Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples
Leptospirosis is a significant infectious disease affecting livestock. Microscopic agglutination test (MAT) is used as the gold standard; however, the inclusion of molecular tools (PCR) could improve the accuracy of diagnosis. We evaluated the combination of MAT and a LigB-PCR in bovine serum to det...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/21952 https://www.sciencedirect.com/science/article/abs/pii/S0034528825000761 https://doi.org/10.1016/j.rvsc.2025.105602 |
| _version_ | 1855038489177882624 |
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| author | Saraullo, Vanina Rosa Hamer, Micaela Esteban, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila |
| author_browse | Brihuega, Bibiana Felicitas Esteban, Micaela Hamer, Micaela Martinez, Mara Leila Sanchez, Maria Cristina Saraullo, Vanina Rosa |
| author_facet | Saraullo, Vanina Rosa Hamer, Micaela Esteban, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila |
| author_sort | Saraullo, Vanina Rosa |
| collection | INTA Digital |
| description | Leptospirosis is a significant infectious disease affecting livestock. Microscopic agglutination test (MAT) is used as the gold standard; however, the inclusion of molecular tools (PCR) could improve the accuracy of diagnosis. We evaluated the combination of MAT and a LigB-PCR in bovine serum to detect positive cases in animals clinically suspected of leptospirosis. 127 samples were classified by MAT (cut-off 1:100). Of 45 negative samples negative by MAT, 42.2 % were positive by PCR. In addition, the number of confirmed cases of bovine leptospirosis based on clinical suspicion and MAT and/or PCR increased the diagnostic rate to 79.5 %. LigB-PCR can detect traces of leptospiral DNA in serum with negative or low antibody titers and could be used as a complementary tool to MAT. |
| format | info:ar-repo/semantics/artículo |
| id | INTA21952 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA219522025-04-08T12:53:08Z Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples Saraullo, Vanina Rosa Hamer, Micaela Esteban, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila PCR Leptospirosis Diagnosis Agglutination Tests Microscopy Bovinae Immunoenzyme Techniques Diagnóstico Reacción de Aglutinación Microscopia Técnica Inmunoenzimática Leptospirosis is a significant infectious disease affecting livestock. Microscopic agglutination test (MAT) is used as the gold standard; however, the inclusion of molecular tools (PCR) could improve the accuracy of diagnosis. We evaluated the combination of MAT and a LigB-PCR in bovine serum to detect positive cases in animals clinically suspected of leptospirosis. 127 samples were classified by MAT (cut-off 1:100). Of 45 negative samples negative by MAT, 42.2 % were positive by PCR. In addition, the number of confirmed cases of bovine leptospirosis based on clinical suspicion and MAT and/or PCR increased the diagnostic rate to 79.5 %. LigB-PCR can detect traces of leptospiral DNA in serum with negative or low antibody titers and could be used as a complementary tool to MAT. Instituto de Patobiología Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinez, Mara Leila. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina 2025-04-08T12:46:54Z 2025-04-08T12:46:54Z 2025-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21952 https://www.sciencedirect.com/science/article/abs/pii/S0034528825000761 1532-2661 https://doi.org/10.1016/j.rvsc.2025.105602 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Research in Veterinary Science 187 : 105602 (May 2025) |
| spellingShingle | PCR Leptospirosis Diagnosis Agglutination Tests Microscopy Bovinae Immunoenzyme Techniques Diagnóstico Reacción de Aglutinación Microscopia Técnica Inmunoenzimática Saraullo, Vanina Rosa Hamer, Micaela Esteban, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples |
| title | Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples |
| title_full | Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples |
| title_fullStr | Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples |
| title_full_unstemmed | Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples |
| title_short | Evaluation of LigB polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples |
| title_sort | evaluation of ligb polymerase chain reaction as a complementary tool to microscopic agglutination test for the detection of bovine leptospirosis in serum samples |
| topic | PCR Leptospirosis Diagnosis Agglutination Tests Microscopy Bovinae Immunoenzyme Techniques Diagnóstico Reacción de Aglutinación Microscopia Técnica Inmunoenzimática |
| url | http://hdl.handle.net/20.500.12123/21952 https://www.sciencedirect.com/science/article/abs/pii/S0034528825000761 https://doi.org/10.1016/j.rvsc.2025.105602 |
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