Modeling tobacco mosaic virus proliferation in protoplasts
The tobacco mosaic virus (TMV) is one of the most studied viruses. It is frequently used as a model in the research of virus-host interactions. The interest in understanding the mechanism of its proliferation stems basically from the field of agriculture, due to the detrimental effect this virus has...
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| Format: | Artículo |
| Language: | Inglés |
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2018
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| Online Access: | http://hdl.handle.net/20.500.12123/2161 http://www.naun.org/main/NAUN/bio/2016/a462010-066.pdf |
| _version_ | 1855483005456351232 |
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| author | Merchuk, Jose C. Sánchez Mirón, Asterio Asurmendi, Sebastian Shacham, Mordechai |
| author_browse | Asurmendi, Sebastian Merchuk, Jose C. Shacham, Mordechai Sánchez Mirón, Asterio |
| author_facet | Merchuk, Jose C. Sánchez Mirón, Asterio Asurmendi, Sebastian Shacham, Mordechai |
| author_sort | Merchuk, Jose C. |
| collection | INTA Digital |
| description | The tobacco mosaic virus (TMV) is one of the most studied viruses. It is frequently used as a model in the research of virus-host interactions. The interest in understanding the mechanism of its proliferation stems basically from the field of agriculture, due to the detrimental effect this virus has on several crops. In addition to this direct application, virus-mediated protein expression systems, which are well established for the synthesis of foreign proteins in animal cell cultures, are now being applied to plants and plant cells by means of plant viral vectors. The use of transformed roots for the propagation of viral vectors has also been proposed. This work presents a mechanistic model describing the transient process of TMV multiplication in a protoplast (a wall-deprived cell). It aims to be a mathematical tool able to simulate the transient behavior of the main molecular pools taking part in the process, which will be useful for exploring, understanding and predicting the dynamics of a host-virus system. The variables considered are the pools of the main molecules taking part in the viral replication process. The basic balance equations for the cellular pools are presented and a satisfactory fit of the model to the experimental data is shown. The presented model is a necessary step toward the formulation of a basic mechanistic model for the systemic propagation of the virus in a plant tissue. It may be extended in many directions as to the optimization of a system for the production of a foreign protein, to the simulation of manipulation of the virus-cell interaction by external factors, to the mechanism of gene silencing or to the prediction of co-infection dynamics. |
| format | Artículo |
| id | INTA2161 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA21612018-04-04T17:27:29Z Modeling tobacco mosaic virus proliferation in protoplasts Merchuk, Jose C. Sánchez Mirón, Asterio Asurmendi, Sebastian Shacham, Mordechai Virus de las Plantas Nicotiana Tabacum Tabaco Modelos de Simulación Virosis Viroses Viruses Simulation Models Tobacco Plant Viruses Virus del Mosaico del Tabaco Virus Propagation The tobacco mosaic virus (TMV) is one of the most studied viruses. It is frequently used as a model in the research of virus-host interactions. The interest in understanding the mechanism of its proliferation stems basically from the field of agriculture, due to the detrimental effect this virus has on several crops. In addition to this direct application, virus-mediated protein expression systems, which are well established for the synthesis of foreign proteins in animal cell cultures, are now being applied to plants and plant cells by means of plant viral vectors. The use of transformed roots for the propagation of viral vectors has also been proposed. This work presents a mechanistic model describing the transient process of TMV multiplication in a protoplast (a wall-deprived cell). It aims to be a mathematical tool able to simulate the transient behavior of the main molecular pools taking part in the process, which will be useful for exploring, understanding and predicting the dynamics of a host-virus system. The variables considered are the pools of the main molecules taking part in the viral replication process. The basic balance equations for the cellular pools are presented and a satisfactory fit of the model to the experimental data is shown. The presented model is a necessary step toward the formulation of a basic mechanistic model for the systemic propagation of the virus in a plant tissue. It may be extended in many directions as to the optimization of a system for the production of a foreign protein, to the simulation of manipulation of the virus-cell interaction by external factors, to the mechanism of gene silencing or to the prediction of co-infection dynamics. Fil: Merchuk, Jose C. Ben-Gurion University of the Negev; Israel Fil: Sánchez-Mirón, Asterio. Universidad de Almería; España Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Shacham, Mordechai. Ben-Gurion University of the Negev; Israel 2018-04-04T13:07:50Z 2018-04-04T13:07:50Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/2161 http://www.naun.org/main/NAUN/bio/2016/a462010-066.pdf 1998-4510 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf International journal of biology and biomedical engineering 10 : 202-210. (2016) |
| spellingShingle | Virus de las Plantas Nicotiana Tabacum Tabaco Modelos de Simulación Virosis Viroses Viruses Simulation Models Tobacco Plant Viruses Virus del Mosaico del Tabaco Virus Propagation Merchuk, Jose C. Sánchez Mirón, Asterio Asurmendi, Sebastian Shacham, Mordechai Modeling tobacco mosaic virus proliferation in protoplasts |
| title | Modeling tobacco mosaic virus proliferation in protoplasts |
| title_full | Modeling tobacco mosaic virus proliferation in protoplasts |
| title_fullStr | Modeling tobacco mosaic virus proliferation in protoplasts |
| title_full_unstemmed | Modeling tobacco mosaic virus proliferation in protoplasts |
| title_short | Modeling tobacco mosaic virus proliferation in protoplasts |
| title_sort | modeling tobacco mosaic virus proliferation in protoplasts |
| topic | Virus de las Plantas Nicotiana Tabacum Tabaco Modelos de Simulación Virosis Viroses Viruses Simulation Models Tobacco Plant Viruses Virus del Mosaico del Tabaco Virus Propagation |
| url | http://hdl.handle.net/20.500.12123/2161 http://www.naun.org/main/NAUN/bio/2016/a462010-066.pdf |
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