Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina
Plant viruses that are members of the Geminiviridae family have circular single-stranded DNA (ssDNA) genome and are responsible for major crop diseases worldwide. We have identified and characterized a novel monopartite geminivirus infecting tomato in Argentina. The full-length genome was cloned and...
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2018
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2017.02665/full http://hdl.handle.net/20.500.12123/2169 https://doi.org/10.3389/fmicb.2017.02665 |
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| author | Vaghi Medina, Carlos Gaston Teppa, Elin Bornancini, Veronica Andrea Flores, Ceferino Rene Marino Buslje, Cristina Lopez Lambertini, Paola Maria |
| author_browse | Bornancini, Veronica Andrea Flores, Ceferino Rene Lopez Lambertini, Paola Maria Marino Buslje, Cristina Teppa, Elin Vaghi Medina, Carlos Gaston |
| author_facet | Vaghi Medina, Carlos Gaston Teppa, Elin Bornancini, Veronica Andrea Flores, Ceferino Rene Marino Buslje, Cristina Lopez Lambertini, Paola Maria |
| author_sort | Vaghi Medina, Carlos Gaston |
| collection | INTA Digital |
| description | Plant viruses that are members of the Geminiviridae family have circular single-stranded DNA (ssDNA) genome and are responsible for major crop diseases worldwide. We have identified and characterized a novel monopartite geminivirus infecting tomato in Argentina. The full-length genome was cloned and sequenced. The genome-wide pairwise identity calculation that resulted in a maximum of 63% identity with all of other known geminiviruses indicated that it is a new geminivirus species. Biolistic infected plants presented interveinal yellowing, apical leaf curling and extreme root hypotrophy. Thus, the name proposed for this species is tomato apical leaf curl virus (ToALCV). The phylogenetic inferences suggested different evolutionary relationships for the replication-associated protein (Rep) and the coat protein (CP). Besides, the sequence similarity network (SSN) protein analyses showed that the complementary-sense gene products (RepA, Rep and C3) are similar to capulavirus while the viron-sense gene products (CP, MP and V3) are similar to topocuvirus, curtovirus and becurtovirus. Based on the data presented, ToALCV genome appears to have "modular organization" supported by its recombination origin. Analyses of the specificity-determining positions (SDPs) of the CP of geminiviruses defined nine subgroups that include geminiviruses that share the same type of insect vector. Our sequences were clustered with the sequences of topocuvirus, whose vector is the treehopper, Micrutalis malleifera. Also, a set of the highest scored amino acid residues was predicted for the CP, which could determine differences in virus transmission specificity. We predict that a treehopper could be the vector of ToALCV, but transmission assays need to be performed to confirm this. Given everything we demonstrate in this paper, ToALCV can be considered a type member of a new putative genus of the Geminiviridae family. |
| format | Artículo |
| id | INTA2169 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA21692018-04-04T18:35:51Z Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina Vaghi Medina, Carlos Gaston Teppa, Elin Bornancini, Veronica Andrea Flores, Ceferino Rene Marino Buslje, Cristina Lopez Lambertini, Paola Maria Solanum lycopersicum Tomate Coat Proteins Recombination Tomato Sequence Similarity Network Treehopper Plant viruses that are members of the Geminiviridae family have circular single-stranded DNA (ssDNA) genome and are responsible for major crop diseases worldwide. We have identified and characterized a novel monopartite geminivirus infecting tomato in Argentina. The full-length genome was cloned and sequenced. The genome-wide pairwise identity calculation that resulted in a maximum of 63% identity with all of other known geminiviruses indicated that it is a new geminivirus species. Biolistic infected plants presented interveinal yellowing, apical leaf curling and extreme root hypotrophy. Thus, the name proposed for this species is tomato apical leaf curl virus (ToALCV). The phylogenetic inferences suggested different evolutionary relationships for the replication-associated protein (Rep) and the coat protein (CP). Besides, the sequence similarity network (SSN) protein analyses showed that the complementary-sense gene products (RepA, Rep and C3) are similar to capulavirus while the viron-sense gene products (CP, MP and V3) are similar to topocuvirus, curtovirus and becurtovirus. Based on the data presented, ToALCV genome appears to have "modular organization" supported by its recombination origin. Analyses of the specificity-determining positions (SDPs) of the CP of geminiviruses defined nine subgroups that include geminiviruses that share the same type of insect vector. Our sequences were clustered with the sequences of topocuvirus, whose vector is the treehopper, Micrutalis malleifera. Also, a set of the highest scored amino acid residues was predicted for the CP, which could determine differences in virus transmission specificity. We predict that a treehopper could be the vector of ToALCV, but transmission assays need to be performed to confirm this. Given everything we demonstrate in this paper, ToALCV can be considered a type member of a new putative genus of the Geminiviridae family. INTA. CIAP. Instituto de Patología Vegetal Fil: Vaghi Medina, Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil:Teppa, Elin. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir; Argentina Fil: Bornancini, Verónica A. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Flores, Ceferino R. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentina Fil: Marino Buslje, Cristina. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir; Argentina Fil: López Lambertini, Paola M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2018-04-04T18:24:45Z 2018-04-04T18:24:45Z 2018-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.frontiersin.org/articles/10.3389/fmicb.2017.02665/full http://hdl.handle.net/20.500.12123/2169 1664-302X https://doi.org/10.3389/fmicb.2017.02665 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina (nation) Frontiers in Microbiology January 2018 |
| spellingShingle | Solanum lycopersicum Tomate Coat Proteins Recombination Tomato Sequence Similarity Network Treehopper Vaghi Medina, Carlos Gaston Teppa, Elin Bornancini, Veronica Andrea Flores, Ceferino Rene Marino Buslje, Cristina Lopez Lambertini, Paola Maria Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina |
| title | Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina |
| title_full | Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina |
| title_fullStr | Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina |
| title_full_unstemmed | Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina |
| title_short | Tomato apical leaf curl virus: a novel, monopartite geminivirus detected in tomatoes in Argentina |
| title_sort | tomato apical leaf curl virus a novel monopartite geminivirus detected in tomatoes in argentina |
| topic | Solanum lycopersicum Tomate Coat Proteins Recombination Tomato Sequence Similarity Network Treehopper |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2017.02665/full http://hdl.handle.net/20.500.12123/2169 https://doi.org/10.3389/fmicb.2017.02665 |
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