Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization
Background and aims: Nitrogen (N) and phosphorus (P) fertilisers are widely used in agro-ecosystems but can endanger the diversity of beneficial soil-borne biota. This study aimed to determine the impact of long-term N and P fertilisation on arbuscular mycorrhizal fungi (AMF), a group of symbiotic s...
| Main Authors: | , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Springer Nature
2025
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/21601 https://link.springer.com/article/10.1007/s11104-024-07186-5 https://doi.org/10.1007/s11104-024-07186-5 |
| _version_ | 1855038427632762880 |
|---|---|
| author | Gaset, Braian Rubio, Gerardo Ferrari, Manuel Carlos Cavigliasso, Pablo Nishinakamasu, Veronica Puebla, Andrea Fabiana Faggioli, Valeria Soledad |
| author_browse | Cavigliasso, Pablo Faggioli, Valeria Soledad Ferrari, Manuel Carlos Gaset, Braian Nishinakamasu, Veronica Puebla, Andrea Fabiana Rubio, Gerardo |
| author_facet | Gaset, Braian Rubio, Gerardo Ferrari, Manuel Carlos Cavigliasso, Pablo Nishinakamasu, Veronica Puebla, Andrea Fabiana Faggioli, Valeria Soledad |
| author_sort | Gaset, Braian |
| collection | INTA Digital |
| description | Background and aims: Nitrogen (N) and phosphorus (P) fertilisers are widely used in agro-ecosystems but can endanger the diversity of beneficial soil-borne biota. This study aimed to determine the impact of long-term N and P fertilisation on arbuscular mycorrhizal fungi (AMF), a group of symbiotic soil fungi, by distinguishing between the effects of individual and combined nutrient applications.
Methods: We examined the impacts of long-term (i.e. 19 years) N and P fertilisation at two developmental crop stages: V6 (six fully expanded leaves) and R1 (initiation of flowering, after N addition). We measured mycorrhizal colonisation to test the plant-AMF relationship and used Illumina MiSeq sequencing of 18S rRNA gene from rhizospheric soil to evaluate AMF diversity.
Results: N and P fertilisation resulted primarily in additive effects rather than interactive effects. N fertilisation greatly increased alpha diversity (e.g. + 38% Chao2 at R1 sampling time) and changed AMF community composition (presence-absence data at R1). P fertilisation reduced mycorrhizal colonisation (~—8% at V6,—21% at R1), changed community composition (relative abundance data at V6 and R1) and negatively affected the abundance and richness of the predominant family Glomeraceae.
Conclusions: Long-term additions of N and P change AMF communities in distinct ways. While N mainly contributes to increases in alpha diversity, P influences the assembly of AMF by altering the dominance of major taxa within the community. Future studies are needed to disentangle the implications of these changes for crop yields and nutrient use efficiency to ensure the sustainability of agro-ecosystems. |
| format | info:ar-repo/semantics/artículo |
| id | INTA21601 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INTA216012025-03-10T10:43:15Z Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization Gaset, Braian Rubio, Gerardo Ferrari, Manuel Carlos Cavigliasso, Pablo Nishinakamasu, Veronica Puebla, Andrea Fabiana Faggioli, Valeria Soledad Maíz Nitrógeno Fósforo Agroecosistemas Sostenibilidad Argentina Maize Nitrogen Phosphorus Agroecosystems Sustainability Arbuscular Mycorrhiza Micorrizas Arbusculares Fertilización No-tillage Background and aims: Nitrogen (N) and phosphorus (P) fertilisers are widely used in agro-ecosystems but can endanger the diversity of beneficial soil-borne biota. This study aimed to determine the impact of long-term N and P fertilisation on arbuscular mycorrhizal fungi (AMF), a group of symbiotic soil fungi, by distinguishing between the effects of individual and combined nutrient applications. Methods: We examined the impacts of long-term (i.e. 19 years) N and P fertilisation at two developmental crop stages: V6 (six fully expanded leaves) and R1 (initiation of flowering, after N addition). We measured mycorrhizal colonisation to test the plant-AMF relationship and used Illumina MiSeq sequencing of 18S rRNA gene from rhizospheric soil to evaluate AMF diversity. Results: N and P fertilisation resulted primarily in additive effects rather than interactive effects. N fertilisation greatly increased alpha diversity (e.g. + 38% Chao2 at R1 sampling time) and changed AMF community composition (presence-absence data at R1). P fertilisation reduced mycorrhizal colonisation (~—8% at V6,—21% at R1), changed community composition (relative abundance data at V6 and R1) and negatively affected the abundance and richness of the predominant family Glomeraceae. Conclusions: Long-term additions of N and P change AMF communities in distinct ways. While N mainly contributes to increases in alpha diversity, P influences the assembly of AMF by altering the dominance of major taxa within the community. Future studies are needed to disentangle the implications of these changes for crop yields and nutrient use efficiency to ensure the sustainability of agro-ecosystems. EEA Pergamino Fil: Gaset, Braian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Manejo de cultivos; Argentina Fil: Rubio, Gerardo. Universidad de Buenos Aires. Facultad de Agronomía. Fertilidad de Suelos y Fertilizantes. Instituto de Investigaciones en Biociencias Agropecuarias y Ambientales; Argentina Fil: Ferrari, Manuel Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Producción Vegetal, Mejoramiento y Gestión Ambiental; Argentina Fil: Cavigliasso, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Nishinakamasu, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Nishinakamasu, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina Fil: Nishinakamasu, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nishinakamasu, Verónica. Unidad de Genómica y Bioinformática; Argentina Fil: Puebla, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Puebla, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina Fil: Puebla, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Puebla, Andrea. Unidad de Genómica y Bioinformática; Argentina Fil: Faggioli, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina 2025-03-10T10:27:14Z 2025-03-10T10:27:14Z 2025-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21601 https://link.springer.com/article/10.1007/s11104-024-07186-5 0032-079X 1573-5036 (online) https://doi.org/10.1007/s11104-024-07186-5 eng info:eu-repograntAgreement/INTA/2023-PD-L03-I093, Fertilidad química, física y biológica del suelo: estrategias para mitigar y restaurar procesos de degradación info:eu-repograntAgreement/INTA/2019-PD-E2-I037-002, Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso suelo info:eu-repograntAgreement/INTA/2019-PE-E1-I011-001, Intensificacion Sustentable de la Agricultura en la Region Pampeana info:eu-repograntAgreement/INTA/2019-RIST-E1-I503-001, Red de ensayos de larga duración info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Nature Plant and Soil 506 (1-2) : 1-18. (January 2025) |
| spellingShingle | Maíz Nitrógeno Fósforo Agroecosistemas Sostenibilidad Argentina Maize Nitrogen Phosphorus Agroecosystems Sustainability Arbuscular Mycorrhiza Micorrizas Arbusculares Fertilización No-tillage Gaset, Braian Rubio, Gerardo Ferrari, Manuel Carlos Cavigliasso, Pablo Nishinakamasu, Veronica Puebla, Andrea Fabiana Faggioli, Valeria Soledad Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization |
| title | Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization |
| title_full | Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization |
| title_fullStr | Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization |
| title_full_unstemmed | Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization |
| title_short | Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization |
| title_sort | nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization |
| topic | Maíz Nitrógeno Fósforo Agroecosistemas Sostenibilidad Argentina Maize Nitrogen Phosphorus Agroecosystems Sustainability Arbuscular Mycorrhiza Micorrizas Arbusculares Fertilización No-tillage |
| url | http://hdl.handle.net/20.500.12123/21601 https://link.springer.com/article/10.1007/s11104-024-07186-5 https://doi.org/10.1007/s11104-024-07186-5 |
| work_keys_str_mv | AT gasetbraian nitrogenenhancesdiversityofarbuscularmycorrhizalfungiwhilephosphorusdrivescommunitycompositioninmaizerhizosphereafter19yearsoffertilization AT rubiogerardo nitrogenenhancesdiversityofarbuscularmycorrhizalfungiwhilephosphorusdrivescommunitycompositioninmaizerhizosphereafter19yearsoffertilization AT ferrarimanuelcarlos nitrogenenhancesdiversityofarbuscularmycorrhizalfungiwhilephosphorusdrivescommunitycompositioninmaizerhizosphereafter19yearsoffertilization AT cavigliassopablo nitrogenenhancesdiversityofarbuscularmycorrhizalfungiwhilephosphorusdrivescommunitycompositioninmaizerhizosphereafter19yearsoffertilization AT nishinakamasuveronica nitrogenenhancesdiversityofarbuscularmycorrhizalfungiwhilephosphorusdrivescommunitycompositioninmaizerhizosphereafter19yearsoffertilization AT pueblaandreafabiana nitrogenenhancesdiversityofarbuscularmycorrhizalfungiwhilephosphorusdrivescommunitycompositioninmaizerhizosphereafter19yearsoffertilization AT faggiolivaleriasoledad nitrogenenhancesdiversityofarbuscularmycorrhizalfungiwhilephosphorusdrivescommunitycompositioninmaizerhizosphereafter19yearsoffertilization |