Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations
The South American fruit fly Anastrepha fraterculus (Wiedmann) has a vast range extending from northern Mexico, through Central America, to South America where it is an extremely polyphagous pest of wild and cultivated fruits. It is a complex of cryptic species currently composed of eight recognised...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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MDPI
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/21879 https://www.mdpi.com/2075-4450/15/10/748 https://doi.org/10.3390/insects15100748 |
| _version_ | 1855038477287030784 |
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| author | Gomulski, Ludvik Vera, María Teresa Lanzavecchia, Silvia Beatriz Piccinno, Riccardo Fiorenza, Giulia De Luca, Daniel Carrizo, Beatriz Noemi Bouvet, Juan Pedro Viana, Valeria Angelica Cárceres, Carlos Enkerlin, Walther Malacrida, Anna Gasperi, Giuliano |
| author_browse | Bouvet, Juan Pedro Carrizo, Beatriz Noemi Cárceres, Carlos De Luca, Daniel Enkerlin, Walther Fiorenza, Giulia Gasperi, Giuliano Gomulski, Ludvik Lanzavecchia, Silvia Beatriz Malacrida, Anna Piccinno, Riccardo Vera, María Teresa Viana, Valeria Angelica |
| author_facet | Gomulski, Ludvik Vera, María Teresa Lanzavecchia, Silvia Beatriz Piccinno, Riccardo Fiorenza, Giulia De Luca, Daniel Carrizo, Beatriz Noemi Bouvet, Juan Pedro Viana, Valeria Angelica Cárceres, Carlos Enkerlin, Walther Malacrida, Anna Gasperi, Giuliano |
| author_sort | Gomulski, Ludvik |
| collection | INTA Digital |
| description | The South American fruit fly Anastrepha fraterculus (Wiedmann) has a vast range extending from northern Mexico, through Central America, to South America where it is an extremely polyphagous pest of wild and cultivated fruits. It is a complex of cryptic species currently composed of eight recognised morphotypes: “Mexican”, “Venezuelan”, “Andean”, “Peruvian”, “Ecuadorian”, and the three Brazilian morphotypes “Brazilian-1”, “Brazilian-2”, and “Brazilian-3”. Molecular markers that can identify the member species of the complex are crucial for the implementation of effective pest control measures, such as the sterile insect technique. The object of this study was to evaluate the use of the internal transcribed spacer 2 (ITS2) sequence for discriminating several members of the A. fraterculus cryptic species complex (Mexican, Peruvian, and Brazilian-1) and a related species, Anastrepha schultzi Blanchard. The analysis highlighted significant genetic differentiation between the evaluated morphotypes, allowed their discrimination within the A. fraterculus cryptic species complex, and provided new insights into their genetic relationships. The ITS2 marker provides a basis for the development of taxonomic keys for the discrimination of the cryptic taxa within the A. fraterculus cryptic species complex. ITS2 also represents an important marker for the poorly studied species A. schultzi. |
| format | info:ar-repo/semantics/artículo |
| id | INTA21879 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA218792025-04-03T12:05:41Z Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations Gomulski, Ludvik Vera, María Teresa Lanzavecchia, Silvia Beatriz Piccinno, Riccardo Fiorenza, Giulia De Luca, Daniel Carrizo, Beatriz Noemi Bouvet, Juan Pedro Viana, Valeria Angelica Cárceres, Carlos Enkerlin, Walther Malacrida, Anna Gasperi, Giuliano Anastrepha fraterculus Marcadores Genéticos Variación Genética Genetic Markers Genetic Variation Anastrepha schultzi Morfotipos Morphotypes The South American fruit fly Anastrepha fraterculus (Wiedmann) has a vast range extending from northern Mexico, through Central America, to South America where it is an extremely polyphagous pest of wild and cultivated fruits. It is a complex of cryptic species currently composed of eight recognised morphotypes: “Mexican”, “Venezuelan”, “Andean”, “Peruvian”, “Ecuadorian”, and the three Brazilian morphotypes “Brazilian-1”, “Brazilian-2”, and “Brazilian-3”. Molecular markers that can identify the member species of the complex are crucial for the implementation of effective pest control measures, such as the sterile insect technique. The object of this study was to evaluate the use of the internal transcribed spacer 2 (ITS2) sequence for discriminating several members of the A. fraterculus cryptic species complex (Mexican, Peruvian, and Brazilian-1) and a related species, Anastrepha schultzi Blanchard. The analysis highlighted significant genetic differentiation between the evaluated morphotypes, allowed their discrimination within the A. fraterculus cryptic species complex, and provided new insights into their genetic relationships. The ITS2 marker provides a basis for the development of taxonomic keys for the discrimination of the cryptic taxa within the A. fraterculus cryptic species complex. ITS2 also represents an important marker for the poorly studied species A. schultzi. Instituto de Genética Fil: Gomulski, Ludvik. University of Pavia. Department of Biology and Biotechnology; Italia Fil: Vera, María Teresa. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina Fil: Piccinno, Riccardo. University of Pavia. Department of Biology and Biotechnology; Italia Fil: Fiorenza, Giulia. University of Pavia. Department of Biology and Biotechnology; Italia Fil: De Luca, Daniel. University of Pavia. Department of Biology and Biotechnology; Italia Fil: Carrizo, Beatriz Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Bouvet, Juan Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia. Grupo de Protección Vegetal; Argentina Fil: Viana, Valeria Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia. Grupo de Protección Vegetal; Argentina Fil: Cárceres, Carlos. Joint FAO/IAEA Centre. Insect Pest Control Section; Austria Fil: Enkerlin, Walther. Joint FAO/IAEA Centre. Insect Pest Control Section; Austria Fil: Malacrida, Anna. University of Pavia. Department of Biology and Biotechnology; Italia Fil: Gasperi, Giuliano. University of Pavia. Department of Biology and Biotechnology; Italia 2025-04-03T11:58:17Z 2025-04-03T11:58:17Z 2024-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21879 https://www.mdpi.com/2075-4450/15/10/748 2075-4450 https://doi.org/10.3390/insects15100748 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Insects 15 (10) : 748 (October 2024) |
| spellingShingle | Anastrepha fraterculus Marcadores Genéticos Variación Genética Genetic Markers Genetic Variation Anastrepha schultzi Morfotipos Morphotypes Gomulski, Ludvik Vera, María Teresa Lanzavecchia, Silvia Beatriz Piccinno, Riccardo Fiorenza, Giulia De Luca, Daniel Carrizo, Beatriz Noemi Bouvet, Juan Pedro Viana, Valeria Angelica Cárceres, Carlos Enkerlin, Walther Malacrida, Anna Gasperi, Giuliano Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations |
| title | Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations |
| title_full | Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations |
| title_fullStr | Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations |
| title_full_unstemmed | Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations |
| title_short | Molecular markers for analyses of genetic diversity within the Anastrepha fraterculus complex with emphasis on Argentine populations |
| title_sort | molecular markers for analyses of genetic diversity within the anastrepha fraterculus complex with emphasis on argentine populations |
| topic | Anastrepha fraterculus Marcadores Genéticos Variación Genética Genetic Markers Genetic Variation Anastrepha schultzi Morfotipos Morphotypes |
| url | http://hdl.handle.net/20.500.12123/21879 https://www.mdpi.com/2075-4450/15/10/748 https://doi.org/10.3390/insects15100748 |
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