Botanical-Based Strategies for Controlling Xanthomonas spp. in Cotton and Citrus: In Vitro and In Vivo Evaluation
Citrus canker, caused by Xanthomonas citri subsp. citri, and bacterial blight, caused by Xanthomonas citri subsp. malvacearum, results in substantial economic losses worldwide, and searching for new antibacterial agents is a critical challenge. In this study, regional isolates AE28 and RQ3 were obta...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/21772 https://www.mdpi.com/2223-7747/14/6/957 https://doi.org/10.3390/plants14060957 |
| _version_ | 1855486817871069184 |
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| author | Roeschlin, Roxana Andrea Favaro, María Alejandra Bertinat, Bruno Lorenzini, Fernando Paytas, Marcelo Javier Fernandez, Laura Noemí Marano, María Rosa Derita, Marcos Gabriel |
| author_browse | Bertinat, Bruno Derita, Marcos Gabriel Favaro, María Alejandra Fernandez, Laura Noemí Lorenzini, Fernando Marano, María Rosa Paytas, Marcelo Javier Roeschlin, Roxana Andrea |
| author_facet | Roeschlin, Roxana Andrea Favaro, María Alejandra Bertinat, Bruno Lorenzini, Fernando Paytas, Marcelo Javier Fernandez, Laura Noemí Marano, María Rosa Derita, Marcos Gabriel |
| author_sort | Roeschlin, Roxana Andrea |
| collection | INTA Digital |
| description | Citrus canker, caused by Xanthomonas citri subsp. citri, and bacterial blight, caused by Xanthomonas citri subsp. malvacearum, results in substantial economic losses worldwide, and searching for new antibacterial agents is a critical challenge. In this study, regional isolates AE28 and RQ3 were obtained from characteristic lesions on Citrus limon and Gossypium hirsutum, respectively. Essential oils extracted by steam distillation from the fresh aerial parts of Pelargonium graveolens and Schinus molle exhibited complete (100%) inhibition of bacterial growth in vitro at a concentration of 1000 ppm, as determined by diffusion tests. To evaluate the potential of these essential oils for controlling Xanthomonas-induced diseases, in vivo assays were conducted on lemon leaves and cotton cotyledons inoculated with the regional AE28 and RQ3 strains. Two treatment approaches were tested: preventive application (24 h before inoculation) and curative application (24 h after inoculation). Preventive and curative treatments with P. graveolens essential oil significantly reduced citrus canker severity, whereas S. molle essential oil did not show a significant reduction compared to the control. In contrast, regardless of the treatment’s timing, both essential oils effectively reduced bacterial blight severity in cotton cotyledons by approximately 1.5-fold. Gas chromatography–mass spectrometry (GC-MS) analysis identified geraniol and citronellol as the major components of P. graveolens essential oil, while limonene and t-cadinol were predominant in S. molle. These findings highlight the promising potential of botanical products as bactericidal agents, warranting further research to optimize their application and efficacy. |
| format | Artículo |
| id | INTA21772 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA217722025-03-21T10:46:14Z Botanical-Based Strategies for Controlling Xanthomonas spp. in Cotton and Citrus: In Vitro and In Vivo Evaluation Roeschlin, Roxana Andrea Favaro, María Alejandra Bertinat, Bruno Lorenzini, Fernando Paytas, Marcelo Javier Fernandez, Laura Noemí Marano, María Rosa Derita, Marcos Gabriel Xanthomonas Cotton Gossypium hirsutum Citrus limon Lemons Diseases Control Antibacterial Plants Schinus molle Pelargonium graveolens Persicaria Algodón Limón Acido Control de Enfermedades Plantas Antibacterianas Xanthomonas citri subsp. citri Citrus canker, caused by Xanthomonas citri subsp. citri, and bacterial blight, caused by Xanthomonas citri subsp. malvacearum, results in substantial economic losses worldwide, and searching for new antibacterial agents is a critical challenge. In this study, regional isolates AE28 and RQ3 were obtained from characteristic lesions on Citrus limon and Gossypium hirsutum, respectively. Essential oils extracted by steam distillation from the fresh aerial parts of Pelargonium graveolens and Schinus molle exhibited complete (100%) inhibition of bacterial growth in vitro at a concentration of 1000 ppm, as determined by diffusion tests. To evaluate the potential of these essential oils for controlling Xanthomonas-induced diseases, in vivo assays were conducted on lemon leaves and cotton cotyledons inoculated with the regional AE28 and RQ3 strains. Two treatment approaches were tested: preventive application (24 h before inoculation) and curative application (24 h after inoculation). Preventive and curative treatments with P. graveolens essential oil significantly reduced citrus canker severity, whereas S. molle essential oil did not show a significant reduction compared to the control. In contrast, regardless of the treatment’s timing, both essential oils effectively reduced bacterial blight severity in cotton cotyledons by approximately 1.5-fold. Gas chromatography–mass spectrometry (GC-MS) analysis identified geraniol and citronellol as the major components of P. graveolens essential oil, while limonene and t-cadinol were predominant in S. molle. These findings highlight the promising potential of botanical products as bactericidal agents, warranting further research to optimize their application and efficacy. EEA Reconquista Fil: Roeschlin, Roxana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; Argentina Fil: Roeschlin, Roxana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Favaro, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral; Argentina Fil: Favaro, María Alejandra. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Instituto de Ciencias Agropecuarias del Litoral; Argentina Fil: Favaro, María Alejandra. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina Fil: Bertinat, Bruno. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina Fil: Lorenzini, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; Argentina Fil: Lorenzini, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Paytas, Marcelo Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; Argentina Fil: Fernandez, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral; Argentina. Fil: Fernandez, Laura Noemí. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Instituto de Ciencias Agropecuarias del Litoral; Argentina Fil: Fernandez, Laura Noemí. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias; Argentina Fil: Marano, María Rosa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR); Argentina Fil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (IBR); Argentina Fil: Derita, Marcos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral; Argentina. Fil: Derita, Marcos Gabriel. Universidad Nacional del Litoral. Facultad de Ciencias Agrarias. Instituto de Ciencias Agropecuarias del Litoral; Argentina Fil: Derita, Marcos Gabriel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Farmacognosia; Argentina 2025-03-21T10:42:26Z 2025-03-21T10:42:26Z 2025-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21772 https://www.mdpi.com/2223-7747/14/6/957 2223-7747 https://doi.org/10.3390/plants14060957 eng info:eu-repograntAgreement/INTA/2023-PE-L01-I070, Aportes en el diseño y aplicación de tecnologías para el incremento de la productividad y sostenibilidad de la cadena algodonera info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Plants 14 (6) : 957. (March 2025) |
| spellingShingle | Xanthomonas Cotton Gossypium hirsutum Citrus limon Lemons Diseases Control Antibacterial Plants Schinus molle Pelargonium graveolens Persicaria Algodón Limón Acido Control de Enfermedades Plantas Antibacterianas Xanthomonas citri subsp. citri Roeschlin, Roxana Andrea Favaro, María Alejandra Bertinat, Bruno Lorenzini, Fernando Paytas, Marcelo Javier Fernandez, Laura Noemí Marano, María Rosa Derita, Marcos Gabriel Botanical-Based Strategies for Controlling Xanthomonas spp. in Cotton and Citrus: In Vitro and In Vivo Evaluation |
| title | Botanical-Based Strategies for Controlling Xanthomonas spp. in Cotton and Citrus: In Vitro and In Vivo Evaluation |
| title_full | Botanical-Based Strategies for Controlling Xanthomonas spp. in Cotton and Citrus: In Vitro and In Vivo Evaluation |
| title_fullStr | Botanical-Based Strategies for Controlling Xanthomonas spp. in Cotton and Citrus: In Vitro and In Vivo Evaluation |
| title_full_unstemmed | Botanical-Based Strategies for Controlling Xanthomonas spp. in Cotton and Citrus: In Vitro and In Vivo Evaluation |
| title_short | Botanical-Based Strategies for Controlling Xanthomonas spp. in Cotton and Citrus: In Vitro and In Vivo Evaluation |
| title_sort | botanical based strategies for controlling xanthomonas spp in cotton and citrus in vitro and in vivo evaluation |
| topic | Xanthomonas Cotton Gossypium hirsutum Citrus limon Lemons Diseases Control Antibacterial Plants Schinus molle Pelargonium graveolens Persicaria Algodón Limón Acido Control de Enfermedades Plantas Antibacterianas Xanthomonas citri subsp. citri |
| url | http://hdl.handle.net/20.500.12123/21772 https://www.mdpi.com/2223-7747/14/6/957 https://doi.org/10.3390/plants14060957 |
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