Introgression of dwarfing allele dw1 reduced seed dormancy and increased pre-harvest sprouting susceptibility in grain sorghum converted lines
Grain sorghum (Sorghum bicolor L. moench) stands as a globally significant cereal crop but the adversity of pre-harvest sprouting (PHS) caused by reduced grain dormancy and moist conditions prior to harvest remains unsolved. Here, we identified a dormancy QTL using a Redlan×IS9530 RIL population, wh...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
Wiley
2025
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/21887 https://onlinelibrary.wiley.com/doi/10.1111/pbi.70007 https://doi.org/10.1111/pbi.70007 |
| _version_ | 1855486830551498752 |
|---|---|
| author | Rodríguez, María Verónica Sánchez, Diego Hernán Glison, Nicolás Ríos, Cristian Damián Demkura, Patricia Verónica Álvarez Correa, Cristian Camilo Fernandez, Luis German Filippi, Carla Valeria Heinz, Ruth Pardo, Pedro Rentería, Santiago Guillaumet, Lisandro Benech-Arnold, Roberto Luis |
| author_browse | Benech-Arnold, Roberto Luis Demkura, Patricia Verónica Fernandez, Luis German Filippi, Carla Valeria Glison, Nicolás Guillaumet, Lisandro Heinz, Ruth Pardo, Pedro Rentería, Santiago Rodríguez, María Verónica Ríos, Cristian Damián Sánchez, Diego Hernán Álvarez Correa, Cristian Camilo |
| author_facet | Rodríguez, María Verónica Sánchez, Diego Hernán Glison, Nicolás Ríos, Cristian Damián Demkura, Patricia Verónica Álvarez Correa, Cristian Camilo Fernandez, Luis German Filippi, Carla Valeria Heinz, Ruth Pardo, Pedro Rentería, Santiago Guillaumet, Lisandro Benech-Arnold, Roberto Luis |
| author_sort | Rodríguez, María Verónica |
| collection | INTA Digital |
| description | Grain sorghum (Sorghum bicolor L. moench) stands as a globally significant cereal crop but the adversity of pre-harvest sprouting (PHS) caused by reduced grain dormancy and moist conditions prior to harvest remains unsolved. Here, we identified a dormancy QTL using a Redlan×IS9530 RIL population, where parent lines are low in tannins and early flowering but otherwise contrasting in grain dormancy and plant height. We phenotyped this population in 2 years with informative PHS-related traits (grain germination index, embryo sensitivity to abscisic acid and in one year the actual natural sprouting), revealing a robust dormancy QTL in chromosome 9 (qDOR-9). This signal overlapped with associations found for plant height (caused by the dw1 locus, used for decades in sorghum improvement) and time to flowering. The effect of qDOR-9 was validated with independent near isogenic lines carrying the IS9530 “dormant” allele while maintaining the Redlan dw1 “short” allele. Additional analyses on Yellow Milo, from which the dw1 allele originated, implied that a low dormancy allele close to dw1 was introduced to Redlan—as well as to many other currently productive lines—by breeding efforts aimed at decreasing plant height, thus illustrating a new instance of genome erosion canalised by crop breeding. However, the introgression of qDOR-9 could enhance PHS tolerance in cultivated dw1-carrying backgrounds without affecting plant stature. |
| format | Artículo |
| id | INTA21887 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA218872025-04-03T14:57:12Z Introgression of dwarfing allele dw1 reduced seed dormancy and increased pre-harvest sprouting susceptibility in grain sorghum converted lines Rodríguez, María Verónica Sánchez, Diego Hernán Glison, Nicolás Ríos, Cristian Damián Demkura, Patricia Verónica Álvarez Correa, Cristian Camilo Fernandez, Luis German Filippi, Carla Valeria Heinz, Ruth Pardo, Pedro Rentería, Santiago Guillaumet, Lisandro Benech-Arnold, Roberto Luis Sorgo Granifero Brotación Fitomejoramiento Semilla Grain Sorghum Sprouting Plant Breeding Seed Seed Dormancy Dormancia de Semillas Pre-harvest Sprouting Seed Dormancy Dwarf-1 QTL mapping Grain sorghum (Sorghum bicolor L. moench) stands as a globally significant cereal crop but the adversity of pre-harvest sprouting (PHS) caused by reduced grain dormancy and moist conditions prior to harvest remains unsolved. Here, we identified a dormancy QTL using a Redlan×IS9530 RIL population, where parent lines are low in tannins and early flowering but otherwise contrasting in grain dormancy and plant height. We phenotyped this population in 2 years with informative PHS-related traits (grain germination index, embryo sensitivity to abscisic acid and in one year the actual natural sprouting), revealing a robust dormancy QTL in chromosome 9 (qDOR-9). This signal overlapped with associations found for plant height (caused by the dw1 locus, used for decades in sorghum improvement) and time to flowering. The effect of qDOR-9 was validated with independent near isogenic lines carrying the IS9530 “dormant” allele while maintaining the Redlan dw1 “short” allele. Additional analyses on Yellow Milo, from which the dw1 allele originated, implied that a low dormancy allele close to dw1 was introduced to Redlan—as well as to many other currently productive lines—by breeding efforts aimed at decreasing plant height, thus illustrating a new instance of genome erosion canalised by crop breeding. However, the introgression of qDOR-9 could enhance PHS tolerance in cultivated dw1-carrying backgrounds without affecting plant stature. EEA Pergamino Fil: Rodríguez, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina. Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina. Fil: Rodríguez, María Verónica. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Fisiología Vegetal; Argentina Fil: Sánchez, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina. Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina. Fil: Sánchez, Diego Hernán. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Fisiología Vegetal; Argentina Fil: Glison, Nicolás. Universidad de la República. Facultad de Agronomía. Departamento de Biología Vegetal. Laboratorio de Fisiología Vegetal; Uruguay Fil: Ríos, Cristian Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina. Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina. Fil: Ríos, Cristian Damián. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Cultivos Industriales; Argentina Fil: Demkura, Patricia Verónica. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura - Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Álvarez Correa, Cristian Camilo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina. Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina. Fil: Álvarez Correa, Cristian Camilo. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Cultivos Industriales; Argentina Fil: Fernández, Luis Germán. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Junín; Argentina Fil: Fernández, Luis Germán. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Filippi, Carla Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Heinz, Ruth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pardo, Pedro. Advanta Semillas SACI. Estación Experimental Venado Tuerto ; Argentina Fil: Rentería, Santiago. Advanta Semillas SACI. Estación Experimental Venado Tuerto ; Argentina Fil: Guillaumet, Lisandro. Advanta Semillas SACI. Estación Experimental Venado Tuerto; Argentina Fil: Benech-Arnold, Roberto Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina. Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA); Argentina. Fil: Benech-Arnold, Roberto Luis. Universidad de Buenos Aires. Facultad de Agronomía Departamento de Producción Vegetal. Cátedra de Cultivos Industriales; Argentina 2025-04-03T14:43:55Z 2025-04-03T14:43:55Z 2025-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21887 https://onlinelibrary.wiley.com/doi/10.1111/pbi.70007 1467-7644 1467-7652 (online) https://doi.org/10.1111/pbi.70007 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Plant Biotechnology Journal 23 (4) : 1-15. (April 2025) |
| spellingShingle | Sorgo Granifero Brotación Fitomejoramiento Semilla Grain Sorghum Sprouting Plant Breeding Seed Seed Dormancy Dormancia de Semillas Pre-harvest Sprouting Seed Dormancy Dwarf-1 QTL mapping Rodríguez, María Verónica Sánchez, Diego Hernán Glison, Nicolás Ríos, Cristian Damián Demkura, Patricia Verónica Álvarez Correa, Cristian Camilo Fernandez, Luis German Filippi, Carla Valeria Heinz, Ruth Pardo, Pedro Rentería, Santiago Guillaumet, Lisandro Benech-Arnold, Roberto Luis Introgression of dwarfing allele dw1 reduced seed dormancy and increased pre-harvest sprouting susceptibility in grain sorghum converted lines |
| title | Introgression of dwarfing allele dw1 reduced seed dormancy and increased pre-harvest sprouting susceptibility in grain sorghum converted lines |
| title_full | Introgression of dwarfing allele dw1 reduced seed dormancy and increased pre-harvest sprouting susceptibility in grain sorghum converted lines |
| title_fullStr | Introgression of dwarfing allele dw1 reduced seed dormancy and increased pre-harvest sprouting susceptibility in grain sorghum converted lines |
| title_full_unstemmed | Introgression of dwarfing allele dw1 reduced seed dormancy and increased pre-harvest sprouting susceptibility in grain sorghum converted lines |
| title_short | Introgression of dwarfing allele dw1 reduced seed dormancy and increased pre-harvest sprouting susceptibility in grain sorghum converted lines |
| title_sort | introgression of dwarfing allele dw1 reduced seed dormancy and increased pre harvest sprouting susceptibility in grain sorghum converted lines |
| topic | Sorgo Granifero Brotación Fitomejoramiento Semilla Grain Sorghum Sprouting Plant Breeding Seed Seed Dormancy Dormancia de Semillas Pre-harvest Sprouting Seed Dormancy Dwarf-1 QTL mapping |
| url | http://hdl.handle.net/20.500.12123/21887 https://onlinelibrary.wiley.com/doi/10.1111/pbi.70007 https://doi.org/10.1111/pbi.70007 |
| work_keys_str_mv | AT rodriguezmariaveronica introgressionofdwarfingalleledw1reducedseeddormancyandincreasedpreharvestsproutingsusceptibilityingrainsorghumconvertedlines AT sanchezdiegohernan introgressionofdwarfingalleledw1reducedseeddormancyandincreasedpreharvestsproutingsusceptibilityingrainsorghumconvertedlines AT glisonnicolas introgressionofdwarfingalleledw1reducedseeddormancyandincreasedpreharvestsproutingsusceptibilityingrainsorghumconvertedlines AT rioscristiandamian introgressionofdwarfingalleledw1reducedseeddormancyandincreasedpreharvestsproutingsusceptibilityingrainsorghumconvertedlines AT demkurapatriciaveronica introgressionofdwarfingalleledw1reducedseeddormancyandincreasedpreharvestsproutingsusceptibilityingrainsorghumconvertedlines AT alvarezcorreacristiancamilo introgressionofdwarfingalleledw1reducedseeddormancyandincreasedpreharvestsproutingsusceptibilityingrainsorghumconvertedlines AT fernandezluisgerman introgressionofdwarfingalleledw1reducedseeddormancyandincreasedpreharvestsproutingsusceptibilityingrainsorghumconvertedlines AT filippicarlavaleria introgressionofdwarfingalleledw1reducedseeddormancyandincreasedpreharvestsproutingsusceptibilityingrainsorghumconvertedlines AT heinzruth introgressionofdwarfingalleledw1reducedseeddormancyandincreasedpreharvestsproutingsusceptibilityingrainsorghumconvertedlines AT pardopedro introgressionofdwarfingalleledw1reducedseeddormancyandincreasedpreharvestsproutingsusceptibilityingrainsorghumconvertedlines AT renteriasantiago introgressionofdwarfingalleledw1reducedseeddormancyandincreasedpreharvestsproutingsusceptibilityingrainsorghumconvertedlines AT guillaumetlisandro introgressionofdwarfingalleledw1reducedseeddormancyandincreasedpreharvestsproutingsusceptibilityingrainsorghumconvertedlines AT benecharnoldrobertoluis introgressionofdwarfingalleledw1reducedseeddormancyandincreasedpreharvestsproutingsusceptibilityingrainsorghumconvertedlines |