Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH)
Background: The South American fruit fly Anastrepha fraterculus sp. 1 belongs to a cryptic species complex with only a single morphotype present in Argentina, which possess polymorphisms in the sex chromosomes, identified by two X (X1, X2) and two Y (Y5, Y6) variants. Our aim was to explore the mole...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer
2025
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/21878 https://link.springer.com/article/10.1007/s11033-025-10277-w https://doi.org/10.1007/s11033-025-10277-w |
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| author | Giardini, Maria Cecilia Milla, Fabian Horacio Conte, Claudia Alejandra Lanzavecchia, Silvia Beatriz Nieves, Mariela |
| author_browse | Conte, Claudia Alejandra Giardini, Maria Cecilia Lanzavecchia, Silvia Beatriz Milla, Fabian Horacio Nieves, Mariela |
| author_facet | Giardini, Maria Cecilia Milla, Fabian Horacio Conte, Claudia Alejandra Lanzavecchia, Silvia Beatriz Nieves, Mariela |
| author_sort | Giardini, Maria Cecilia |
| collection | INTA Digital |
| description | Background: The South American fruit fly Anastrepha fraterculus sp. 1 belongs to a cryptic species complex with only a single morphotype present in Argentina, which possess polymorphisms in the sex chromosomes, identified by two X (X1, X2) and two Y (Y5, Y6) variants. Our aim was to explore the molecular composition of the sex chromosomes identified in the laboratory strain Af-Y-short due to it possesses the most frequent sex chromosomes variants, and the smallest Y chromosome found in wild populations and laboratory colonies of A. fraterculus sp. 1.
Methods and results: Whole Comparative Genomic Hybridization (W-CGH) was applied to the Af-Y-short laboratory strain to identify potential differences in the size and composition of highly repetitive DNA blocks between individuals, morphs or sexes. Our results showed that the X1 and X2 share complete homology in euchromatic regions, also sharing similar sequences with the Y5 chromosome in the centromeric region. Females X1X1 and X1X2 exhibited repetitive DNA in telomeric regions which contributed to their morphological differentiation, while the Y chromosome evidenced the accumulation of specific and differential repetitive sequences. In contrast, autosomes showed balanced signal with no sex-chromosome-specific sequence accumulation.
Conclusions: Our findings highlight the role of repetitive DNA in the differentiation and evolution of sex chromosomes in A. fraterculus sp. 1, shedding new light on the origin and diversification of Anastrepha species in South America and providing valuable cytogenetic insights for future research. |
| format | info:ar-repo/semantics/artículo |
| id | INTA21878 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA218782025-04-03T11:49:37Z Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH) Giardini, Maria Cecilia Milla, Fabian Horacio Conte, Claudia Alejandra Lanzavecchia, Silvia Beatriz Nieves, Mariela Sex Chromosomes Hybridization Cromosomas Sexuales Anastrepha fraterculus Hibridación Molecular Differentiation Diferenciación Molecular Background: The South American fruit fly Anastrepha fraterculus sp. 1 belongs to a cryptic species complex with only a single morphotype present in Argentina, which possess polymorphisms in the sex chromosomes, identified by two X (X1, X2) and two Y (Y5, Y6) variants. Our aim was to explore the molecular composition of the sex chromosomes identified in the laboratory strain Af-Y-short due to it possesses the most frequent sex chromosomes variants, and the smallest Y chromosome found in wild populations and laboratory colonies of A. fraterculus sp. 1. Methods and results: Whole Comparative Genomic Hybridization (W-CGH) was applied to the Af-Y-short laboratory strain to identify potential differences in the size and composition of highly repetitive DNA blocks between individuals, morphs or sexes. Our results showed that the X1 and X2 share complete homology in euchromatic regions, also sharing similar sequences with the Y5 chromosome in the centromeric region. Females X1X1 and X1X2 exhibited repetitive DNA in telomeric regions which contributed to their morphological differentiation, while the Y chromosome evidenced the accumulation of specific and differential repetitive sequences. In contrast, autosomes showed balanced signal with no sex-chromosome-specific sequence accumulation. Conclusions: Our findings highlight the role of repetitive DNA in the differentiation and evolution of sex chromosomes in A. fraterculus sp. 1, shedding new light on the origin and diversification of Anastrepha species in South America and providing valuable cytogenetic insights for future research. Instituto de Genética Fil: Giardini, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina Fil: Giardini, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Giardini, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina Fil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Milla, Fabian Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Conte, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nieves, Mariela. Dirección de investigaciones Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno” (CEMIC). Grupo de estudios en Arquitectura Genómica de Mamíferos (arGENma); Argentina Fil: Nieves, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2025-04-03T11:40:16Z 2025-04-03T11:40:16Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21878 https://link.springer.com/article/10.1007/s11033-025-10277-w 1573-4978 https://doi.org/10.1007/s11033-025-10277-w eng info:eu-repograntAgreement/INTA/2023-PD-L01-I106, Plagas y enfermedades emergentes, cuarentenarias y limitantes en cultivos frutícolas: estrategias para mejorar su prevención, detección precoz y control info:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada. info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Molecular Biology Reports 52 (1) : 250 (Published: 20 February 2025) |
| spellingShingle | Sex Chromosomes Hybridization Cromosomas Sexuales Anastrepha fraterculus Hibridación Molecular Differentiation Diferenciación Molecular Giardini, Maria Cecilia Milla, Fabian Horacio Conte, Claudia Alejandra Lanzavecchia, Silvia Beatriz Nieves, Mariela Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH) |
| title | Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH) |
| title_full | Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH) |
| title_fullStr | Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH) |
| title_full_unstemmed | Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH) |
| title_short | Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH) |
| title_sort | exploring the molecular differentiation of sex chromosomes in anastrepha fraterculus sp 1 using comparative genomic hybridization cgh |
| topic | Sex Chromosomes Hybridization Cromosomas Sexuales Anastrepha fraterculus Hibridación Molecular Differentiation Diferenciación Molecular |
| url | http://hdl.handle.net/20.500.12123/21878 https://link.springer.com/article/10.1007/s11033-025-10277-w https://doi.org/10.1007/s11033-025-10277-w |
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