Redox regulation of autophagy in Arabidopsis: The different ROS effects
Autophagy plays a key role in the responses to different stress condition in plants. Reactive oxygen species (ROS) are common modulators of stress responses, having both toxic and signaling functions. In this context, the relationship between ROS and autophagy regulation remains unclear, and in some...
| Main Authors: | , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2025
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/21950 https://www.sciencedirect.com/science/article/abs/pii/S0981942825003286 https://doi.org/10.1016/j.plaphy.2025.109800 |
| _version_ | 1855038488980750336 |
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| author | Robert, German Enet, Alejandro Saavedra Borelli, Laura Lucia Lascano, Hernan Ramiro |
| author_browse | Enet, Alejandro Lascano, Hernan Ramiro Robert, German Saavedra Borelli, Laura Lucia |
| author_facet | Robert, German Enet, Alejandro Saavedra Borelli, Laura Lucia Lascano, Hernan Ramiro |
| author_sort | Robert, German |
| collection | INTA Digital |
| description | Autophagy plays a key role in the responses to different stress condition in plants. Reactive oxygen species (ROS) are common modulators of stress responses, having both toxic and signaling functions. In this context, the relationship between ROS and autophagy regulation remains unclear, and in some aspects, contradictory. In this study, we employed pharmacological and genetic approaches to investigate the effects of different ROS on the cytoplastic redox state and autophagic flux in Arabidopsis thaliana. Our results demonstrated that oxidative treatments with H 2 O 2 and MV, which drastically increased the oxidized state of the cytoplasm, reduced the autophagic flux. Conversely, singlet oxygen, which did not have significant effects on the cytoplasmic redox state, increased the autophagic flux. Additionally, our findings indicated that after H 2 O 2 and high light treatments and during the recovery period, the cytoplasm returned to its reduced state, while autophagy was markedly induced. In summary, our study unveils the differential effects of ROS on the autophagic flux, establishing a correlation with the redox state of the cytoplasm. Moreover, it emphasizes the dynamic nature of autophagy in response to oxidative stress and the subsequent recovery period. |
| format | info:ar-repo/semantics/artículo |
| id | INTA21950 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA219502025-04-08T12:44:02Z Redox regulation of autophagy in Arabidopsis: The different ROS effects Robert, German Enet, Alejandro Saavedra Borelli, Laura Lucia Lascano, Hernan Ramiro Stress Reactive Oxygen Species Hydrogen Peroxide Estres Especies de Oxígeno Reactivo Peróxido de Hidrógeno Arabidopsis thaliana Autophagy Singlet Oxygen Superoxide Radical Redox State Autophagy plays a key role in the responses to different stress condition in plants. Reactive oxygen species (ROS) are common modulators of stress responses, having both toxic and signaling functions. In this context, the relationship between ROS and autophagy regulation remains unclear, and in some aspects, contradictory. In this study, we employed pharmacological and genetic approaches to investigate the effects of different ROS on the cytoplastic redox state and autophagic flux in Arabidopsis thaliana. Our results demonstrated that oxidative treatments with H 2 O 2 and MV, which drastically increased the oxidized state of the cytoplasm, reduced the autophagic flux. Conversely, singlet oxygen, which did not have significant effects on the cytoplasmic redox state, increased the autophagic flux. Additionally, our findings indicated that after H 2 O 2 and high light treatments and during the recovery period, the cytoplasm returned to its reduced state, while autophagy was markedly induced. In summary, our study unveils the differential effects of ROS on the autophagic flux, establishing a correlation with the redox state of the cytoplasm. Moreover, it emphasizes the dynamic nature of autophagy in response to oxidative stress and the subsequent recovery period. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil:Robert, German. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Robert, German. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Enet, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Enet, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Saavedra Borelli, Laura Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Saavedra Borelli, Laura Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lascano, Hernan Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina 2025-04-08T12:37:40Z 2025-04-08T12:37:40Z 2025-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21950 https://www.sciencedirect.com/science/article/abs/pii/S0981942825003286 0981-9428 1873-2690 https://doi.org/10.1016/j.plaphy.2025.109800 eng info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Plant Physiology and Biochemistry 223 : 109800 (June 2025) |
| spellingShingle | Stress Reactive Oxygen Species Hydrogen Peroxide Estres Especies de Oxígeno Reactivo Peróxido de Hidrógeno Arabidopsis thaliana Autophagy Singlet Oxygen Superoxide Radical Redox State Robert, German Enet, Alejandro Saavedra Borelli, Laura Lucia Lascano, Hernan Ramiro Redox regulation of autophagy in Arabidopsis: The different ROS effects |
| title | Redox regulation of autophagy in Arabidopsis: The different ROS effects |
| title_full | Redox regulation of autophagy in Arabidopsis: The different ROS effects |
| title_fullStr | Redox regulation of autophagy in Arabidopsis: The different ROS effects |
| title_full_unstemmed | Redox regulation of autophagy in Arabidopsis: The different ROS effects |
| title_short | Redox regulation of autophagy in Arabidopsis: The different ROS effects |
| title_sort | redox regulation of autophagy in arabidopsis the different ros effects |
| topic | Stress Reactive Oxygen Species Hydrogen Peroxide Estres Especies de Oxígeno Reactivo Peróxido de Hidrógeno Arabidopsis thaliana Autophagy Singlet Oxygen Superoxide Radical Redox State |
| url | http://hdl.handle.net/20.500.12123/21950 https://www.sciencedirect.com/science/article/abs/pii/S0981942825003286 https://doi.org/10.1016/j.plaphy.2025.109800 |
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