Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression
Sweet orange (Citrus sinensis), one of the most important fruit crops worldwide, may suffer from disease symptoms induced by virus infections, thus resulting in dramatic economic losses. Here, we show that the infection of sweet orange plants with two isolates of Citrus psorosis virus (CPsV) expre...
| Main Authors: | , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/2166 https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12282 |
| _version_ | 1855483006442012672 |
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| author | Reyes, Carina Andrea Ocolotobiche, Eliana Evelina Marmisollé, Facundo E. Robles Luna, Gabriel Bazzini, Ariel Alejandro Asurmendi, Sebastian García, María Laura |
| author_browse | Asurmendi, Sebastian Bazzini, Ariel Alejandro García, María Laura Marmisollé, Facundo E. Ocolotobiche, Eliana Evelina Reyes, Carina Andrea Robles Luna, Gabriel |
| author_facet | Reyes, Carina Andrea Ocolotobiche, Eliana Evelina Marmisollé, Facundo E. Robles Luna, Gabriel Bazzini, Ariel Alejandro Asurmendi, Sebastian García, María Laura |
| author_sort | Reyes, Carina Andrea |
| collection | INTA Digital |
| description | Sweet orange (Citrus sinensis), one of the most important fruit crops worldwide, may suffer from disease symptoms induced by
virus infections, thus resulting in dramatic economic losses. Here, we show that the infection of sweet orange plants with two
isolates of Citrus psorosis virus (CPsV) expressing different symptomatology alters the accumulation of a set of endogenous
microRNAs (miRNAs). Within these miRNAs, miR156, miR167 and miR171 were the most down-regulated, with almost a three-fold reduction in infected samples. This down-regulation led to a concomitant up-regulation of some of their targets, such as Squamosa promoter-binding protein-like 9 and 13, as well as Scarecrow-like 6. The processing of miRNA precursors, pre-miR156 and premiR171, in sweet orange seems to be affected by the virus. For instance, virus infection increases the level of unprocessed precursors, which is accompanied by a concomitant decrease in mature species accumulation. miR156a primary transcript accumulation remained unaltered, thus strongly suggesting a processing deregulation for this transcript. The co-immunoprecipitation of viral 24K protein with pre-miR156a or pre-miR171a suggests that the alteration in the processing of these precursors might be caused by a direct or indirect interaction with this particular viral protein. This result is also consistent with the nuclear localization of both miRNA precursors and the CPsV 24K protein. This study contributes to the understanding of the manner in which a virus can alter host regulatory mechanisms, particularly miRNA biogenesis and target expression. |
| format | Artículo |
| id | INTA2166 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA21662018-06-28T17:03:47Z Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression Reyes, Carina Andrea Ocolotobiche, Eliana Evelina Marmisollé, Facundo E. Robles Luna, Gabriel Bazzini, Ariel Alejandro Asurmendi, Sebastian García, María Laura Virus de las Plantas Citrus ARN Naranja Dulce Sweet Oranges RNA Plant Viruses Virus de la Psoriasis de los Cítricos Ophiovirus Sweet orange (Citrus sinensis), one of the most important fruit crops worldwide, may suffer from disease symptoms induced by virus infections, thus resulting in dramatic economic losses. Here, we show that the infection of sweet orange plants with two isolates of Citrus psorosis virus (CPsV) expressing different symptomatology alters the accumulation of a set of endogenous microRNAs (miRNAs). Within these miRNAs, miR156, miR167 and miR171 were the most down-regulated, with almost a three-fold reduction in infected samples. This down-regulation led to a concomitant up-regulation of some of their targets, such as Squamosa promoter-binding protein-like 9 and 13, as well as Scarecrow-like 6. The processing of miRNA precursors, pre-miR156 and premiR171, in sweet orange seems to be affected by the virus. For instance, virus infection increases the level of unprocessed precursors, which is accompanied by a concomitant decrease in mature species accumulation. miR156a primary transcript accumulation remained unaltered, thus strongly suggesting a processing deregulation for this transcript. The co-immunoprecipitation of viral 24K protein with pre-miR156a or pre-miR171a suggests that the alteration in the processing of these precursors might be caused by a direct or indirect interaction with this particular viral protein. This result is also consistent with the nuclear localization of both miRNA precursors and the CPsV 24K protein. This study contributes to the understanding of the manner in which a virus can alter host regulatory mechanisms, particularly miRNA biogenesis and target expression. Fil: Reyes, Carina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Ocolotobiche, Eliana Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Marmisollé, Facundo E. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Borniego, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Bazzini, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: García, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina 2018-04-04T15:35:49Z 2018-04-04T15:35:49Z 2016-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2166 https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12282 1464-6722 eng info:eu-repo/semantics/restrictedAccess application/pdf Molecular plant pathology 17 (3) : 317-329. (2016 Apr.) |
| spellingShingle | Virus de las Plantas Citrus ARN Naranja Dulce Sweet Oranges RNA Plant Viruses Virus de la Psoriasis de los Cítricos Ophiovirus Reyes, Carina Andrea Ocolotobiche, Eliana Evelina Marmisollé, Facundo E. Robles Luna, Gabriel Bazzini, Ariel Alejandro Asurmendi, Sebastian García, María Laura Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression |
| title | Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression |
| title_full | Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression |
| title_fullStr | Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression |
| title_full_unstemmed | Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression |
| title_short | Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression |
| title_sort | citrus psorosis virus 24k protein interacts with citrus mirna precursors affects their processing and subsequent mirna accumulation and target expression |
| topic | Virus de las Plantas Citrus ARN Naranja Dulce Sweet Oranges RNA Plant Viruses Virus de la Psoriasis de los Cítricos Ophiovirus |
| url | http://hdl.handle.net/20.500.12123/2166 https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12282 |
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