Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential
Anastrepha fraterculus is a significant fruit fly pest in Argentina and other South American countries. Previous studies showed the key role of gut bacteria in the protection and nutrient assimilation of fruit flies, particularly the importance of the biological fixation of nitrogen (diazotrophy). T...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2025
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/21545 https://onlinelibrary.wiley.com/doi/10.1111/1744-7917.70004 https://doi.org/10.1111/1744-7917.70004 |
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| author | Salgueiro, Julieta Nussenbaum, Ana Laura Marchesini, María Inés Garbalena, Micaela Soledad Brambilla, Silvina Maricel Belliard, Silvina Ahnahi Cuadros, Fabián Núñez, Mauricio Yáñez, Carolina Juárez, María Laura Vera, María Teresa Lanzavecchia, Silvia Beatriz Tsiamis, George Segura, Diego Fernando |
| author_browse | Belliard, Silvina Ahnahi Brambilla, Silvina Maricel Cuadros, Fabián Garbalena, Micaela Soledad Juárez, María Laura Lanzavecchia, Silvia Beatriz Marchesini, María Inés Nussenbaum, Ana Laura Núñez, Mauricio Salgueiro, Julieta Segura, Diego Fernando Tsiamis, George Vera, María Teresa Yáñez, Carolina |
| author_facet | Salgueiro, Julieta Nussenbaum, Ana Laura Marchesini, María Inés Garbalena, Micaela Soledad Brambilla, Silvina Maricel Belliard, Silvina Ahnahi Cuadros, Fabián Núñez, Mauricio Yáñez, Carolina Juárez, María Laura Vera, María Teresa Lanzavecchia, Silvia Beatriz Tsiamis, George Segura, Diego Fernando |
| author_sort | Salgueiro, Julieta |
| collection | INTA Digital |
| description | Anastrepha fraterculus is a significant fruit fly pest in Argentina and other South American countries. Previous studies showed the key role of gut bacteria in the protection and nutrient assimilation of fruit flies, particularly the importance of the biological fixation of nitrogen (diazotrophy). The presence of diazotrophic bacteria in A. fraterculus sp. 1 has been demonstrated through molecular, culture-independent methods. This study is aimed to characterize the composition and diversity of culturable gut bacteria of A. fraterculus sp. 1 males from different origins, and explore their metabolic roles, focusing on diazotrophic bacteria. Three male groups were studied: wild-caught (WW), lab-reared from wild larvae (WL), and lab-colony raised (LL). Gut bacteria were collected and characterized via 16S rRNA gene sequencing, with potential diazotrophs screened using selective media (SIL and NFb). Phylogenetic analysis of 16S rRNA gene mapped potential diazotrophs across the bacterial collection, while biochemical profiling and ARDRA (Amplified rDNA Restriction Analysis) were used to quickly differentiate diazotrophic bacteria. PCR testing for the nifH gene, associated with nitrogen fixation, was also performed. Bacterial diversity was highest in WW, followed by WL, and lowest in LL. In LL and WL, Enterobacter was the most frequent genus, while Klebsiella dominated in WW. Among the 20 SIL+ isolates identified, 10 came from WW, 9 from WL, and 1 from LL. One of these isolates (Enterobacter sp.) was tested as a supplement to the adult diet, without showing a beneficial effect on males pheromone calling behavior. Three isolates were also NFb+; two had the nifH gene. ARDRA was effective for rapid diazotroph discrimination. These findings highlight the potential of gut symbiotic bacteria in eco-friendly pest management strategies like the sterile insect technique (SIT). By using diazotrophic bacteria, protein requirements in artificial diets could be reduced, cutting costs and improving the affordability of SIT programs. |
| format | Artículo |
| id | INTA21545 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA215452025-03-05T14:53:28Z Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential Salgueiro, Julieta Nussenbaum, Ana Laura Marchesini, María Inés Garbalena, Micaela Soledad Brambilla, Silvina Maricel Belliard, Silvina Ahnahi Cuadros, Fabián Núñez, Mauricio Yáñez, Carolina Juárez, María Laura Vera, María Teresa Lanzavecchia, Silvia Beatriz Tsiamis, George Segura, Diego Fernando Anastrepha fraterculus Nitrogen Fixation Symbionts Nitrogen-fixing Bacteria Tephritidae Sterile Insect Release Bacteria Fijación del Nitrógeno Simbiontico Bacteria Fijadora del Nitrógeno Liberación de Insectos Estériles Anastrepha fraterculus is a significant fruit fly pest in Argentina and other South American countries. Previous studies showed the key role of gut bacteria in the protection and nutrient assimilation of fruit flies, particularly the importance of the biological fixation of nitrogen (diazotrophy). The presence of diazotrophic bacteria in A. fraterculus sp. 1 has been demonstrated through molecular, culture-independent methods. This study is aimed to characterize the composition and diversity of culturable gut bacteria of A. fraterculus sp. 1 males from different origins, and explore their metabolic roles, focusing on diazotrophic bacteria. Three male groups were studied: wild-caught (WW), lab-reared from wild larvae (WL), and lab-colony raised (LL). Gut bacteria were collected and characterized via 16S rRNA gene sequencing, with potential diazotrophs screened using selective media (SIL and NFb). Phylogenetic analysis of 16S rRNA gene mapped potential diazotrophs across the bacterial collection, while biochemical profiling and ARDRA (Amplified rDNA Restriction Analysis) were used to quickly differentiate diazotrophic bacteria. PCR testing for the nifH gene, associated with nitrogen fixation, was also performed. Bacterial diversity was highest in WW, followed by WL, and lowest in LL. In LL and WL, Enterobacter was the most frequent genus, while Klebsiella dominated in WW. Among the 20 SIL+ isolates identified, 10 came from WW, 9 from WL, and 1 from LL. One of these isolates (Enterobacter sp.) was tested as a supplement to the adult diet, without showing a beneficial effect on males pheromone calling behavior. Three isolates were also NFb+; two had the nifH gene. ARDRA was effective for rapid diazotroph discrimination. These findings highlight the potential of gut symbiotic bacteria in eco-friendly pest management strategies like the sterile insect technique (SIT). By using diazotrophic bacteria, protein requirements in artificial diets could be reduced, cutting costs and improving the affordability of SIT programs. Instituto de Genética Fil: Salgueiro, Julieta. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS); Argentina Fil: Salgueiro, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS); Argentina Fil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Nussenbaum, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nussenbaum, Ana Laura. Universidad del Salvador. Facultad de Ciencias Agronómicas y Veterinarias; Argentina Fil: Marchesini, María Inés. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Marchesini, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garbalena, Micaela Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina Fil: Garbalena, Micaela Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Garbalena, Micaela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Brambilla, Silvina Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Belliard, Silvina Ahnahi. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental (DBBE); Argentina Fil: Cuadros, Fabián. Pontificia Universidad Católica de Valparaíso. Facultad de Ciencias. Instituto de Biología. Laboratorio de Microbiología; Chile Fil: Núñez, Mauricio. Pontificia Universidad Católica de Valparaíso. Facultad de Ciencias. Instituto de Biología. Laboratorio de Microbiología; Chile Fil: Yáñez, Carolina. Pontificia Universidad Católica de Valparaíso. Facultad de Ciencias. Instituto de Biología. Laboratorio de Microbiología; Chile Fil: Juárez, María Laura. Fundación Miguel Lillo. Unidad Ejecutora Lillo; Argentina Fil: Juárez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vera, María Teresa. Joint FAO/IAEA Center of Nuclear Techniques in Food and Agriculture. Insect Pest Control Laboratory; Austria Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tsiamis, George. University of Patras. Department of Environmental Engineering; Grecia Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Segura, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Segura, Diego Fernando. Universidad del Salvador. Facultad de Ciencias Agronómicas y Veterinarias; Argentina 2025-03-05T14:43:30Z 2025-03-05T14:43:30Z 2025-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21545 https://onlinelibrary.wiley.com/doi/10.1111/1744-7917.70004 1744-7917 https://doi.org/10.1111/1744-7917.70004 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Insect Science : 1-20 (First published: 24 February 2025) |
| spellingShingle | Anastrepha fraterculus Nitrogen Fixation Symbionts Nitrogen-fixing Bacteria Tephritidae Sterile Insect Release Bacteria Fijación del Nitrógeno Simbiontico Bacteria Fijadora del Nitrógeno Liberación de Insectos Estériles Salgueiro, Julieta Nussenbaum, Ana Laura Marchesini, María Inés Garbalena, Micaela Soledad Brambilla, Silvina Maricel Belliard, Silvina Ahnahi Cuadros, Fabián Núñez, Mauricio Yáñez, Carolina Juárez, María Laura Vera, María Teresa Lanzavecchia, Silvia Beatriz Tsiamis, George Segura, Diego Fernando Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential |
| title | Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential |
| title_full | Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential |
| title_fullStr | Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential |
| title_full_unstemmed | Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential |
| title_short | Culturable bacteria associated with Anastrepha fraterculus sp. 1 : in search of nitrogen-fixing symbionts with biotechnological potential |
| title_sort | culturable bacteria associated with anastrepha fraterculus sp 1 in search of nitrogen fixing symbionts with biotechnological potential |
| topic | Anastrepha fraterculus Nitrogen Fixation Symbionts Nitrogen-fixing Bacteria Tephritidae Sterile Insect Release Bacteria Fijación del Nitrógeno Simbiontico Bacteria Fijadora del Nitrógeno Liberación de Insectos Estériles |
| url | http://hdl.handle.net/20.500.12123/21545 https://onlinelibrary.wiley.com/doi/10.1111/1744-7917.70004 https://doi.org/10.1111/1744-7917.70004 |
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