Failure of doramectin and ivermectin in preventing Cochliomyia hominivorax myiasis in a subtropical region: A pharmacokinetic-pharmacodynamic study
The aim of this work is to present a case study where the failure of IVM 3.15 % and DRM 1 % to prevent natural infestations of C. hominivorax larvae in Argentina is investigated based on field efficacy tests and a pharmacokinetic and pharmacodynamic analysis. Thirty male crossbred Braford calves wer...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2025
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/21628 https://www.sciencedirect.com/science/article/abs/pii/S0304401724002735 https://doi.org/10.1016/j.vetpar.2024.110384 |
| _version_ | 1855486793955147776 |
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| author | Muchiut, Sebastian Miró, Maria Victoria Anziani, Oscar Sergio Nava, Santiago Lifschitz, Adrian Luis |
| author_browse | Anziani, Oscar Sergio Lifschitz, Adrian Luis Miró, Maria Victoria Muchiut, Sebastian Nava, Santiago |
| author_facet | Muchiut, Sebastian Miró, Maria Victoria Anziani, Oscar Sergio Nava, Santiago Lifschitz, Adrian Luis |
| author_sort | Muchiut, Sebastian |
| collection | INTA Digital |
| description | The aim of this work is to present a case study where the failure of IVM 3.15 % and DRM 1 % to prevent natural infestations of C. hominivorax larvae in Argentina is investigated based on field efficacy tests and a pharmacokinetic and pharmacodynamic analysis. Thirty male crossbred Braford calves were randomly assigned to three experimental groups (n = 10), the IVM 3.15 % group (subcutaneously at 630 µg/kg), the DRM 1 % group (subcutaneously at 200 µg/kg) and the control group (saline solution subcutaneously). All treatments were performed at the time of castration surgery through a scrotal incision, and the wounds were exposed to natural infestations of C. hominivorax. Wound inspections were carried out on days 3, 6, and 13 post-treatments. Jugular blood samples were taken from experimental animals at 3- and 6-days post-treatment. In presence of C. hominivorax larvae, samples of both the larvae and wound secretions were collected in plastic vials on days 3 and 6 to measure concentrations of both drugs by high-performance liquid chromatography. On day 3 post-treatment, active myiasis was observed in 9 animals from the control group, 5 from the IVM 3.15 % group, and 6 from the DRM 1 % group. On day 6 post-treatment, 5 and 3 new myiasis were detected in the IVM 3.15 % and DRM 1 % group, respectively. No larvae were observed in the wounds on day 13 post-treatment. Interestingly, DRM concentrations tended to be higher in larvae compared to IVM on day 3 post-treatment (p = 0.051), and IVM plasma concentrations tended to be higher than those measured for DRM on day 6 (p = 0.087). There was a very strong correlation between DRM and IVM concentrations in plasma and wound secretions and larvae. The uptake of DRM from wound secretions to larvae was 83 % greater than that of IVM (p = 0.03). The results of this trial suggest the presence of C. hominivorax resistance to DRM and highlight concern about the lack of effectiveness of IVM 3.15 % in preventing C. hominivorax infestations. |
| format | Artículo |
| id | INTA21628 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA216282025-03-12T13:10:10Z Failure of doramectin and ivermectin in preventing Cochliomyia hominivorax myiasis in a subtropical region: A pharmacokinetic-pharmacodynamic study Muchiut, Sebastian Miró, Maria Victoria Anziani, Oscar Sergio Nava, Santiago Lifschitz, Adrian Luis Cochliomyia hominivorax Insects Ivermectin Cattle Pharmacokinetics Pharmacodynamics Insectos Ivermectina Ganado Bovino Farmacocinética Farmacodinámica Doramectina Doramectin The aim of this work is to present a case study where the failure of IVM 3.15 % and DRM 1 % to prevent natural infestations of C. hominivorax larvae in Argentina is investigated based on field efficacy tests and a pharmacokinetic and pharmacodynamic analysis. Thirty male crossbred Braford calves were randomly assigned to three experimental groups (n = 10), the IVM 3.15 % group (subcutaneously at 630 µg/kg), the DRM 1 % group (subcutaneously at 200 µg/kg) and the control group (saline solution subcutaneously). All treatments were performed at the time of castration surgery through a scrotal incision, and the wounds were exposed to natural infestations of C. hominivorax. Wound inspections were carried out on days 3, 6, and 13 post-treatments. Jugular blood samples were taken from experimental animals at 3- and 6-days post-treatment. In presence of C. hominivorax larvae, samples of both the larvae and wound secretions were collected in plastic vials on days 3 and 6 to measure concentrations of both drugs by high-performance liquid chromatography. On day 3 post-treatment, active myiasis was observed in 9 animals from the control group, 5 from the IVM 3.15 % group, and 6 from the DRM 1 % group. On day 6 post-treatment, 5 and 3 new myiasis were detected in the IVM 3.15 % and DRM 1 % group, respectively. No larvae were observed in the wounds on day 13 post-treatment. Interestingly, DRM concentrations tended to be higher in larvae compared to IVM on day 3 post-treatment (p = 0.051), and IVM plasma concentrations tended to be higher than those measured for DRM on day 6 (p = 0.087). There was a very strong correlation between DRM and IVM concentrations in plasma and wound secretions and larvae. The uptake of DRM from wound secretions to larvae was 83 % greater than that of IVM (p = 0.03). The results of this trial suggest the presence of C. hominivorax resistance to DRM and highlight concern about the lack of effectiveness of IVM 3.15 % in preventing C. hominivorax infestations. EEA Rafaela Fil: Muchiut, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Muchiut, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Miró, María Victoria. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Miró, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Miró, María Victoria. Provincia de Buenos Aires. Comisión de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Anziani, Oscar Sergio. Universidad Católica de Córdoba. Facultad Ciencias Agropecuarias. Laboratorio de Parasitología y Enfermedades Parasitarias; Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Lifschitz, Adrian. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina. Fil: Lifschitz, Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación Veterinaria de Tandil (CIVETAN); Argentina Fil: Lifschitz, Adrian. Provincia de Buenos Aires. Comisión de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina 2025-03-12T13:07:25Z 2025-03-12T13:07:25Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21628 https://www.sciencedirect.com/science/article/abs/pii/S0304401724002735 0304-4017 1873-2550 https://doi.org/10.1016/j.vetpar.2024.110384 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Parasitology 334 : 110384. (February 2025) |
| spellingShingle | Cochliomyia hominivorax Insects Ivermectin Cattle Pharmacokinetics Pharmacodynamics Insectos Ivermectina Ganado Bovino Farmacocinética Farmacodinámica Doramectina Doramectin Muchiut, Sebastian Miró, Maria Victoria Anziani, Oscar Sergio Nava, Santiago Lifschitz, Adrian Luis Failure of doramectin and ivermectin in preventing Cochliomyia hominivorax myiasis in a subtropical region: A pharmacokinetic-pharmacodynamic study |
| title | Failure of doramectin and ivermectin in preventing Cochliomyia hominivorax myiasis in a subtropical region: A pharmacokinetic-pharmacodynamic study |
| title_full | Failure of doramectin and ivermectin in preventing Cochliomyia hominivorax myiasis in a subtropical region: A pharmacokinetic-pharmacodynamic study |
| title_fullStr | Failure of doramectin and ivermectin in preventing Cochliomyia hominivorax myiasis in a subtropical region: A pharmacokinetic-pharmacodynamic study |
| title_full_unstemmed | Failure of doramectin and ivermectin in preventing Cochliomyia hominivorax myiasis in a subtropical region: A pharmacokinetic-pharmacodynamic study |
| title_short | Failure of doramectin and ivermectin in preventing Cochliomyia hominivorax myiasis in a subtropical region: A pharmacokinetic-pharmacodynamic study |
| title_sort | failure of doramectin and ivermectin in preventing cochliomyia hominivorax myiasis in a subtropical region a pharmacokinetic pharmacodynamic study |
| topic | Cochliomyia hominivorax Insects Ivermectin Cattle Pharmacokinetics Pharmacodynamics Insectos Ivermectina Ganado Bovino Farmacocinética Farmacodinámica Doramectina Doramectin |
| url | http://hdl.handle.net/20.500.12123/21628 https://www.sciencedirect.com/science/article/abs/pii/S0304401724002735 https://doi.org/10.1016/j.vetpar.2024.110384 |
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