Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat
Several phytopathogenic bacteria have been reported worldwide affecting wheat (Triticum aestivum L.), causing significant yield losses. Over the past 5–6 years, symptoms resembling bacterial mosaic, characterised by small yellow lesions with indefinite margins scattered across the leaf, have been ob...
| Main Authors: | , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Wiley
2025
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| Online Access: | http://hdl.handle.net/20.500.12123/21578 https://onlinelibrary.wiley.com/doi/10.1111/jph.70049 https://doi.org/10.1111/jph.70049 |
| _version_ | 1855486778888159232 |
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| author | Fernandez, Franco Daniel Martino, Julia Andrea Pozzi, Elizabeth Alicia Alberione, Enrique Javier Tolocka, Patricia Marquez, Nathalie Rodriguez, Ana Valeria Conci, Luis Rogelio Alemandri, Vanina Maria |
| author_browse | Alberione, Enrique Javier Alemandri, Vanina Maria Conci, Luis Rogelio Fernandez, Franco Daniel Marquez, Nathalie Martino, Julia Andrea Pozzi, Elizabeth Alicia Rodriguez, Ana Valeria Tolocka, Patricia |
| author_facet | Fernandez, Franco Daniel Martino, Julia Andrea Pozzi, Elizabeth Alicia Alberione, Enrique Javier Tolocka, Patricia Marquez, Nathalie Rodriguez, Ana Valeria Conci, Luis Rogelio Alemandri, Vanina Maria |
| author_sort | Fernandez, Franco Daniel |
| collection | INTA Digital |
| description | Several phytopathogenic bacteria have been reported worldwide affecting wheat (Triticum aestivum L.), causing significant yield losses. Over the past 5–6 years, symptoms resembling bacterial mosaic, characterised by small yellow lesions with indefinite margins scattered across the leaf, have been observed in wheat plants in one of the main producing areas in Argentina, with an incidence of 10% to 20%. This emerging disease has raised concern among producers and technicians. In this study, a bacterium was isolated from symptomatic seeds, and biochemical, molecular, and genomic techniques were used to identify the causal agent. Pathogenicity tests were conducted on wheat and soybean plants through leaf infiltration, demonstrating that the strain caused disease in wheat but not in soybean. Using a combination of MALDI-TOF MS, the API 20 NE test, 16S rRNA gene, and whole-genome sequencing confirmed the pathogen as Curtobacterium flaccumfaciens. Phylogenomic and genome-to-genome comparisons showed the strain sequenced grouped within the type strain sequence of C. poinsettiae CFB 2403. To our knowledge, this is the first report of Curtobacterium flaccumfaciens as a pathogen on wheat in Argentina. This research contributes significantly to understanding this phytosanitary problem with detailed information on the pathogen, using advanced technology and new reclassifications of pathogenic bacteria, which will allow the development and implementation of effective management strategies in the future. |
| format | Artículo |
| id | INTA21578 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA215782025-03-07T10:11:17Z Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat Fernandez, Franco Daniel Martino, Julia Andrea Pozzi, Elizabeth Alicia Alberione, Enrique Javier Tolocka, Patricia Marquez, Nathalie Rodriguez, Ana Valeria Conci, Luis Rogelio Alemandri, Vanina Maria Cereals Genomes Seeds Wheat Pathogenic Bacteria Cereal Genoma Semilla Trigo Bacteria Patógena Bacterial Mosaic Curtobacterium flaccumfaciens Several phytopathogenic bacteria have been reported worldwide affecting wheat (Triticum aestivum L.), causing significant yield losses. Over the past 5–6 years, symptoms resembling bacterial mosaic, characterised by small yellow lesions with indefinite margins scattered across the leaf, have been observed in wheat plants in one of the main producing areas in Argentina, with an incidence of 10% to 20%. This emerging disease has raised concern among producers and technicians. In this study, a bacterium was isolated from symptomatic seeds, and biochemical, molecular, and genomic techniques were used to identify the causal agent. Pathogenicity tests were conducted on wheat and soybean plants through leaf infiltration, demonstrating that the strain caused disease in wheat but not in soybean. Using a combination of MALDI-TOF MS, the API 20 NE test, 16S rRNA gene, and whole-genome sequencing confirmed the pathogen as Curtobacterium flaccumfaciens. Phylogenomic and genome-to-genome comparisons showed the strain sequenced grouped within the type strain sequence of C. poinsettiae CFB 2403. To our knowledge, this is the first report of Curtobacterium flaccumfaciens as a pathogen on wheat in Argentina. This research contributes significantly to understanding this phytosanitary problem with detailed information on the pathogen, using advanced technology and new reclassifications of pathogenic bacteria, which will allow the development and implementation of effective management strategies in the future. Instituto de Patología Vegetal Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Martino, Julia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Martino, Julia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Pozzi, Elizabeth Alicia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina. Fil: Alberione, Enrique Javier. Instituto Nacional de Tecnología Agropecuaria (INTA).Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Tolocka, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Tolocka, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Marquez, Nathalie. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Rodriguez, Ana Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Conci, Luis Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Alemandri, Vanina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Alemandri, Vanina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina 2025-03-07T09:58:08Z 2025-03-07T09:58:08Z 2025-02-20 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21578 https://onlinelibrary.wiley.com/doi/10.1111/jph.70049 0931-1785 1439-0434 (online) https://doi.org/10.1111/jph.70049 eng info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo info:eu-repograntAgreement/INTA/2023-PD-L01-I083, Determinación de los mecanismos moleculares que rigen las interacciones entre plantas y patógenos y desarrollo de herramientas para contribuir al control de enfermedades info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Journal of Phytopathology 173 (2): e70049. (First published: 02 March 2025 ) |
| spellingShingle | Cereals Genomes Seeds Wheat Pathogenic Bacteria Cereal Genoma Semilla Trigo Bacteria Patógena Bacterial Mosaic Curtobacterium flaccumfaciens Fernandez, Franco Daniel Martino, Julia Andrea Pozzi, Elizabeth Alicia Alberione, Enrique Javier Tolocka, Patricia Marquez, Nathalie Rodriguez, Ana Valeria Conci, Luis Rogelio Alemandri, Vanina Maria Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat |
| title | Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat |
| title_full | Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat |
| title_fullStr | Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat |
| title_full_unstemmed | Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat |
| title_short | Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat |
| title_sort | biological and genomic characterisation of curtobacterium flaccumfaciens infecting wheat |
| topic | Cereals Genomes Seeds Wheat Pathogenic Bacteria Cereal Genoma Semilla Trigo Bacteria Patógena Bacterial Mosaic Curtobacterium flaccumfaciens |
| url | http://hdl.handle.net/20.500.12123/21578 https://onlinelibrary.wiley.com/doi/10.1111/jph.70049 https://doi.org/10.1111/jph.70049 |
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