Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes
Losses produced by virus diseases depend mostly on symptom severity. Turnip mosaic virus (TuMV) is one of the most damaging and widespread potyvirus infecting members of the family Brassicaceae, including Arabidopsis thaliana. We used JPN1 and UK1 TuMV strains to characterize viral infections regard...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/2184 https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-07-13-0190-R http://dx.doi.org/10.1094/MPMI-07-13-0190-R |
| _version_ | 1855483009072889856 |
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| author | Manacorda, Carlos Augusto Mansilla, Carmen Debat, Humberto Julio Zavallo, Diego Sánchez, Flora Ponz, Fernando Asurmendi, Sebastian |
| author_browse | Asurmendi, Sebastian Debat, Humberto Julio Manacorda, Carlos Augusto Mansilla, Carmen Ponz, Fernando Sánchez, Flora Zavallo, Diego |
| author_facet | Manacorda, Carlos Augusto Mansilla, Carmen Debat, Humberto Julio Zavallo, Diego Sánchez, Flora Ponz, Fernando Asurmendi, Sebastian |
| author_sort | Manacorda, Carlos Augusto |
| collection | INTA Digital |
| description | Losses produced by virus diseases depend mostly on symptom severity. Turnip mosaic virus (TuMV) is one of the most damaging and widespread potyvirus infecting members of the family Brassicaceae, including Arabidopsis thaliana. We used JPN1 and UK1 TuMV strains to characterize viral infections regarding symptom development, senescence progression, antioxidant response, reactive oxygen species (ROS) accumulation, and transcriptional profiling. Both isolates, despite accumulating similar viral titers, induced different symptomatology and strong differences in oxidative status. Early differences in several senescenceassociated
genes linked to the ORE1 and ORS1 regulatory networks as well as persistent divergence in key ROS production and scavenging systems of the plant were detected. However, at a later stage, both strains induced nutrient competition, indicating that senescence rates are influenced by different mechanisms upon viral infections. Analyses of ORE1 and ORS1 levels in infected Brassica juncea plants showed a similar pattern, suggesting a conserved differential response to both strains in Brassicaceae spp. Transcriptional analysis of the ORE1 and ORS1 regulons showed similarities between salicylic acid (SA) response and the early induction triggered by UK1, the most severe strain. By means of SA-defective NahG transgenic plants, we found that differential senescence progression and ROS accumulation between strains rely on an intact SA pathway. |
| format | Artículo |
| id | INTA2184 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA21842018-04-05T18:21:21Z Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes Manacorda, Carlos Augusto Mansilla, Carmen Debat, Humberto Julio Zavallo, Diego Sánchez, Flora Ponz, Fernando Asurmendi, Sebastian Acido Salicílico Envejecimiento Nabo Brassica Juncea Virus de las Plantas Plant Viruses Turnips Aging Salicylic Acids Virus del Mosaico de Nabo Losses produced by virus diseases depend mostly on symptom severity. Turnip mosaic virus (TuMV) is one of the most damaging and widespread potyvirus infecting members of the family Brassicaceae, including Arabidopsis thaliana. We used JPN1 and UK1 TuMV strains to characterize viral infections regarding symptom development, senescence progression, antioxidant response, reactive oxygen species (ROS) accumulation, and transcriptional profiling. Both isolates, despite accumulating similar viral titers, induced different symptomatology and strong differences in oxidative status. Early differences in several senescenceassociated genes linked to the ORE1 and ORS1 regulatory networks as well as persistent divergence in key ROS production and scavenging systems of the plant were detected. However, at a later stage, both strains induced nutrient competition, indicating that senescence rates are influenced by different mechanisms upon viral infections. Analyses of ORE1 and ORS1 levels in infected Brassica juncea plants showed a similar pattern, suggesting a conserved differential response to both strains in Brassicaceae spp. Transcriptional analysis of the ORE1 and ORS1 regulons showed similarities between salicylic acid (SA) response and the early induction triggered by UK1, the most severe strain. By means of SA-defective NahG transgenic plants, we found that differential senescence progression and ROS accumulation between strains rely on an intact SA pathway. Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Mansilla, Carmen. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas; España Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sánchez, Flora. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas; España Fil: Ponz, Fernando. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas; España Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-04-05T18:11:25Z 2018-04-05T18:11:25Z 2013-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2184 https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-07-13-0190-R 0894-0282 1943-7706 (online version) http://dx.doi.org/10.1094/MPMI-07-13-0190-R eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Molecular plant-microbe interactions 26 (12) : 1486-1498. (2013) |
| spellingShingle | Acido Salicílico Envejecimiento Nabo Brassica Juncea Virus de las Plantas Plant Viruses Turnips Aging Salicylic Acids Virus del Mosaico de Nabo Manacorda, Carlos Augusto Mansilla, Carmen Debat, Humberto Julio Zavallo, Diego Sánchez, Flora Ponz, Fernando Asurmendi, Sebastian Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes |
| title | Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes |
| title_full | Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes |
| title_fullStr | Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes |
| title_full_unstemmed | Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes |
| title_short | Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes |
| title_sort | salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes |
| topic | Acido Salicílico Envejecimiento Nabo Brassica Juncea Virus de las Plantas Plant Viruses Turnips Aging Salicylic Acids Virus del Mosaico de Nabo |
| url | http://hdl.handle.net/20.500.12123/2184 https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-07-13-0190-R http://dx.doi.org/10.1094/MPMI-07-13-0190-R |
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