías de preservación de alimentos y de aprovechamiento de subproductos. info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier International Dairy Journal 163 : 106181. (April 2025)
spellingShingle Biomasa
Lactosuero
Probióticos
Secado por Pulverización
Biomass
Whey
Bifidobacterium
Probiotics
Spray Drying
Bifidobacterium animalis subsp. lactis
Zacarias, Maria Florencia
Cuatrin, Alejandra
Lavari, Luisina
Taverna, Miguel Angel
Paez, Roxana Beatriz
Vinderola, Celso Gabriel
Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
title Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
title_full Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
title_fullStr Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
title_full_unstemmed Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
title_short Biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk, Bifidobacterium animalis subsp. lactis INL1
title_sort biomass production in whey permeate and spray drying of a potential probiotic strain from human breast milk bifidobacterium animalis subsp lactis inl1
topic Biomasa
Lactosuero
Probióticos
Secado por Pulverización
Biomass
Whey
Bifidobacterium
Probiotics
Spray Drying
Bifidobacterium animalis subsp. lactis
url http://hdl.handle.net/20.500.12123/21956
https://www.sciencedirect.com/science/article/abs/pii/S0958694624003017
https://doi.org/10.1016/j.idairyj.2024.106181
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